Nucleic Acids Manipulation and Detection Methods

Question 1

Nucleases

Answer 1

Enzymes that cut DNA by breaking phosphoester bonds linking nucleotides

Question 2

Exonucleases

Answer 2

Nucleases that remove nucleotides one by one from the end of the DNA strand

Question 3

Exonuclease III

Answer 3

Degrades DNA from 3’ to 5’. Can create single strand DNA

Question 4

Endonucleases

Answer 4

Break internal bonds within DNA

Question 5

S1 nucleases

Answer 5

Only cleaves single strands

Question 6

DNase1

Answer 6

Cuts both single and double strands, can be used to locate sections protected by other proteins

Question 7

Restriction enzymes

Answer 7

Endonucleases that cut only after a specific base sequence

Question 8

Modifying enzymes

Answer 8

Add or remove specific groups

Question 9

Alkaline phosphatase

Answer 9

Remove phosphate from 5’ end of chain

Question 10

Polynucleotide kinase

Answer 10

Transfers phosphate from ATP to the 5’ end

Question 11

Terminal deoxy-nucleotidyl transferase

Answer 11

Adds nucleotides to the 3’ end of DNA strands

Question 12

Klenow fragments

Answer 12

Can remove 3’ overhangs and fill in 5’ ones.

Question 13

Agrose Gel Electrophoresis

Answer 13

A method for seperating nucleic acids

Question 14

Hybridization

Answer 14

The process of binding markes probes to genes of interest