NGS

Question 1

True or false: during Illumina sequencing, all four capped fluorophore nucleotides are added together at every cycling.

Answer 1

True

Question 2

What type of sequencing is being illustrated here?

Answer 2

Illumina

Question 3

Portable on-site environmental sequencing can be undertaken by:

Answer 3

Oxford Nanopore sequencing

Question 4

True or false: the Illumina Adapter referred to as an ‘index sequence’ is the equivalent of what is normally referred to as a ‘barcode’.

Answer 4

True

Question 5

The two strands represented in blue are:

Answer 5

Complementary strands

Question 6

The oligonucleotides are attached to the inner surface of Illumina flow cells are of two types. Collectively, these oligos are referred to as:

Answer 6

Capture oligos

Question 7

What is the purpose of the component, a few of which have been encircled in red?

Answer 7

Blocking agent, to prevent DNA synthesis

Question 8

True or false: bridge amplification is a form of PCR amplification.

Answer 8

True

Question 9

The red arrow points towards A, while the green arrow points towards B, meaning:

Answer 9

A is the original strand; B is the newly-synthesised strand