Mycoses I Flashcards

1
Q

What are fungi, and how do they differ from plants?

A

they are eukaryotic chemoheterotrophic microorganisms that do not contain chlorophyll

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2
Q

What are chemoheterotrophs and saprobes, and what role do fungi play in ecosystems?

A

chemoheterotrophs absorb organic matter from the environment and most fungi are saprobes that feed on dead or decaying matter

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3
Q

what are Mycoses?

A

diseases caused by pathogenic fungi that feed on living plants or animals

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4
Q

Many serious (systemic) fungal infections are caused by

A

opportunistic pathogens, which mainly affect people with weakened immune systems

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5
Q

what are the two forms of fungi used to make antibiotics?

A

Molds and yeasts

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6
Q

what is dimorphism in fungi

A

fungi can change their form depending on environmental conditions — specifically temperature
- molds at cooler temperatures
- yeasts at warmer temperatures

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7
Q

what are examples of dimorphic fungi?

A

Histoplasma capsulatum Cryptococcus neoformans and Candida albicans

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8
Q

what is the cell structure of fungi?

A
  • cell walls contain chitin, glucans and mannans
  • cell membrane contains sterols (ergosterols)
  • one nuclei surrounded by a nuclear membrane
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9
Q

what is the structure of molds?

A

Molds are made of thread-like filaments called hyphae, which grow, branch out and secrete enzymes to break down complex materials into nutrients they can absorb — a process called extracellular digestion

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10
Q

what happens when hyphae clump together?

A

they form a mycelium, which acts like a feeding network and also contain reproductive structures

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11
Q

what are septate hyphae?

A

hyphae that have cross-walls called septa that divide the hypha into compartments but are incomplete, allowing nutrients, organelles, and even nuclei to flow freely through them

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12
Q

what are non-septate hyphae (Coenocytic)?

A

species that do not have septa, so the entire hypha is one continuous cell but still contain multiple nuclei, floating freely throughout the structure

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13
Q

what are the two main phases of the fungal life cycle?

A
  1. Asexual Phase: vegetative growth and reproduction without fusion of gametes (most common)
  2. Sexual Phase: Occurs less often and involves the fusion of nuclei from two different fungal cells
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14
Q

what is sporulation?

A

main way fungi reproduce and spread by forming spores which land in a suitable environment, germinate and form hyphae

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15
Q

what are the types of asexual spores (conidia)?

A

In Molds:
- Sporangiospores
- Conidiospores
- Arthrospores
- Chlamydospores
In Yeasts:
- Blastospore

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16
Q

what are sporangiospores?

A

spores made by fungal hypha inside a structure called a sporangium that releases the spores when it bursts open

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17
Q

what are conidiospores?

A

exposed, dust-like fungal spores that bud off from hyphae without being enclosed in a sac and spread easily in air

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18
Q

what are arthrospores?

A

spores formed by fragmentation of fungal hyphae by the hypha breaking apart into individual cells

19
Q

what are chlamydospores?

A

Thick-walled, round or oval spores formed inside hyphae or at the tips of hyphae that are not meant for spreading, but for surviving

20
Q

how do yeast reproduce (asexual)?

A

through a process called budding where a small part of the yeast cell swells and forms a bud that grows and becomes a new cell called a blastospore which eventually breaks off and lives on its own

21
Q

what are pseudohyphae?

A

elongated chain of yeast cells that don’t completely separate from the parent cell during budding and that have less rigid walls

22
Q

what are the main fungi that reproduce sexually?

A

Zygomycota
Ascomycota
Basidiomycota

23
Q

Blastomycota

A

yeast that reproduces asexually through budding and form blastoconidia (blastospore)

24
Q

how does sexual reproduction occur in Zygomycota?

A

fungal body called the mycelium contain a “+” mating type and a “–” mating type that fuse together to form a zygospore which then has its nuclei separate in a sporangium to form sporangiospores that are later released

25
what is a zygospore?
heterokaryotic reproductive structure, that contains many different nuclei (with genetically different DNA) from both parent cells and multiple chromosomes
26
how does sexual reproduction occur in Ascomycota?
Two haploid hyphae (+ and −) grow and fuse together inside the mycelium to create heterokaryotic hyphae that produce multiple ascus inside an ascocarp (sac) - inside each ascus two nuclei fuse to form a diploid zygote which undergoes meiosis to produce ascospores that are released upon maturation
27
how does sexual reproduction occur in Basidiomycota?
inside small, club-shaped cells called basidia found on the gills underneath the basidiocarp cap the nuclei fuse to form a zygote (diploid) that undergoes meiosis and mitosis to produce haploid basidiospores that are released into the air
28
what is a basidiocarp?
a mushroom - basidiospores land and new hyphae (+ and -) fuse together to form a basidiocarp
29
what are the sources of infection for fungal diseases?
1. Geophilic - soil 2. Zoophilic - animals 3. Anthropophilic - human to human
30
how do fungi cause infections?
adhering to surfaces, invading tissues using hyphae and enzymes, and resistance to immune system attacks
31
how does humoral immunity protect against fungal infections?
Antibodies can tag fungi for destruction (opsonization) and they also activate complement
32
how does cell-mediated immunity protect against fungal infections?
T-cells, especially Th17 cells, are the main line of defense and critical for fighting fungal infections
33
how do phagocytic interactions protect against fungal infections?
Neutrophils and macrophages are white blood cells that engulf and kill fungal hyphae
34
how does inflammation cause fungal infections?
when fungi stay in the body too long, the immune system keeps fighting them which can cause tissue damage and inflammation, contributing to symptoms and disease
35
how are fungi are diagnosed?
1. Histological Detection 2. Macroscopic Characteristics 3. Microscopic Characteristics 4. Biochemical Testing
36
Histological Detection (Microscopic Staining)
a stain called Periodic Acid-Schiff (PAS) is used on tissue samples and highlights fungal elements in pink/magenta under a microscope, making them easier to see
37
Macroscopic Characteristics (How Cultures Look)
Fungi are grown on a medium like Sabouraud's Dextrose Agar (SDA) and colony morphology and color is observed
38
Microscopic Characteristics (Spore Type & Structure)
used to identify specific species through LPCB stain (Lactophenol Cotton Blue) to observe: - Types of spores - Shape, size, and how spores are arranged or attached to hyphae
39
Biochemical Testing
Especially for yeasts, tests are done to check: - Sugar fermentation - Sugar assimilation - Gas production
40
what are the main anti-fungal therapies?
1. Polyenes 2. Azole drugs 3. Allyamines 4. Cell Division Inhibitors
41
MOA for Polyenes
Bind to ergosterol in the fungal cell membrane, create pores → cell contents leak out → fungal cell dies. - examples: Nystatin, Amphotericin B
42
MOA for Azole Drugs
Inhibit lanosterol demethylase, an enzyme crucial for converting lanosterol to ergosterol - examples: Imidazoles, Ketoconazole
43
MOA for Allylamines
Inhibit the enzyme squalene epoxidase, stopping squalene from turning into ergosterol - examples: Terbinafine
44
MOA for Cell Division Inhibitors
- Griseofulvin: blocks mitosis by interfering with the mitotic spindle which prevents proper separation of chromosomes during cell division - Flucytosine: gets taken up by fungi and converted to a compound that inhibits DNA and protein synthesis