Non enteric

Question 1

Non glucose fermenter Vibrio sp.

Answer 1

Vibrio metschnikovii

Question 2

Vibrio flagella

Answer 2

Monotrichous flagella (Shooting star motility)

Question 3

Strring test reagent

Answer 3

0.5% sodium deoxycholate

Question 4

Vibriostatic agent 0/129

Answer 4

2,4 diamino-6,7 diisopropyl pteridine

Question 5

Vibriostatic disk

Answer 5

150 ug

Question 6

V. cholerae serotypes 01

Answer 6

Ogawa (A,B) - India
Inaba (A,C) - Philipppines
Hikojima (A,B,C) - Japan

Question 7

Vibrio spp. optimum temperature for growth

Answer 7

42C

Question 8

responsible for the massive

amount of water and electrolytes loss

Answer 8

Cholera toxin or choleragen

Question 9

oxidase negative Vibrio spp.

Answer 9

Vibrio metschnikovii

Question 10

Lactose fermenter Vibrio sp.

Answer 10

V. vulnificus

Question 11

High-salt mannitol medium

Answer 11

Wagatsuma agar

Question 12

What vibrio sp. shows Kanagawa phenomenon

Answer 12

V. parahaemolyticus

Question 13

Gram negative
Oxidase positive
Nitrate Reduction
Motile
Non Inositol Fermenter
Vibriostatic agent 0/129 susceptible
Facultative anaerobe

Answer 13

Vibrio spp.

Question 14

String test positive vibrio sp.

Answer 14

V. cholerae

Question 15

Antimicrobial agent for diarrhea

Answer 15

doxycycline (+azithromycin & ciprofloxacin if resistant)

Question 16

Primary cause of summer diarrhea in Japan

Answer 16

V. parahaemolyticus

Question 17

Strict halophile vibrio sp.

Answer 17

V. alginolyticus

Question 18

Holding medium appropriate for Vibrio and Campylobacter

Answer 18

Cary-Blair to prevent dessication

Question 19

Not recommended transport/holding medium for Vibrio and enteric Campylobacter

Answer 19

Buffered glycerol saline

Question 20

Viable specimen up to 5 weeks (Vibrio spp.)

Answer 20

Strips of blotting paper soaked in liquid stol and placed in airtight plastic bags with a few drops of saline to maintain moisture

Question 21

Recommended selective differential media for Vibrio spp.

Answer 21

Thiosulfate Citrate Bile Salt agar

Question 22

Smooth, opaque, and iridescent with a greenish hue in SBA or chocolate agar

Answer 22

Vibrio spp.

Question 23

Non sucrose fermenter vibrio spp.

Answer 23

V. parahaemolyticus

most V. vulnificus strains

Question 24

Vibrio spp. that doesn’t grow on TCBS

Answer 24

Grimontia hollisae (formerly vibrio)

Question 25

``` Gram negative Oxidase positive Indole positive Motile Non inositol fermenter Vibriostatic agent 0/129 resistant Facultative anaerobe ```

Answer 25

Aeromonas

Question 26

Aeromonas mesophilic group

Answer 26

A. hydrophila A. veronii A. cavaiae

Question 27

Aeromonas psychrophilic group

Answer 27

A. salmonicida

Question 28

Traveler's diarrhea (similar to enterotoxigenic E. coli)

Answer 28

Nebulous syndrome

Question 29

Lactose fermenter aeromonas sp.

Answer 29

A. caviae

Question 30

SBA B-hemolysis Aeromonas spp.

Answer 30

A. hydrophila A. veronii biovar sobria A. jandaei

Question 31

Large, round, raised, opaque colonies with an entire edge and a smooth, often mucoid surface

Answer 31

Aeromonas

Question 32

White to buff-colored pigmentation

Answer 32

Aeromonas

Question 33

Pink centered colonies in CIN medium from mannitol fermentation with an uneven, clear apron resembling yersinia enterocolotica

Answer 33

Aeromonas

Question 34

``` Gram negative Oxidase positive Motile Inositol fermenter Vibriostatic agent 0/129 susceptible Facultative anaerobe ```

Answer 34

Plesiomonas

Question 35

Shiny, opaque, nonhemolytic colonies with a slightly raised center and a smooth, entire edge

Answer 35

Plesiomonas

Question 36

Enhances the isolation of plesiomonads

Answer 36

Inositol brilliant green bile agar

Question 37

Opaque (non-mannitol fermenting) colonies with an opaque apron in CIN medium

Answer 37

Plesiomonas

Question 38

Differentiation of Enteric bacteria from non enteric bacteria

Answer 38

Enterobacteriaceae is oxidase negative while non enteric bateria is oxidase positive

Question 39

``` Gram negative Capnophilic Motile Sodium Hippurate Hydrolysis positive Urease negative Microaerophilic (5% 02) ```

Answer 39

Campylobacter

Question 40

Plesiomonas flagella

Answer 40

Monotrichous or 2-5 lophotrichous flagella

Question 41

Campylobacter flagella

Answer 41

One polar flagellum | Motility on hanging drop preparations or when visualized under phase contrast microscopy

Question 42

Causes abortion in domestic animals, zoonotic organisms

Answer 42

Campylobacter

Question 43

Associated with Guillain-Barre Syndrome

Answer 43

Campylobacter

Question 44

Most common cause of bacterial gastroenteritis

Answer 44

Campylobacter jejuni

Question 45

Optimal growth for C. jejuni and other enteric campylobacters

Answer 45

42C

Question 46

Recovered from biopsy materials

Answer 46

H. pylori

Question 47

Transport medium of H. pylori

Answer 47

Stuart medium | for tissue samples: cysteine-brucella broth with 20% glycerol frozen at -70C

Question 48

Moist, runny looking and spreading, usually non hemolytic; some are round and raised, others may be flat

Answer 48

C. jejuni and other enteric campylobacters

Question 49

Smooth, convex, translucent colonies

Answer 49

C. fetus subsp. fetus

Question 50

Dirty yellow pigment

Answer 50

C. mucosalis & C. hyointestinalis

Question 51

Non invasive indirect test for campylobacter

Answer 51

urea breath test