Nucleic

Question 1

Found in all living cells

Answer 1

Nucleic acids

Question 2

Two classes of nucleic acids

Answer 2

DNA and RNA

Question 3

Nucleic acids are ___ in cold water, ____ in alcohol but mixes instantly in ____

Answer 3

mildy soluble, insoluble and weak alkali metal salts

Question 4

Heating with ___ is essential

Answer 4

alkali (10% NaOH)

Question 5

How are nucleic acid separated?

Answer 5

Through acid extraction at pH 4-5

Question 6

Complete hydrolysis of RNA nucleotides yields

Answer 6

mixture of sugar (ribose), purine (adenine and uracil) or pyrimidine bases (guanine and cytosine), and phosphate

Question 7

Bases found in nucleic acids

Answer 7

Purine bases like adenine and guanine

Question 8

are purine bases responsible for storing and transmitting genetic information and protein synthesis

Answer 8

Adenine and guanine

Question 9

Formation of precipitate occurs due to the reaction between the ?

Answer 9

silver ions (Ag+)

Question 10

Difference of RNA AND DNA

Answer 10

RNA- presence of additional hydroxyl group (OH-) on the ribose sugar
DNA- contain deoxyribose as its sugar component that lacks OH- at the carbon 2’ position which makes it more resistant to acid hydrolysis

Question 11

Prolonged exposure of DNA to strong acids eventually lead to its ___?

Answer 11

Degradation

Question 12

What is the bases of DNA?

Answer 12

A,G,C,T

Question 13

What is the bases of RNA?

Answer 13

A,G,C,U

Question 14

Major steps in dna isolation and profiling

Answer 14

Collection of sample, cell lysis, dna purification, quantifiaction using spectrophometer, amplificatioon, fragmentation, separation, detection and analysis, and data interpretation