Organic Molecules Flashcards
called molecules of life
organic compounds
tests can be used to detect the presence of
lipids
proteins
carbohydrates
test used to detect the presence of reducing sugars
Benedict’s Test
materials needed for Benedict’s Test
Benedict solution
beaker with water
water bath
how to do Benedict’s Test
mix the benedict’s solution with the solution to be tested and place under water bath
results of Benedict’s test when there is no reducing sugar
color blue
results of Benedict’s test when there is high traces of reducing sugar
color brick red
range of colors from no reducing sugar to high
blue - green - yellow - orange - red
used to test the starch in the solution
Iodine test
materials needed for Iodine test
iodine solution
how to do the iodine test
add a few drops of iodine into the solution
result of iodine when there is no starch
brown/yellow
result of iodine when there is starch
blue/purple
used to test the presence of proteins
Biuret’s Test
materials needed for Biuret’s test
1mL of the solution
4mL of 10% NaOH (Sodium hydroxide)
few drops of 1% CuSO (Copper sulfate)
how to do Biuret’s Test
place 4mL of 10% Sodium Hydroxide with 1mL of solution to be tested, add a few drops of 1% Copper sulfate
negative Biuret’s test is color
blue
positive biuret’s test is colored
deep purple
used to test the presence of lipid
lipid test
material needed for lipid test
filter paper
how to do lipid test
place a drop in the filter paper, air dry
result when lipid is present
translucent spot in the filter paper
result when lipid is negative
no spot in the filter paper
potato when tested for organic compound
Benedict (negative)
Iodine (Positive)
Biuret (negative)
Spot (negative)
bread when tested for organic compound
Benedict (positive)
Iodine (positive)
Biuret (positive)
Lipid (negative)
Pineapple when tested for organic compound
Benedict (positive)
Iodine (negative)
Biuret (negative)
Lipid (negative)
Butter when tested for organic compounds
Benedict (negative)
Iodine (negative)
Biuret (positive)
Lipid (positive)
Egg when tested for organic compounds
Benedict (negative)
Iodine (negative)
Biuret (positive)
Lipid (positive)
most abundant and diverse class of organic compounds occurring in nature
carbohydates
carbohydrates are defined as
polyhdroxy aldehydes or ketones
four chemical groups of carbohydrates
monosaccharides, disaccharides, oligosaccharides, polysaccharides
difference between ketone and aldehyde
oxygen is located on internal carbon rather than terminal one
non reducing sugars do not have
OH group
test for specific carbohydrates
Molisch’s Test