G+ STAPHYLOCOCCI Flashcards

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1
Q

general characteristics of Staph. spp.

A

gram + cocci in cluster
catalase +
normal inhabitants of skin/mucus membrane

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2
Q

golden yellow pigment of S. aureus

A

lipochrome

due to carotenoid

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3
Q

found in cell wall of S. aureus that neutralizes IgG

A

protein A

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4
Q

virulence marker of S. aureus

A

coagulase

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5
Q

enzyme of S. aureus that digests clot

A

staphylokinase

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6
Q

enterotoxins associated with staph food poisoning

A

enterotoxin A, B, D

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7
Q

enterotoxins associated with toxic shock syndrome

A

enterotoxin B, C

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8
Q

enterotoxins associated with staphylococcal pseudomembranous enterocolitis

A

enterotoxin B

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9
Q

causes menstruating-associated TSS

A

toxic shock syndrome toxin 1

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10
Q

causes ritter disease/bullous impetigo

A

exfoliative/epidermolytic toxin

exfoliative toxin A, B

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11
Q

cytolytic toxins:
lyses rbcs
causes severe tissue damage

A

α-hemolysin

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12
Q

cytolytic toxins:
acts on the sphingomyelin
aka hot-cold lysin

A

β-hemolysin

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13
Q

cytolytic toxins:

associated with panton valentine leukocidin

A

γ-hemolysin

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14
Q

cytolytic toxins:

less toxic

A

δ-hemolysin

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15
Q

exotoxin that is lethal to pmn cells

associated with community acquired MRSA

A

panton valentine leukocidin

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16
Q

coagulase -

most common cause of nosocomial uti

A

S. epidermidis

17
Q

sensitive to novobiocin

>16 mm

A

S. epidermidis

18
Q

resistant to novobiocin

<16 mm

A

S. saprophyticus

19
Q

coagulase -

causes acute uti in young women

A

S. saprophyticus

20
Q

coagulase - but with clumping factor
can mimic S. aureus infections
contains mecA gene

A

S. lugdunensis

21
Q

primary plate for Staph spp.

A

BAP (SBA)

22
Q

differential

A

MSA

S. aureus can ferment mannitol

23
Q

special media for isolation of MRSA

A

chromagar staph aureus

24
Q

test used to differentiate S. aureus from other Staph spp.

A

coagulase test

25
Q

coagulase: slide detects?

A

clumping factor/cell bound coagulase

26
Q

coagulase: tube detects?

A

free coagulase/unbound coagulase

27
Q

S. aureus on PYR test

A

negative (no color change)

28
Q

differentiates Micrococci from Staphylococci

give also the results

A

modified oxidase test
+ - Micrococci
- - Staphylococci

29
Q

bacteria with _____ will produce a dark-blue product in modified oxidase

A

cytochrome C

30
Q

S. aureus on DNAse test

A

positive (hydrolysis, clearing)