Nucleic acids and ATP Flashcards
Definition of a genome
The number of different genes within an organism.
Defintion of a proteome
The number of different proteins the genome is able to code for.
Definition of a gene
Section of DNA on a chromosome coding for one or more polypeptide.
Definition of an allele
Variation of a gene.
What are the 3 components of a nucleotide?
- Phosphate group
- Pentose sugar (deoxyribose for DNA / ribose for RNA)
- Nitrogen-containing base: uracil (RNA), thymine (DNA), cytosine, guanine and adenine.
State the DNA nitrogen-containing bases (4 points).
Adenine
Thymine
Guanine
Cytosine
List the 4 RNA nitrogen-containing bases
Adenine
Uracil
Guanine
Cytosine
Label a diagram of a DNA polymer
*Refer to Nucleic acids and ATP new notes
7 structures and functions of DNA
- Sugar phosphate backbone: provides stability and protects bases to keep code sequence intact.
- Longer molecule: can store lots of information in DNA base sequence.
- Helix: compact shape.
- Double-stranded: semi-conservative replication with both strands acting as templates.
- Many weak hydrogen bonds between bases for replication: strong but can be unzipped by DNA helicase.
- Strands run in opposite directions- anti-parallel: bases face inwards for complementary base pairing, protects DNA base sequence.
- Complementary base pairing: accurate replication.
Describe the DNA in mitochondria and chloroplasts (2 points).
Where it is found in each organelle?
- Circular DNA.
- No histones- naked DNA.
Mitochondrial DNA is found in the matrix. Chloroplast DNA is found in the stroma.
Describe the endosymbiosis theory (2 points).
- Smaller prokaryotic cells being absorbed within a membrane by larger cells forming eukaryotic cells.
- Smaller and larger cells live together.
1 similarity and 7 differences between eukaryotic and prokaryotic DNA
- E.DNA is loacted in the nucleus; P.DNA is located in the cytoplasm.
- E.DNA is longer; P.DNA is horter.
- E.DNA has histones; P.DNA has no histsones- naked DNA.
- E.DNA has many non-coding/repetitive regions; P.DNA has some non-coding/repetitive regions.
- E.DNA has no plasmids; P.DNA has plasmids.
- E.DNA has linear DNA; P.DNA has circular DNA.
- E.DNA has hydrogen bonds between bases and phosphodiester bonds between nucelotides; P.DNA has hydrogen bonds between bases and phosphodiester bonds between nucleotides.
- E.DNA has deoxyribose sugar; P.DNA has ribose sugar.
What are the 4 functions of non-coding DNA?
- Promotor region
- Terminator region
- Enhancer region
- Silencer region
What is a promotor region?
RNA polymerase can bind during transcription.
What is a terminator region?
RNA polymerase released after transcription.
What is an enhancer region?
Allows proteins for transcription to bind.
What is a silencer region?
Allows proteins to bind to prevent transciption AVP.
Describe RNA
A single, shorter polynucloetide chain with ribose pentose sugar and the nitrogenous base: uracil instead of thymine.
Description and function of mRNA
Messenger RNA; transfers genetic information from DNA to ribosomes, made in transcription.
Description and function of rRNA
Ribosomal RNA; component of ribosomes, used in transcription.
Description and function of tRNA
Transfer RNA; used in translation.
Describe the structure of mRNA (4 points).
- Long and single-stranded.
- No hydrogen bonds.
- Complementary to the template DNA strand.
- Contains codons.
Defintion of codons
A sequence of three adjacent nueclotides in mRNA that codes for one amino acid.