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Microbiology > MYCOLOGY SPECIMENS AND TESTS > Flashcards

MYCOLOGY SPECIMENS AND TESTS Flashcards

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Biology 101 flashcards
1
Q

when should we collect viral spx?

A

early/acute phase (within 72 hours)

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2
Q

viral swab material requirement

A

dacron fibers or nylon

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3
Q

mortal sin in swab

A

calcium alginate, cotton, or wooden

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4
Q

spx processing:

urine

A

neutralize w 7.5 calcium bicarbonate

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5
Q

spx processing:
blood
volume:
ac:

A

3-5ml

heparin/citrate

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6
Q

ac to be used for pcr methods

A

edta

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7
Q

ac that inhibits polymerase

A

heparin

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8
Q

viral transport media ph and indicator

A

7.2 phenol red

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9
Q

antibiotics used in viral transport media

A

streptomycin and penicillin

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10
Q

3 components of viral transport media

A

isotonic soln, protein, antibiotics

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11
Q

virus processing temp and time requirement

A

4C, up to 5 days

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12
Q

delay of 6 days or longer temp req

A

-20 or -70

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13
Q

respiratory spx and virus (3)

A

throat swab - influenza
nasal washings - parainfluenza
nasopharyngeal aspirate - RSV

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14
Q

gastroenteritis virus (2)

A

rectal swab - norwalk agent

stool - rotavirus

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15
Q

skin lesion virus (2)

A

skin scrapings - VZV

vesicular fluid - HSV

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16
Q

congenital infection virus

A

urine - CMV

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17
Q

meningitis virus

A

blood and csf - togavirus

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18
Q

virus that can be seen using light microscopy

A

pox virus

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19
Q

inclusion bodies:

ca of negri bodies

A

rabies virus

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20
Q

inclusion bodies:

ca of torres councilman bodies

A

yellow fever virus

21
Q

inclusion bodies:

ca of bollinger bodies

A

fowl pox virus

22
Q

inclusion bodies:

ca of guarneri paschen bodies

A

variola and varicella

23
Q

microscope used to detect the morphology of virus

A

electron microscopy

24
Q

indication of HPV infection

A

koilocytes

25
Q

smear that detects VZV or HSV inclusions

A

tzanck smear

26
Q

gold standard for virus detection

A

viral culture

27
Q

type of cell culture:

uses normal, mature adult cells

A

primary cell culture

28
Q

type of cell used in primary cell culture

A

rhesus monkey/rabbit kidney cells

29
Q

type of cell culture:

uses embryonic or fetal cells

A

diploid/semi continuous cell lines

30
Q

primary cell culture passage times

A

once or twice

31
Q

diploid cell lines passage times

A

50x

32
Q

type of cell used in diploid cell lines

A

fibroblasts from lung/foreskin

33
Q

diploid cell to isolate CMV

A

human embryonic fibroblast

34
Q

diploid cell to isolate VZV and HSV

A

human fetal diploid

35
Q

type of cell culture:

uses malignant or cancer cells

A

heteroploid/continuous cell line

36
Q

heteroploid passage times

A

unlimited

37
Q

heteroploid cell that uses cervical cancer cells

A

HeLa cells

38
Q

heteroploid cell that uses laryngeal cancer cells

A

Hep2 cells

39
Q

indication that there is a viral growth in cell cultur

A

cytopathic effect

40
Q

cytopathic effect examples

A 
pyknosis
fused cells
vacuoles in cytoplasm
swelling and clumping
proliferation of nuclear membrane
41
Q

CPEs:

condensation/shrinking of nucleus

A

pyknosis

42
Q

virus that can cause pyknosis

PYK PIC

A

picornavirus

43
Q

virus that can cause cell fusion

A

paramyxovirus

44
Q

virus that can cause vacuolation

A

polyomavirus and papillomavirus

45
Q

virus that can cause swelling and clumping

A

adenovirus

46
Q

virus that can cause nuclear membrane proliferation

A

alpha herpes virus

47
Q

this happens when there is no or little CPEs

A

hemadsorption

48
Q

virus that can cause hemadsorption

A

influenza, parainfluenza, mumps

Microbiology (18 decks)

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