opportunistic mycoses Flashcards
midterms
refers to the free-living microorganisms in the environment that are not typically of concern in human disease
saprobe / saprophyte
opportunistic mycsoses are seen in ____ commonly encountered as ___
opportunistic mycsoses are seen in immunocompromised patients commonly encountered as saprobes
most commonly encountered fungi in the lab, common in peanuts
aspergillus
second most isolated fungus after candida spp.
aspergillus
aspergillus is transmitted by?
inhalation of fungal conidia
septate hyphae branching of aspergillus
45 degrees
aspergillus
Infection is initiated following inhalation of ___. In the lung air spaces, conidia germinate and invade the __.
Infection is initiated following inhalation of fungal conidia. In the lung air spaces, conidia germinate and invade the tissue.
clinical manifestations of aspergillus
- pulmonary or sinus fungus balls
- allergic bronchopulmonary aspergillosis
- external otomycosis
- mycotic keratitis
- onychomycosis
- sinuisitis
- endocarditis
clinical manifestation of aspergillus in the nail and surrounding tissues
onychomycosis
clinical manifestation of aspergillus in the auditory canal
external otomycosis
In terms of structure, aspergillus has a __ and __.
septated hyphae and conidiophore
this can be seen in at the end of the conidiophore of aspergillus
vesicle
a supporting structure that attached to the vesicles of aspergillus where the phialides attached
mutulae
produced from the phialides of aspergillus
conidia
has an an erect conidiophore arising from a foot cell within the vegetative hyphae.
aspergillus
lab diagnosis of aspergillus
- antigen-protein based assays
- nucleic acid amplification assay
for monitoring patients who are at high risk of developing invasive fungal infections
antigen-protein based assays
downside of antigen-protein based assays
not true to all spp. of aspergillus because it can have cross reactions
downside of nucleic acid amplification assay
not readily available for fungi in general
rapid grower, has a blue-green powdery appearance, and thermotolerant at 45C
Aspergillus fumigatus
A. fumigatus grows in ___ days
2-6 days
microscopic apperance:
- septate hyphae
- foot cell at base L or T shaped
A. fumigatus
colony of A. flavus
yellow-green colony
A. flavus grows in ____ days
1-5 days
microscopic appearance:
- phialides are uniseriate and biseriate
- Phialides give rise to short chains of yellow orange elliptical or spherical conidia
A. flavus
phialides that produced directly from the vesicle
uniseriate
phialides that produced from a primary row of cells called metulae
biseriate
structure: septate hyphae, long conidiophores, large metulae, small phialides (biseriate)
identify!
A. niger