Observing Mitosis Flashcards
What is the equation for Total Magnification?
Total Magnification= Eyepiece lens magnification + Objective lens magnification
What are the first 3 steps of using a light microscope?
1st: Take your slide with your sample and place it on the stage.
2nd: Set the microscope to the lowest magnification objective lens.
3rd: Turn the coarse focusing knob until the cells come into focus
What are the last 3 steps of using a light microscope?
4th: Turn the fine focusing knob to clearly focus the cells
5th: Increase the magnification by turning the objective lens
6th: Refocus the cells using the fine focusing knob
What tissues to use to observe mitosis?
Meristem
Where are meristem found?
Roots and shoot tips
Why is Toluidine blue stain used?
It binds to the chromatin DNA which stains it deep blue and makes the chromosomes visible
Why are the root tips suspended in water?
The water can help to elongate the cells as it is absorbed by osmosis causing the cells to swell. This can separate the chromosomes and make them easier to see.
Why is acid used?
Breaks down the cells walls and make them more permeable to allow the stain to penetrate the cell and reach the chromosomes.
Why is the coverslip pressed gently on the slide?
To produce a layer that is 1 cell thick so light can pass through
Why should press down firmly instead of sliding the coverslip?
Prevents 1 layer of cells from rolling on top of another. Prevents chromosomes from being damaged
