Bacterial Replication

Question 1

In bacterial DNA replication, most of the DNA is synthesised by the enzyme ____ ___________ __.

Answer 1

DNA polymerase III

Question 2

The gaps between Okazaki fragments are:

Answer 2

Filled in by DNA polymerase I

Question 3

Bacterial chromosomes are usually circular, and replication therefore proceeds in:

Answer 3

Two directions around the circle

Question 4

True or false: the DNA lagging strand is made entirely using DNA polymerase I.

Answer 4

False

Question 5

True or false: a lagging strand of DNA is synthesised discontinuously.

Answer 5

True

Question 6

DNA gyrase:

Answer 6

Untwists strands of DNA

Question 7

Single-stranded binding protein:

Answer 7

Keeps two complementary strands of DNA separated

Question 8

Primase:

Answer 8

Is an RNA polymerase used to synthesise short RNA primers

Question 9

DNA ligase:

Answer 9

Links the 3’-OH to the 5’-PO4, creating the phosphodiester bridge

Question 10

DNA polymerase:

Answer 10

Progressively adds a complementary nucleotide to the 3’ end of a new strand of DNA

Question 11

During replication, DNA gyrase:

Answer 11

Removes the supercoiling

Question 12

True or false: in bacterial DNA synthesis, the lagging strand is made five minutes after the leading strand.

Answer 12

False

Question 13

Natural primers are made of:

Answer 13

Single-stranded RNA