Introns and Exons Flashcards
Characteristics of Prokaryotic cells: (7)
• No membrane bound organelles • Simple genome organization • Genes usually do not have introns • Between 500 and 4000 genes – M. genitalium has 477 genes • Great degree of diversity • Unicellular • Circular DNA
Characteristics of Eukaryotic Cells (9)
- All organelles are membrane-bound (e.g. nucleus)
- Complex genome organization
- Large genome size
- Between 6,000 and 30,000 genes
- Genes have introns (non-coding regions)
- Large amount of regulatory DNA - to control gene expression
- High degree of genetic redundancy
- Unicellular or multicellular
- Linear DNA
Proteomics
the large-scale study of proteins that
are produced in a cell using the genome - particularly their structures and functions.
Transcriptomics
analysis of transcriptomes (gene expression patterns)
transcriptomes are dynamic – patterns vary with cell type, developmental stage etc.
Central dogma of molecular biology
DNA to RNA to Protein
You cannot go Protein to RNA to DNA though you can go RNA to DNA.
Process of gene expression
1) transcription - RNA is transcribed from DNA template
2) RNA processing - RNA transcript is spliced and modified to produced mRNA which moves from the nucleus to the cytoplasm
3) Amino acid activation - each amino acid attaches to its proper tRNA via specific enzymes and ATP
4) Translation - tRNA add the amino acids to the polypeptide chain as the mRNA is moved through the ribosome one codon at a time.
Steps in transcription
1) initiation - RNa polymerase binds to promoter (starting point) and initiates RNA at the start point of the template strand
2) Elongation - polymerase moves downstream from 5’ to 3’ creating the RNa strand
3) Termination - upon transcribing the terminator at the end of the sequence the RNA polymerase detaches from the DNA and the RNA transcript is released.
Difference in transcription between prokaryotes and eukaryotes
• In prokaryotes – the RNA transcript of a protein-coding gene is immediately useable as mRNA
• However, in eukaryotic cells it must first
undergo processing
RNA processing
1) a cap (modified guanosine triphosphate) is added to the 5’ end
2) Introns are excised and the exons spliced together
3) 50 to 250 adenine nucleotides are added to the 3’ (called a Ploy (A) tail)
Chromatin
The complex combination of DNA, RNA and protein that makes up eukaryotic chromosomes
Euchromatin
a lightly packed form of chromatin - that is rich in gene concentration,
and is often (but not always) under active transcription
Heterochromatin
a tightly packed form of DNA - little or no gene expression in heterochromatic regions of the genome
Role of exons in protein domains
Different exons encode separate domains of the protein product. Each domain, an independantly folding part of the protein performs a different function in the cell.
What the pressence of exons and introns means:
The presence of exons and introns means that new proteins can evolve by exon shuffling among genes and therefore the potential to form new proteins with novel functions.
Control of gene expression
• The control of gene expression enables
individuals to adjust their metabolism to changes in their external and internal
environment and to carry out each of the functions of the cell only when needed.
• The enzymes that transcribe DNA must locate
the right genes at the right time – otherwise serious imbalances and diseases may occur