Nutrient Digestion and Digestibility Flashcards

1
Q

what is nutrient digestibility

A
  • chemical analysis of feed provides information on nutrient composition
  • no information on digestibility in digestive tract (extent of digestion important
  • indication of bioavailability of nutrients (nutrients that is available for metabolism)
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2
Q

what is digestibility

A
  • the proportion of a feed that is not excreted in the feces and, therefore is assumed to have been absorbed
  • major determinant of nutritional value of feeds

digestible matter + undigestible matter = 100%

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3
Q

what is the concept of digestibility

A

high digestibility = high nutritional retention = high performance (positive for economics, less so for environment (manuer = nitrogen and phosphorus)
low digestibility = low nutrient rentention = poor performance

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4
Q

what are the methods of measure for feed digestibility

A
  • in vivo
  • in sotu
  • in vitro
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5
Q

what is in vivo

A
  • total fecal collection
  • indicator method
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6
Q

what is in situ

A
  • nylon bag
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7
Q

what is in vitro

A
  • tilley and terry method
  • gas prodcution
  • enzymes
  • regression using chemical analysis
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8
Q

what are step in vivo measurement of digestibility

A
  • test feed or diet given to animal in known amounts for several days - 4-14 adaption period (4-7 days to measure feed intake then collect feces)
  • 4-6 animals fed test or diet, digestibility differs; statistical analysis requires replication (consistent feed intake; time of feeding)
  • can use males of females
  • housed in metabolism crates or stalls, measure fecal output
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9
Q

why do you need an adaption period for feed

A
  • allow time for old feed to be totally excreted (so only test feed is in the diet)
  • might take animals GI a bit of time to adapt to the new feed - microorganisms
  • feed intake might go down = environmental adaptation to new feed
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10
Q

what are metabolism crates

A
  • sheep and pigs- adaptation periods
  • fecal collection - fecal bags or tray behind animal
  • urine - delivered by funnel into collection Jar below
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11
Q

how do you use digestibility for total fecal collection

A
  • determine DM % and nutrient content of test feed ( CP crude fat NDF ADF)
  • feed animal in metabolic crate, measure FI
  • collect total fecal output
  • determine DM % and nutrient content in feces
  • determine total DW or nutrient intake and total DM or nutrient output in feces
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12
Q

what is the difference between apparent and true digestibility

A
  • most measurements of digestibility are apparent nutrient digestibility
  • enzymes + sloughed-off epithelial cells + microbial cells in feces = endogenous losses
  • when we dont account for endogenous loss = apparent
  • when we do account for endogenous loss = true protein ( true should always be higher, because in apparent you over estimate protein)
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13
Q

what are indirect measurement of digestibility

A
  • some feed ingredients cannot be fed as sole feed ( 100% grain to a ruminant (rumen acidosis) or 100% fat to any animal)
  • measure digestibility indirectly using two digestibility trials
  • first, determine digestibility of a basal diet which can be fed at the 100% level (the only feed the animal is getting)
  • then combine the basal with the test ingredient and measure the digestibility of the basal diet and the test ingredient in combination
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14
Q

what is step 1 in indirect measurement of digestibility

A
  • determine digestibility of the basal diet
  • 60% digestibility of alfalfa
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15
Q

what is step 2 in indirect measurement of digestibility

A
  • determine the digestibility of 50/50 alfalfa/grain (5kg each on DM basis)
  • fecal output = 2.5 kg
  • partition the feces source
  • 5 kg of alfala = 2 kg of feces (calculation)
  • 10kg of feed = 2.5 feces by test
  • 5 kg of grain = 0.5 kg feces by difference
  • ## digestibility of grain
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16
Q

what are problems with total collection

A
  • need accurate estimate of feed intake (feed wastage, animals on pasture)
  • must collect all fecal output ( volume of material can be large =, tedious and labour-intesive)
17
Q

how do you measure digestibility with indicator method

A
  • mix an indigestible indicator into the feed at a low and known level (usually 0.5%) (with adataptation period
  • feed to an animal (no need to measure intake)
  • collect a grab sample of feces (no need to collect all)
  • measure concentration of indicator in feces
18
Q

what are the properties of ideal indicator or marker

A
  • cannot be digested or absorbed from GI
  • inert, no toxic metals or metabolic effects
  • intimately associated with material that it is to mark (the diet )
  • uniformly excreted in feces (grab samples)
  • stable
  • inexpensive
  • readily available
  • easily detected, easy to measure
19
Q

what is an example of an internal digestibility marker

A
  • indigestible NDF
  • lignin (good for browse)
  • acid insoluble ash ( silicates)
20
Q

what is an example of an external marker of digestibility

A
  • chromic oxide (Cr2O3) - chemically added
  • titanium oxide or ytterbium - more expensive/ more difficult
21
Q

what is an in vitro techniques and in situ technique for watching digestibiltiy

A
  • stimulate rumen fermentation and small intestinal (gastric digestion) under controlled conditions
  • test tube
22
Q

what is tilley and terry

A

artificial rumen

23
Q

what is a nylon bag

A

have the feed placed in a nylon bag and place it in the GI