Next Gen Sequencing

Question 1

Genomics definition

Answer 1

the systematic study on a whole-genome scale for the identification of genetic contributions to human conditions

Question 2

Requirements of sanger capillary sequencing

Answer 2

Requires DNA template, primer, polymerase, dNTPs, and fluorescently/radioactively labeled ddNTPs

Question 3

Limitations of sanger capillary sequencing

Answer 3

1) Limited to 300-1000 bases

2) £300K (in 2004) for 100 capillary sequencer

Question 4

Hierarchical Shotgun Sequencing

Answer 4

Longer sequences are subdivided into smaller fragments that can be sequenced separately, and subsequently they are re-assembled to give the overall sequence