Non enteric Flashcards
Non glucose fermenter Vibrio sp.
Vibrio metschnikovii
Vibrio flagella
Monotrichous flagella (Shooting star motility)
Strring test reagent
0.5% sodium deoxycholate
Vibriostatic agent 0/129
2,4 diamino-6,7 diisopropyl pteridine
Vibriostatic disk
150 ug
V. cholerae serotypes 01
Ogawa (A,B) - India
Inaba (A,C) - Philipppines
Hikojima (A,B,C) - Japan
Vibrio spp. optimum temperature for growth
42C
responsible for the massive
amount of water and electrolytes loss
Cholera toxin or choleragen
oxidase negative Vibrio spp.
Vibrio metschnikovii
Lactose fermenter Vibrio sp.
V. vulnificus
High-salt mannitol medium
Wagatsuma agar
What vibrio sp. shows Kanagawa phenomenon
V. parahaemolyticus
Gram negative Oxidase positive Nitrate Reduction Motile Non Inositol Fermenter Vibriostatic agent 0/129 susceptible Facultative anaerobe
Vibrio spp.
String test positive vibrio sp.
V. cholerae
Antimicrobial agent for diarrhea
doxycycline (+azithromycin & ciprofloxacin if resistant)
Primary cause of summer diarrhea in Japan
V. parahaemolyticus
Strict halophile vibrio sp.
V. alginolyticus
Holding medium appropriate for Vibrio and Campylobacter
Cary-Blair to prevent dessication
Not recommended transport/holding medium for Vibrio and enteric Campylobacter
Buffered glycerol saline
Viable specimen up to 5 weeks (Vibrio spp.)
Strips of blotting paper soaked in liquid stol and placed in airtight plastic bags with a few drops of saline to maintain moisture
Recommended selective differential media for Vibrio spp.
Thiosulfate Citrate Bile Salt agar
Smooth, opaque, and iridescent with a greenish hue in SBA or chocolate agar
Vibrio spp.
Non sucrose fermenter vibrio spp.
V. parahaemolyticus
most V. vulnificus strains
Vibrio spp. that doesn’t grow on TCBS
Grimontia hollisae (formerly vibrio)
Gram negative Oxidase positive Indole positive Motile Non inositol fermenter Vibriostatic agent 0/129 resistant Facultative anaerobe
Aeromonas
Aeromonas mesophilic group
A. hydrophila
A. veronii
A. cavaiae
Aeromonas psychrophilic group
A. salmonicida
Traveler’s diarrhea (similar to enterotoxigenic E. coli)
Nebulous syndrome
Lactose fermenter aeromonas sp.
A. caviae
SBA B-hemolysis Aeromonas spp.
A. hydrophila
A. veronii biovar sobria
A. jandaei
Large, round, raised, opaque colonies with an entire edge and a smooth, often mucoid surface
Aeromonas
White to buff-colored pigmentation
Aeromonas
Pink centered colonies in CIN medium from mannitol fermentation with an uneven, clear apron resembling yersinia enterocolotica
Aeromonas
Gram negative Oxidase positive Motile Inositol fermenter Vibriostatic agent 0/129 susceptible Facultative anaerobe
Plesiomonas
Shiny, opaque, nonhemolytic colonies with a slightly raised center and a smooth, entire edge
Plesiomonas
Enhances the isolation of plesiomonads
Inositol brilliant green bile agar
Opaque (non-mannitol fermenting) colonies with an opaque apron in CIN medium
Plesiomonas
Differentiation of Enteric bacteria from non enteric bacteria
Enterobacteriaceae is oxidase negative while non enteric bateria is oxidase positive
Gram negative Capnophilic Motile Sodium Hippurate Hydrolysis positive Urease negative Microaerophilic (5% 02)
Campylobacter
Plesiomonas flagella
Monotrichous or 2-5 lophotrichous flagella
Campylobacter flagella
One polar flagellum
Motility on hanging drop preparations or when visualized under phase contrast microscopy
Causes abortion in domestic animals, zoonotic organisms
Campylobacter
Associated with Guillain-Barre Syndrome
Campylobacter
Most common cause of bacterial gastroenteritis
Campylobacter jejuni
Optimal growth for C. jejuni and other enteric campylobacters
42C
Recovered from biopsy materials
H. pylori
Transport medium of H. pylori
Stuart medium
for tissue samples: cysteine-brucella broth with 20% glycerol frozen at -70C
Moist, runny looking and spreading, usually non hemolytic; some are round and raised, others may be flat
C. jejuni and other enteric campylobacters
Smooth, convex, translucent colonies
C. fetus subsp. fetus
Dirty yellow pigment
C. mucosalis & C. hyointestinalis
Non invasive indirect test for campylobacter
urea breath test
