Normal Lymphopoiesis

Question 1

normal location of B and T cells in lymph node

Answer 1

B cells = cortex

T cells = paracortex

Question 2

normal B cell kappa:lambda ratio

Answer 2

2 kappa: 1 lambda

Question 3

surface molecules that mark B cells

Answer 3

CD 79, 10, 20, 22
TdT
Bruton tyrosine kinase

Question 4

somatic hypermutation

Answer 4

rapid division that happens to B cells happens in the secondary lymphoid tissue germinal center until a high affinity antibody producing B cell results!

Question 5

plasma cell surface markers

Answer 5

CD38, CD138

Question 6

what kind of biopsy of lymph nodes should you order?

Answer 6

EXCISIONAL BIOPSY - so you can see architecture

Question 7

reactive hyperplasmia and pathology

Answer 7

follicular hyperplasia - due to bacterial infection
interfollicular hyperplasia - viral infections
sinus histiocytosis: expansion of medullary sinus histiocytes

Question 8

normal immunophenotype of follicles

Answer 8

CD20, CD2, BCL2

Question 9

normal immunophenotype of germinal center

Answer 9

CD20, CD3, CD10, neg BCL2

Question 10

B symptoms

Answer 10

common in diseases of lymph nodes

fever, weight loss, night sweats

Question 11

t(14,18)

Answer 11

follicular lymphoma

Question 12

t(8,14)

Answer 12

Burkitt’s lymphoma

Question 13

B cell lymphoma of follicular origin - markers

Answer 13

monoclonal light chain

pos: CD19. CD20, CD10
negative: CD5

Question 14

B cell small lymphocytic lymphoma

Answer 14

monoclonal light chain

pos: CD19, CD20, CD5, CD23
negative: CD10