MYCOLOGY TESTS AND AGARS Flashcards
purpose of this test is to dissolve the non-fungal materials, digest debris, and make fungal structures more visible
10-20% koh
KOH conc for hair and skin
10%
KOH conc for nails
20%
most widely used staining method for fungi
lactophenol cotton blue
lactophenol cotton blue components and its use
lactic acid:
phenol:
cotton blue:
lactic acid: preservative
phenol killing agent:
cotton blue: stain
negative stain used in mycology
india ink/nigrosin
why do we use india ink? what do we detect?
to demonstrate the capsule of cryptococcus neoformans
fluorochrome dye; best for direct microscopy
calcofluor white stain
purpose of calcofluor white
demonstrate chitin
t or f. all fungi are gram positive
true
if we do gram staining of fungi, what do we add? and what is its name after?
ammonium oxalate to crystal violet;
huckers modification
used to detect fungi in tissues; demonstrate details of fungal elements
periodic acid schiff
PAS color theme (spx, background)
spx: pink-red color bright magenta
bg: green
used to detect Nocardia and B. dermatidis
kinyoun’s
stain used to demonstrate the yeast of H. capsulatum
giemsa or wright’s
demonstrates melanin; best permanent mount
gomori methenamine silver
demonstrates malassezia furfur
acridine orange
detection of hyphal pigmentation; determine if hyaline or dematiaceous (give pigment type)
fontana masson
hyaline - light
dematiaceous - dark
to demonstrate cryptococcus neoformans
mayer’s mucicarmine
enumerate the things needed for fungal culture
temp:
growing time:
what are added to cultures:
identified based on:
25-30 C 30 days what are added: antibacterial agents colony morphology and pigmentation and microscope
added to fungal cultures to prevent the overgrowth of bacteria`
chloramphenicol
added to fungal cultures to prevent growth of contaminants like saprophytic fungi
cycloheximide
general purpose fungi media
sabouraud’s dextrose agar
screening media for dermatophytes
indicator:
dermatophyte test medium
phenol red
