(OLD) (Dr. Choy) (Unit A) Topic 4: Protein Function (Unfinished)

Question 1

What functions can proteins have?

Answer 1

• Enzymes
• Structural
• Transport
• Motor
• Storage
• Signal
• Receptor
• Gene regulator

Question 2

What forms of post-translation modifications (PMTs) are there?

Answer 2

1. Phosphorylation, Ubiquitination, Acetylation, Sumoylation
2. Glycosylation
3. Lipoproteins
4. Hemoproteins

Question 3

Where does phosphorylation occur?

Answer 3

On hydroxide groups of serine, tyrosine, theonine etc.

Question 4

Describe:

Protein-Ligand Binding

Answer 4

Proteins contain sites which ligand specifically bind and form complex with the protein

Occurs
* Multiple weak forces
* Few strong forces

Question 5

What is Kd?

Answer 5

Dissociation constant
* A measure of strength or affinity of interaction between protein and ligand
* Kd is big = Interaction is weak
* Kd is small = Interaction is strong