Bacterial Transcription Flashcards
RNA polymerase synthesises:
An RNA copy of the gene, using the antisense strand as a template
An example of an allosteric protein is:
The lac repressor
For which nitrogenous base does DNA substitute thymine?
Uracil
The process of making an RNA copy of a gene is:
Transcription
When cAMP levels are high in a cell:
Glucose levels are low
In bacterial genomes, the ‘minus 10’ region is:
Ten nucleotides upstream of a gene
Hairpin loop structures are found in:
mRNA
The operon is kept off in the presence of glucose by:
In the presence of glucose, cAMP is low. Thus, cAMP will not bind to CRP. CRP will not bind to region b, the CRP site, which is needed for RNA polymerase to bind to the promoter.
Inverted repeats make a:
Single-stranded loop and a double-stranded stem
When given a choice of sugars to metabolise, E. coli will:
Preferentially break down glucose first
The direction of synthesis is:
5’ to 3’ for both DNA and RNA
True or false: mRNA has a base sequence which is complementary to the anticoding strand of DNA.
False
A consensus sequence found in a bacterial promotor gene is:
An idealised sequence, which shares much sequence similarity for many genes
Transcription begins when:
RNA polymerase recognises a promotor sequence, binds to the DNA, and synthesises RNA from the 5’ end to the 3’ end of the new piece.
A high level of transcription can be achieved from the lac operon under the following conditions:
Low glucose, high cAMP, high lactose