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Microbiology > MYCOLOGY TESTS AND AGARS > Flashcards

MYCOLOGY TESTS AND AGARS Flashcards

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Biology 101 flashcards
1
Q

purpose of this test is to dissolve the non-fungal materials, digest debris, and make fungal structures more visible

A

10-20% koh

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2
Q

KOH conc for hair and skin

A

10%

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3
Q

KOH conc for nails

A

20%

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4
Q

most widely used staining method for fungi

A

lactophenol cotton blue

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5
Q

lactophenol cotton blue components and its use

lactic acid:
phenol:
cotton blue:

A

lactic acid: preservative
phenol killing agent:
cotton blue: stain

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6
Q

negative stain used in mycology

A

india ink/nigrosin

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7
Q

why do we use india ink? what do we detect?

A

to demonstrate the capsule of cryptococcus neoformans

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8
Q

fluorochrome dye; best for direct microscopy

A

calcofluor white stain

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9
Q

purpose of calcofluor white

A

demonstrate chitin

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10
Q

t or f. all fungi are gram positive

A

true

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11
Q

if we do gram staining of fungi, what do we add? and what is its name after?

A

ammonium oxalate to crystal violet;

huckers modification

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12
Q

used to detect fungi in tissues; demonstrate details of fungal elements

A

periodic acid schiff

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13
Q

PAS color theme (spx, background)

A

spx: pink-red color bright magenta
bg: green

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14
Q

used to detect Nocardia and B. dermatidis

A

kinyoun’s

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15
Q

stain used to demonstrate the yeast of H. capsulatum

A

giemsa or wright’s

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16
Q

demonstrates melanin; best permanent mount

A

gomori methenamine silver

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17
Q

demonstrates malassezia furfur

A

acridine orange

18
Q

detection of hyphal pigmentation; determine if hyaline or dematiaceous (give pigment type)

A

fontana masson

hyaline - light
dematiaceous - dark

19
Q

to demonstrate cryptococcus neoformans

A

mayer’s mucicarmine

20
Q

enumerate the things needed for fungal culture

temp:
growing time:
what are added to cultures:
identified based on:

A 
25-30 C
30 days
what are added:
antibacterial agents
colony morphology and pigmentation and microscope
21
Q

added to fungal cultures to prevent the overgrowth of bacteria`

A

chloramphenicol

22
Q

added to fungal cultures to prevent growth of contaminants like saprophytic fungi

A

cycloheximide

23
Q

general purpose fungi media

A

sabouraud’s dextrose agar

24
Q

screening media for dermatophytes

indicator:

A

dermatophyte test medium

phenol red

25
Q

media for recovery of dermatophytes

A

mycosel/mycobiotic agar

26
Q

used to stimulate chlamydospore formation of Candida albicans

A

cornmeal agar

27
Q

isolation media for aspergillus

A

czapek agar

28
Q

media used for cryptococcus neoformans

A

niger seed agar/staib’s

29
Q

media used to demonstrate pigment production of T. rubrum

rubrum pigment:
mentag pigment:

A

potato dextrose agar

rubrum: red
mentag: none

30
Q

medium for id of M. audouinii

A

rice medium

31
Q

biochemical test media; differentiates mentag from rubrum

A

urea agar

mentag is urease +

32
Q

general media used for yeast spp

A

BHI

33
Q

medium suited for nocardia

A

casein medium

34
Q

media used for malassezia furfur

A

malt extract agar

35
Q

media used to recover saprophytes and pathogenic fungi

A

potato flake agar

36
Q

special test to differentiate mentag and rubrum in hair strands

A

hair perforation/baiting test

37
Q

special test for rapid id of neoformans

A

L-DOPA ferric citrate test

38
Q

presumptive test for candida albicans

A

germ tube

39
Q

special to differentiate c. albicans from c. dubliniensis

A

temperature studies (42 C)

40
Q

temperature studies results:

A

positive - albicans

negative - dubliniensis, neoformans

Microbiology (18 decks)

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