Exam questions Flashcards
What is the main application of quantitative trait loci data?
(a) used to locate deleterious mutant genes
(b) genomic mapping in individuals in natural populations
(c) statistically identify single gene trait loci
(d) identify genes associated with some phenotypic trait
(e) to develop gene therapy methods
d) identify genes associated with some phenotypic trait
What is the main goal of the candidate locus approach?
(a) to identify human pathogenic genes
(b) to develop vaccines
(c) to optimize genetic variation with athletic sports training
(d) none of the answers
(e) all of the answers
a) to identify human pathogenic genes
What is the ideal length for a PCR primer?
(a) 10-18 bases in length.
(b) 18-40 bases in length.
(c) 41-60 bases in length.
(d) 60-80 bases in length.
(e) 80-100 bases in length.
b) 18-40 bases in length
A transversion mutation will exchange guanine for:
(a) cytosine;
(b) another purine;
(c) a pyrimidine;
(d) either a purine or a pyrimidine;
(e) (a) and (c).
c) a pyrimidine; either cytosine or Thymine
A nonsense mutation is one in which:
(a) the codon for one amino acid is changed to that for another;
(b) the codon for one amino acid is changed to another codon for the
same amino acid;
(c) a codon is removed;
(d) a codon is replaced with an anticodon;
(e) the codon for an amino acid is changed to a termination codon.
e) the codon for an amino acid is changed to a termination codon.
Which of the following applies to AFLP markers?
(a) amplify random loci about the genome
(b) easy to score heterozygotes
(c) consist of an array of repeating nucleotides
(d) they are co-dominant markers
(e) all of the above
d) They are co-dominant markers
When carrying out Sanger sequencing what reagent is added to ensure that the DNA remains single-stranded before capillary electrophoresis?
(a) 95% ethanol.
(b) 70% ethanol.
(c) Formamide.
(d) Isopropanol.
(e) All of the above.
c) Formamide
Which of the following is NOT a mutagen that functions as a base modifying agent?
(a) Nitrous acid;
(b) Hydroxylamine;
(c) N-methyl-N’-nitro-N-nitrosoguanidine (MNNG);
(d) (a) and (c);
(e) Ethidium bromide.
Ethidium Bromide
Ethidium Bromide is an intercalating agent that binds with DNA not a base modifying agent.
Remember to read the question clearly.
Which of the following is NOT true for UV light?
(a) used in the laboratory to induce mutations;
(b) causes adjacent thymines to dimerise;
(c) leads to helix distortion;
(d) causes strand or chromosome breaks;
(e) has a maximum effect at 260nm.
(e) has a maximum effect at 260nm.
260nm is the lowest wavelength used in the calculation of DNA purity (the other being 280nm
Which of the following repair mechanisms is caused by a second mutation on a different gene?
(a) SOS response;
(b) intergenic suppression;
(c) intragenic suppression;
(d) deoxydipyrimidine photolyase;
(e) glycosylase.
(b) intergenic suppression;
Intergenic (also known as extragenic) suppression relieves the effects of a mutation in one gene by a mutation somewhere else within the genome. The second mutation is not on the same gene as the original mutation.
Which of the following repair mechanisms leaves an apurinic or apyrimidinic (AP) site? (a) SOS response; (b) intergenic suppression; (c) intragenic suppression; (d) deoxydipyrimidine photolyase; (e) glycosylase.
(e) glycosylase.
Which of the following have been successfully used to type strains of
Mycobacterium tuberculosis?
(a) agglutination serotyping, RFLP analysis and spoligotyping;
(b) agglutination serotyping, RFLP analysis and mixed linker PCR;
(c) plasmid typing, mixed linker PCR and spoligotyping;
(d) RFLP analysis, mixed linker PCR and spoligotyping.
(e) plasmid typing, RFLP analysis and spoligotyping;
(d) RFLP analysis, mixed linker PCR and spoligotyping.
In relation to the use of detection techniques to identify mycobacteria which of the following statements is INCORRECT?
(a) The acid fast test cannot determine individual species.
(b) The probe CM3, used at high stringency, only hybridises to
Mycobacterium tuberculosis-complex DNA.
(c) Mycobacterial rRNA probes bind to multiple sites in the mycobacterial
DNA thus increasing sensitivity.
(d) The CM3 polymerase chain reaction system can be used to specifically
detect Mycobacterium tuberculosis.
(e) Use of sequence capture polymerase chain reaction leads to a 10-100
fold increase in sensitivity.
(c) Mycobacterial rRNA probes bind to multiple sites in the mycobacterial DNA thus increasing sensitivity.
Southern blotting is a form of nucleic acid analysis that uses:
(a) DNA samples and DNA probes;
(b) DNA samples and RNA probes;
(c) RNA samples and DNA probes;
(d) RNA samples and RNA probes;
(e) protein samples and DNA probes.
(a) DNA samples and DNA probes;
though RNA probes can be used
The actual transfer process in the technique of Southern blotting is:
(a) the transfer of protein to a membrane;
(b) the transfer of an antibody to a membrane;
(c) the transfer of RNA to a membrane;
(d) the transfer of DNA to a membrane;
(e) the transfer of chromosomes to a membrane.
(d) the transfer of DNA to a membrane;
Red safe is used during the electrophoresis process because:
(a) it assists the migration of DNA fragments;
(b) it binds to the DNA and fluoresces when exposed to UV radiation;
(c) it binds to the DNA and fluoresces in the dark;
(d) it retards the migration of DNA fragments;
(e) it digests DNA so fragments can be seen.
(b) it binds to the DNA and fluoresces when exposed to UV radiation;
HindIII restriction enzyme acts on plasmid DNA by:
(a) cutting single strand DNA at the promoter sites;
(b) cutting single strand DNA at specific sites;
(c) cutting double strand DNA at the promoter sites;
(d) cutting double strand DNA at specific sites;
(e) cutting any DNA strand at a random number of sites.
(d) cutting double strand DNA at specific sites;
Promoter sites are the sites at which polymerase initially binds.
If the plasmid MMU2 was cut with BamHI and there were two cutting sites, the
number of fragments seen on a red safe -stained agarose gel would be:
(a) 5;
(b) 4;
(c) 3;
(d) 2;
(e) 1.
(d) 2