Nucleic Acids Flashcards
What is the first amino acid in a protein translated from mitochondrial mRNA?
Formylmethionine (fMet).
What is chromatin?
DNA + associated proteins
What are nucleosomes?
DNA wrapped around a core of histone proteins - an example of chromatin.
Name the purines
Adenine and guanine.
Describe charges in DNA.
Phosphate groups (backbone) negative Histones positive
What direction is DNA synthesised?
5’ to 3’ of new strand. dNTP added to 3’ end of primer.
What is DNA primase?
An RNA polymerase which produces an RNA primer.
How is an incorrect nucleotide removed?
3’ to 5’ exonuclease activity of DNA polymerase.
Which is the antisense strand?
The template strand.
What does RNA polymerase II do?
Transcribes encoding mRNA.
What is the promoter gene?
The DNA sequence at which the transcription complex assembles.
How do transcription factors facilitate transcription?
They remodel chromatin. Hyperacetylation = gene expression. Hypoacetylation = gene repression. (They upregulate or downregulate the basal transcription complex).
For stability, what 2 features are added to the primary transcript (pre-mRNA)?
A 5’ cap - a modified nucleotide (7-methylguanosine)
A polyA tail, 11-30 bases downstream of AAUAAA.
What is alternative splicing?
Some exons are ignored, e.g. a mutate exon in dystrophin which causes DMD is ignored.
What is a tRNA with an amino acid attached called?
Charged