Nutrition and Metabolism Flashcards

1
Q

What is a nutrient?

A

Nutrient: a substance in food that promotes normal growth, maintenance, and repair of cells

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2
Q

Some nutrients include…

A

Nutrients include carbohydrates, lipids, proteins, vitamins, minerals (and water)

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3
Q

What is an essential nutrient?

A

A nutrient that cannot be synthesized by the body, and thus must be obtained from a dietary source

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4
Q

What is a calorie?

A

The calorie (cal) is the amount of energy needed to raise the temperature of one gram of water by one degree Celsius

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5
Q

What is a food calorie?

A

The food calorie (Cal) is defined in terms of the kilogram rather than the gram. It is equal to 1000 calories (kcal)

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6
Q

Why are carbohydrates important?

A

a. Glucose is the fuel used by cells to make ATP
i. Neurons and RBCs rely almost entirely upon glucose
ii. Excess glucose is converted and stored as glycogen or fat

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7
Q

What are sugars?

A

a. Sugars (simple carbohydrates – usually mono- or disacharides)
b. Sugar, jams, fruit drinks, honey, etc

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8
Q

What is starch?

A

a. Starch (complex carbohydrates)

b. Green vegetables, grains, starchy vegetables, beans, lentils

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9
Q

What are two types of fibers?

A

a. Insoluble fiber: cellulose in vegetables; provides roughage
b. Soluble fiber: pectin in apples and citrus fruits; reduces blood cholesterol levels

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10
Q

What are lipids? Name and describe three types.

A

a. Triglycerides and fatty acids
b. Saturated fatty acids found in meat, dairy foods, and tropical oils (coconut, palm kernel or palm oils)
c. Unsaturated fats found in seeds, nuts, olive oil, and most vegetable oils (corn, peanut, soybean or sunflower oils)
d. Essential fatty acids
i. Linoleic and linolenic acid, found in most vegetable oils
ii. Must be ingested in diet

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11
Q

What is the importance of lipids?

A

a. Help absorb fat-soluble vitamins
b. Major fuel of hepatocytes and skeletal muscle
c. Phospholipids are essential in myelin sheaths and all cell membranes
d. Adipose tissue
i. Protective cushions around body organs
ii. Insulating layer beneath the skin
iii. Concentrated source of energy
e. Prostaglandins
i. Control of blood pressure
ii. Inflammation
f. Cholesterol – found in egg yolk, meats, organ meats, shellfish, and milk products
i. Stabilizes membranes
ii. Precursor of bile salts and all steroid hormones

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12
Q

How is cholesterol transported?

A

Cholesterol cannot dissolve in the watery blood plasma, so it must be carried around the body by transport proteins called lipoproteins.

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13
Q

Which cholesterol is considered good and bad? Why

A

a. Low density lipoprotein (LDL) is considered bad because it gets stuck in arteries
b. High density lipoprotein (HDL) is considered good because it doesn’t get stuck

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14
Q

How many proteins are there? How many are essential and nonessential?

A

a. 20 amino acids

b. 9 essential, 11 nonessential

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15
Q

What are complete proteins?

A

Complete proteins are those that contain all 9 essential amino acids in sufficient quantity – these are typically animal-based proteins

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16
Q

What are incomplete proteins?

A

Incomplete proteins are those that don’t contain all 9 essential amino acids, or don’t have sufficient quantities of them to meet the body’s needs, and must be supplemented with other proteins.

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17
Q

Describe the use of amino acids in the body (all or none rule, caloric intake)

A

a. All-or-none rule- All amino acids needed must be present for protein synthesis to occur
b. Adequacy of caloric intake- Protein will be used as fuel if there is insufficient carbohydrate or fat available

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18
Q

What is the importance of protein? (don’t memorize)

A

a. Structural materials: keratin, collagen, elastin, actin, myosin, microtubules, cilia, flagella, centrioles, etc.
b. Most are functional molecules: enzymes, receptors, transporter proteins, hemoglobin, antibodies, some hormones, transcription factors, etc.

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19
Q

What are vitamins and what is their function?

A

a. Organic molecules

b. Most function as coenzymes, making metabolism more efficient

20
Q

What are the two types of soluble vitamins?

A

a. Water-soluble vitamins
i. B complex and C are absorbed with water
ii. B12 absorption requires intrinsic factor
iii. Not stored in the body
b. Fat-soluble vitamins
i. A, D, E, and K are absorbed with lipid digestion products
ii. Stored in the body, except for vitamin K

21
Q

What are the seven essential minerals?

A

a. Calcium, phosphorus, potassium, sulfur, sodium, chloride, and magnesium
b. Others required in trace amounts

22
Q

What do minerals do?

A

Work with nutrients to ensure proper body functioning

23
Q

What is metabolism?

A

biochemical reactions inside cells involving nutrients

24
Q

What are two type of metabolic reactions?

A

a. Anabolism: synthesis of large molecules from small ones

b. Catabolism: hydrolysis of complex structures to simpler ones

25
Q

What is cellular respiration?

A

catabolism of food (& O2) fuels and capture of energy to form ATP in cells

26
Q

What is phosphorylation?

A

a. Enzymes shift high-energy phosphate groups of ATP to other molecules
b. Phosphorylated molecules are activated to perform cellular functions

27
Q

Describe the oxidation-reduction (Redox) reactions (OIL, RIG)

A

a. Oxidized substances have lost electrons

b. Reduced substances have gained electrons

28
Q

What is dehydrogenation?

A

often times, during redox, a proton (H+) accompanies the electron

29
Q

What do coenzymes (carriers) act as? What are their specific names?

A

a. Act as hydrogen (or electron) acceptors
b. Nicotinamide adenine dinucleotide (NAD+)
c. Flavin adenine dinucleotide (FAD)

30
Q

Describe the two mechanisms of ATP synthesis

A

a. Substrate-level phosphorylation (one stop shopping)

b. Oxidative phosphorylation

31
Q

Describe substrate-level phosphorylation

A

a. High-energy phosphate groups directly transferred from phosphorylated substrates to ADP
b. Occurs in glycolysis and the Krebs cycle

32
Q

Describe oxidative phosphorylation

A

a. Chemiosmosis- Couples the movement of hydrogen ions across mitochondrial membrane with phosphorylation of ADP to ATP
b. In the mitochondria
c. Carried out by electron transport proteins
d. Nutrient energy is used to create H+ gradient across mitochondrial membrane
e. Pairs of H+ flow through ATP synthase
f. Energy is captured and attaches phosphate groups to ADP

33
Q

Oxidation of glucose will give you how many ATP?

A

38 ATP

34
Q

Glucose is catabolized in which three pathways?

A

a. Glycolysis
b. (bridge)
c. Krebs’ cycle
d. Electron transport chain (ETC) and oxidative phosphorylation

35
Q

Where does glycolysis occur?

A

Cytoplasm of cell

36
Q

What are the steps of the glycolysis?

A

a. Sugar activation- Glucose is activated by phosphorylation and converted to 6-bisphosphate (2 ATP are lost)
b. Cleavage- 6-bisphosphate is cleaved into two 3-carbon fragments (pyruvate)
c. 4 ATP molecules are formed, and 2 NAD+ is reduced to 2 NADH + H+

37
Q

What are the final products of glycolysis?

A

a. 2 pyruvic acid
i. Converted to lactic acid if O2 not readily available
ii. Enter aerobic pathways if O2 is readily available
b. 2 NADH + H+ (reduced NAD+)
c. Net gain of 2 ATP

38
Q

Describe the transitional phase (bridge) to Kreb cycle

A

a. Each pyruvic acid is converted to acetyl CoA
b. Decarboxylation: removal of 1 C to produce acetic acid and CO2
c. Oxidation: H+ is removed from acetic acid and picked up by NAD+
d. Acetic acid + coenzyme A forms acetyl CoA

39
Q

Where does the Krebs’ Cycle occur and what is it fueled by?

A

a. Occurs in mitochondrial matrix

b. Fueled by pyruvic acid and fatty acids

40
Q

Describe the Krebs Cycle

A

a. Coenzyme A shuttles acetic acid to an enzyme of the Krebs cycle
b. Each acetic acid is decarboxylated and oxidized, generating:
i. 3 NADH + H+
ii. 1 FADH2
iii. 2 CO2
iv. 1 ATP
v. There are 2 acetic acids, so all of these are doubled

41
Q

What are 3 facts about the Krebs cycle?

A

a. Does not directly use O2
b. Breakdown products of fats and proteins can also enter the cycle
c. Cycle intermediates may be used as building materials for anabolic reactions

42
Q

Describe the electron transport chain and oxidative phosphorylation

A

a. The only part of metabolism that directly uses oxygen
b. Chain of proteins bound to metal cofactors on inner mitochondrial membrane
c. Coenzymes NADH and FADH2 deliver electrons and protons (they now are oxidized)
d. Electrons are shuttled along the inner mitochondrial membrane, losing energy at each step
e. Released energy is used to pump H+ into the intermembrane space
f. H+ diffuses back to the matrix in pairs via ATP synthase
g. ATP synthase uses released energy to make ATP
h. Electrons are delivered to O, forming O–
i. O– attracts H+ to form H2O

43
Q

Describe electron transport chain and oxidative phosphorylation (last step of ETC)

A

a. Electrons are delivered to O, forming O–

b. O– attracts H+ to form H2O

44
Q

Describe the electron transport chain and oxidative phosphorylation

A

a. Transfer of energy from NADH + H+ and FADH2 to oxygen releases large amounts of energy
b. This energy is released in a stepwise manner through the electron transport chain

45
Q

Describe the ATP synthase

A

a. Two major parts connected by a rod
b. Rotor in the inner mitochondrial membrane
c. Knob in the matrix
d. Works like an ion pump in reverse

46
Q

How many ATP are produced by each metabolic step?

A

a. Glycolysis: Net +2 ATP
b. Krebs Cycle: +2 ATP
c. ETC: +34 ATP
i. 10 NADH + H+ x 3 ATP= 30 ATP
ii. 2 FADH2 x 2 ATP= 4 ATP