OECD 207 Flashcards
What is the prerequisite data needed for OECD/OCDE 207?
- Water solubility
- Vapour pressure
- Structural formula
- Purity and stability
- n-Octanol/water partition coefficient
- Biodegradability data
These data points are essential for assessing the toxicity of substances to earthworms.
What types of substances can the OECD 207 test be used for?
Water-soluble and insoluble substances
This applicability allows for a wider range of chemical testing.
What does LC50 stand for in the context of earthworm toxicity tests?
Median lethal concentration (kills 50% of worms)
LC50 is a key measure used in toxicity testing.
What is the reference substance suggested for the OECD 207 test?
Chloracetamide
Reference substances are used to compare results across tests.
What are the required materials for the Artificial Soil Test in OECD 207?
- Filter paper
- Artificial soil (10% peat, 20% kaolin clay, 70% sand)
- Moisture content ~35%
- pH adjusted to 6.0 ± 0.5
- Test containers with lids
These materials simulate a natural environment for the worms.
What is the preferred strain of earthworm for testing in OECD 207?
E. foetida foetida (striped)
This strain is specifically chosen for its characteristics suitable for testing.
What is the exposure duration for the Filter Paper Test in OECD 207?
48 hours (optional check at 72 hours)
This duration helps assess the immediate effects of toxic substances.
What is the temperature condition required during the tests in OECD 207?
20 ± 2°C
Temperature control is critical for maintaining consistent testing conditions.
What type of analysis is used to calculate LC50 values in OECD 207?
Probit analysis
This statistical method transforms data for clearer interpretation of toxicity.
What is the best breeding medium for earthworms mentioned in OECD 207?
50:50 mix of horse/cattle manure and peat
The right breeding medium is crucial for the health and reproduction of earthworms.
True or False: Earthworms can be kept in dry conditions during toxicity testing.
False
Earthworms require a moist environment for biological functions.
Fill in the blank: The test species used in OECD 207 is _______.
Eisenia foetida
This species is commonly used in ecotoxicological studies.
What is the optimal pH for breeding earthworms according to OECD 207?
~7.0
Maintaining optimal pH is essential for successful breeding.
What is the typical weight range for adult earthworms used in OECD 207 tests?
300–600 mg
This weight range ensures that the worms are mature enough for testing.
What is an example of a geometric series of doses for toxicity testing?
0.1, 1, 10, 100
A geometric series is important for assessing dose-response relationships.
What statistical tool is used to determine LC50 concentrations?
Probit analysis
Probit analysis is crucial for evaluating the toxicity of substances.
What type of acute toxicity test is generally recommended first by OECD if low toxicity is expected?
Limit test
This approach is often used to confirm low toxicity before conducting more extensive testing.
