organ bath experimentation

Question 1

What is a organ bath experiment?

Answer 1

An experiment that provides a biological model system that exist without the systemic influences of the intact animal

Question 2

What does organ bath preps allow?

Answer 2

Enables to define the efficacy and potency of a drug at a defined target the selectivity of molecule

Question 3

Give example if two commonly used tissues/organs used?

Answer 3

Ileum, arterial rings or steps ect.

Question 4

What are the recorded responses or biological activity?

Answer 4

Usually contraction/relaxation of muscle in the tissue: Force (isometric), Displacement (isotonic)

Question 5

What is the advantages of this method?

Answer 5

Ease in preparation
Minimal equipment requirements and facts that reproducible concentration-effect curves can be obtained using either contractile or relaxant agents

Question 6

Why do we need reproducible response?

Answer 6

Numbers
Biological variation
Experimental error-equipment and human

Question 7

What must not happen when recycling whole blood from donors using peristaltic pump through a membrane oxygenator for exp.?

Answer 7

Must be no denaturing blood proteins or causing erythrocytes damage

Question 8

What are limitations of whole blood use?

Answer 8

May contain biologically active materials, such as hormone and various cell types that may affect the tissue prep

Question 9

What are artificial plasma-like buffer solution?

Answer 9

Water based must contain no contaminants. Some recipes for buffer solutions still ads 0.1-5 EDTA for creation of heavy metals that may be added form salts or leached form metal tubing or other sources.

Question 10

List some buffer solutions?

Answer 10

Ringers
Tyrodes
Krebs-Hanseleit

Question 11

Why is 5% CO2/95% O2 used in bicarbonate buffers such as tyrodes and Krebs?

Answer 11

They are effective in equilibrium with relatively high levels of carbon dioxide

Question 12

Why is 95-100% O2 used instead of atmospheric levels of 20%?

Answer 12

To increase oxygen content of the solution to compensate for the lack of haemoglobin or other oxygen carriers. Phosphates are added to most.

Question 13

Why are carbonates, bicarbonates and phosphates added?

Answer 13

Besides adjusting the pH and increasing buffering capacity, they aid in maintaining normal an ion homeostasis