nematodes Flashcards

1
Q

phylum:
class:

A

phylum: nemathelminthes
class: nematoda

intestina spp: [NAS]
Necator americanus
Anclyostoma duodenale
Strongloides sterocoralis

intestinal-tissue spp: [TD]
Trichinella spiralis
Dracunculus medinensis

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2
Q

The feeding, non-infective first-stage larval form of certain nematodes, characterized by a short buccal cavity and a prominent genital primordium.

A

rhabditiform larvae
[can feed but cannot infect
have a short mouth area and a developing reproductive system.]

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3
Q

The non-feeding, infective third-stage larval form of nematodes, adapted for host penetration with a long esophagus and a pointed or notched tail.

A

Filariform Larvae
[do not eat but are ready to infect]

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4
Q

A flared, cuticular expansion at the posterior end of male nematodes, used for grasping the female during mating.

A

Copulatory Bursa

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5
Q

The oral opening of nematodes, which varies in length and structure and is used for feeding and host attachment.

A

Buccal Cavity

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5
Q

A form of asexual reproduction where a female produces offspring without fertilization by a male

A

parthenogenetic
*seen in Strongyloides stercoralis

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5
Q

reproductive strategy where the female gives birth to live larvae instead of laying eggs.

A

viviparous
*observed in Trichinella spiralis.

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6
Q

A reproductive strategy where the female produces eggs that hatch inside her body, releasing live larvae.

A

ovoviviparous
*as seen in Dracunculus medinensis.

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7
Q

A term referring to a fertilized female nematode carrying developing eggs or larvae.

A

gravid
*such as the gravid T. spiralis female that releases live larvae
[means a female worm is carrying fertilized eggs or developing larvae inside her body.]

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8
Q

The process by which a larva forms a protective cyst within host tissue, allowing for long-term survival and transmission.

A

encystation
*as seen in Trichinella spiralis.

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9
Q

general characteristics of nematodes

A

● Multicellular roundworms (cylindrical in cross-section).
● Bilaterally symmetrical, with a complete digestive and reproductive system.
● Nematodes are eukaryotes/ eukaryotic cells.

[Multicellular roundworms:
made up of many cells, and their bodies are shaped like tubes (cylindrical).
Bilaterally symmetrical:
If you cut them down the middle, both sides of their body would look the same. They also have a full digestive and reproductive system.
Eukaryotic:
Their cells have a nucleus.]

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10
Q

what are the 3 morphologic forms of nematodes

A
  1. Eggs
    Female sex cells post-fertilization, varying in size & shape.
  2. Juvenile worms (larvae)
    Long, slender, and actively developing.
  3. Adult worms
    Mature nematodes, with
    separate sexes (Dioecious).
    Females are larger than males.
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11
Q

it refers to a species that has separate male and female individuals

A

Dioecious
[means there are distinct male and female worms, rather than both sexes being in the same body]

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12
Q

when does the nematode reproductive system begins

A

when fertilized eggs are expelled via stool

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13
Q

briefly explain the maturation process

A

Eggs → Larvae (juveniles) → Adult worms

○ Developing larvae emerge from fertilized eggs in the right environment. [ environment is suitable (like warm, moist conditions), baby worms (larvae) break out of the fertilized eggs.]
○ Adult worms reside in the intestine, focusing on nutrition & reproduction.

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14
Q

briefly explain how fertilized adult female nematodes lay eggs in the intestine

A

eggs exit via stool and require warm, moist soil (2-4 weeks) for development.
once mature, the eggs become infectious and restart the cycle

[-cycle starts with fertilized eggs being released in stool then develop in the environment and eventually become infectious.
-person or animal accidentally swallow these infectious eggs
-once inside the intestine, the eggs hatch into larvae.
-larvae mature into adult worms, which reproduce and lay more eggs = restarting the cycle.

Key Point:
The infective stage is usually the egg, not the larvae (for most nematodes). The larvae only develop inside the host after the eggs are swallowed.]

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14
Q

where does the Trichinella & Dracunculus primarily reside in

A

tissue, not js the intestine
*Some nematodes can exist free-living, independent of a host.

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15
Q

how nematodes can infect humans

A
  1. ingestion
    Consuming infective eggs or larvae
    (e.g., Pinworms, Ascaris )
  2. inhalation
    Eggs inhaled and swallowed.
    (e.g., Enterobius)
  3. skin penetration
    Infective larvae burrow into the skin
    (e.g., Hookworms)
  4. vector-borne transmission
    Blood-sucking insects transmit filarial worms
    (e.g., Wuchereria Bancrofti)
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16
Q

habitat of adult nematode

A
  1. small intestine
    ASCARIS LUMRICOIDES
    STRONGYLOIDES STERCORALIS
    HOOKWORM
    TRICHINELLA SPIRALIS
  2. large intestine
    TRICHURIS TRICHIURA
    ENTEROBIUS VERMICULARIS
  3. lymphatic
    WUCHERIA BANCROFTI
    BRUGIA MALAYI
  4. subcutaneous
    LOA LOA
    ONCHOCERCE VOLVULUS
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17
Q

what are the hookworm adult that can be found in the small intestine

A

[CASHT]
Capillaria philippinensis
Ascaris lumbricoides
Strongyloides stercoralis
Hookworm
Trichinella spiralis

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18
Q

what are the 2 organisms of hookworm and briefly explain its
1.shape
2. buccal capsule (mouth parts)
3. borsa and dorsal ray
4. spicules

A

Ancylostoma duodenale
Necator americanus.

  1. both have a curve or hook-like appearance
  2. A: teeth
    N: cutting plates
  3. copulatory bursa in males used for matting. (show subtle structural diff in dorsal ray)
  4. male reproductive structures also differ in shape and orientation btwn the 2
19
Q

why does the nematode eggs has thin shell

A

as it contains developing embryos
- to hold and protect

20
Q

morphology of hookworms

A

Ancyclostoma duodenale
Necator americanus

both have
- thin, smooth, colorless shell
- unsegmented or embryonic cleavage
= they are morphologically indistinguishable, report as hookworm ova

*N (60-75um) is bigger than A (55-60um)

21
Q

common name of

A

Ancyclostoma duodenale:
Old World Hookworm
*clear spaces btwn segmented ovum and thin clear shell of A duodenale ova
[The egg has a thin, clear outer shell that is smooth.
Inside the shell, the developing embryo (segmented ovum) has a clear space around it = does not completely fill the egg.]

Necator americanus:
New World Hookworm

22
differentiation btwn eggs of Necator spp. and Ancylostoma spp.
difficult and impractical - features of an hookworm: ovaluster of eggs inside and ovoidal pouch, with very thin hyaline shell
23
larval stages in hookworms
rhabditiform larvae (feeing): 270 (newborn)-700(5days)um long buccal cavity small genital primordium *thicker and shorter in shape *blunt tail *has short buccal cavity filariform larvae (infective): infective stage after second molt slender, short esophagus distinct pointed tail (marking it as the infective form of penetrating the host skin) *slender and longer in shape *pointed tail *has long buccal cavity *THEY ARE USUALLY GROWING IN THE ENVIRONMENT
23
what does it mean when the filariform larvae has a pointed tail
means the infective form capable of penetrating the host skin
24
Ancyclostoma duodenale Necator americanus - size in female and male - copulatory bursa (male) - buccal capsule - color - distinctive feature
size in female and male: female is bigger than male copulatory bursa (male): present, umbrella-like structure buccal capsule: Ancyclostoma duodenale - actual teeth [2 PAIRS] Necator americanus - pair of cutting plates [SEMILUNAR CUTTING PLATES] color: grayish-white to pink distinctive feature: Ancyclostoma duodenale - less pronounced hook Necator americanus - pronounce hook at anterior end
25
hookworm - MOT - diagnostic stage - infective stage
MOT: skin penetration Diagnostic stage: hookworm ova Infective stage: filariform larvae
25
life cycle of hookworm
1. Eggs in Feces: Infected humans excrete eggs through feces. 2. Larval Development in Soil: Eggs hatch into rhabditiform larvae (L1) in the soil, which then moult into L2. 3. Infective Stage: L2 moults into filariform larvae (L3), which is the infective stage. [within 24-48 hours, the larvae undergo 2 molds from L1 to L2 to L3] 4. Entry into Host: L3 larvae penetrate the skin, enter the circulation, and travel to the heart and lungs. [reach to the lungs where thy penetrate capillaries then migrate to alveoli > ascend to bronchioles and coughed up to swallow again to enter the small intestine] 5. Migration to Intestines: In the lungs, larvae rupture into the alveolar space, ascend through the trachea and pharynx, and are swallowed. 6. Maturation in Intestines: The larvae develop into adult worms in the small intestine within 1–2 months. 7. Egg Deposition: The adult female lays eggs, which are passed out in feces, continuing the cycle. [10,000-20,000]
26
lifespan of Necator: Anclyostoma:
Necator : over 4 years Ancylostoma : 2-7 years
27
what are the clinical symptoms
skin penetration: L3 cause severe local itching (ground itch) at penetration site. erythematous papular rash develops with heavy larval invasion. pulmonary phase: Loeffler’s syndrome - in heavy infections = respiratory symptoms (cough and wheezing) intestinal phase: ○ Asymptomatic infection is common in mild cases. ○ Adult worms feed on blood using anticoagulants , causing persistent bleeding even after moving feeding sites. ○ Stool may become black (melaena) due to continued blood loss. ○ Chronic infections lead to iron deficiency anemia , with symptoms like: ■ Exertional dyspnea ■ Fatigue ■ Palpitations ■ Dizziness
28
in Hookworms, severe anemia can result in
cardiac failure hypoalbuminemia
29
what is the clinical symptoms of pulmonary phase in hookworm
Loeffler's syndrome - respiratory symptoms like cough and wheezing.
30
what is the clinical symptoms of intestinal phase when there is a continued blood loss
stool may become black (melaena)
31
hookworm diseases
Ancylostoma duodenale: Ancylostomiasis Necator americanus: Necatoriasis - nutrition competition may lead to microcytic hypochromic anemia (IDA), weakness, hypoproteinemia. - severe cases can cause massive blood loss and mortality.
32
what happens if there is a repeated infection in hookkworms
intense allergic itching (ground itch)
33
in hookworms, what happens if there is larval migration to the lungs
soar throat bloody sputum wheezing headache mild pneumonia
34
in hookworms, what are the intestinal symptoms that depends on worm burdern
○ Chronic infection (<500 eggs/g feces): Mild gastrointestinal discomfort, slight anemia, weight loss, melaena, weakness ○ Acute infection (>5000 eggs/g feces): Diarrhea, anorexia, edema, pain, enteritis, epigastric discomfort
35
what is the function of the hookworm's teeth/ cutting plates?
They use these to attach to the wall of the small intestine. Once attached, they suck blood and absorb nutrients from the host’s body to survive and grow.
36
Hookworm treatment and prevention and control
TREATMENT Medications to Kill Worms (Anthelmintics): - Albendazole (400 mg, single dose) or - Mebendazole (100 mg twice daily for 3 days OR 500 mg single dose) - Pyrantel pamoate (11 mg/kg daily for 3 days) – safe for pregnancy Treating Anemia (Low Blood Count): - Iron supplements to restore lost iron - Severe cases may require blood transfusion - Important: If hemoglobin (blood levels) is very low, do not give anthelminthic drugs (deworming medicine) first—correct anemia first to avoid complications. PREVENTION AND CONTROL - proper sanitation practices, especially appropriate fecal disposal - prompt and thorough treatment of infected persons, and personal protection such as boots.
37
when should we deworm the pets
every 2 months
38
briefly explain the animal nematodes that can infect humans
transes condition: 1. cutaneous larva migrans (CLM) 2. visceral larva migrans (VLM) *humans are accidental hosts for these kind of diseases
39
"Creeping Eruption" - etiologic agent - skin manifestation - other organ systems affected
Cutaneous Larval Migrans [It’s called “Creeping Eruption” because the rash looks like it's crawling under the skin] Etiologic Agent: Filariform of Ancylostoma family (Ancylostoma braziliense , most common). Skin Manifestations: Progression from July 4th - 11th. Other Organ Systems Affected: ○ Respiratory System: Larval migration to the lungs may affect the respiratory system = bronchitis or pneumonitis. ○ Gastrointestinal System: Maturation and migration of worm to the intestine = abdominal pain, steatorrhea, or sometimes diarrhea.
39
hookworms and its no./ pairs of teeth
Necator americanus: 0 - has cutting plates Ancylostoma braziliense: 1 pair Ancylostoma caninum: 3 pairs Ancylostoma duodenale: 2 pairs
40
smallest intestinal nematode of man
Strongyloides stercoralis
40
Strongyloides stercoralis - common name - scientific name - habitat - diseases caused - definitive host: - intermediate host: - infective stage: - diagnostic stage:
Common Name: Threadworm Scientific Name : Strongyloides stercoralis Habitat: Small Intestine - female adult worms are embedded in the mucosa of the S.I of humans. Disease Caused: ○ Strongyloidiasis ○ Cochin-china diarrhea ○ Lobar Pneumonia (if migrated to the lungs) Definitive Host : Human Intermediate Host: None Infective Stage: Filariform Larva Diagnostic Stage: Rhabditiform larvae
41
distribution of Strongyloides stercoralis
Mainly found in the tropics; also occurs in temperate regions. *S. fullerborni: infects African monkeys, pygmies in Africa, and humans in Papua New Guinea.
42
morphology of Strongyloides stercoralis
Smallest intestinal nematode in humans. Thin, about 2.5 mm long. Parthenogenetic (reproduces without males). Ovoviviparous (eggs hatch inside the female) (transes)
43
reproduction of of Strongyloides stercoralis
only female worms exist in human infections male worms are absent
44
it lay eggs without the need of fertilization by a male. So this is a highly adaptive mechanism enabling auto infection and persistent infection for decades.
Parthenogenesis: - it allows a single female to sustain infection cycles making eradication more difficult without the treatment
45
what is thelife cycle of Strongyloides sterocoralis
Unlike hookworms, where eggs are primarily excreted in feces, threadworms primarily pass rhabditiform larvae (L1) in feces. three possible life cycle routes: - direct (similar to hookworms) - indirect (free-living stage in the enviro) - autoinfection (continous reinfection w/o leaving the host) *internal autoinfection *external autoinfection