mycology lab quiz

Question 1

tinea unguium

Answer 1

infection of the nail

Question 2

tinae corporis

Answer 2

body

Question 3

tinae pedis

Answer 3

foot

Question 4

tinae barbay

Answer 4

beard

Question 5

tinae cruris

Answer 5

groin

Question 6

what does the word tinae mean?

Answer 6

latin word for ringworm

Question 7

name the short lateral structure that will develop when certain yeast are incubated at 37 degrees in a rich medium

Answer 7

germ tube

Question 8

name one yeast that is germ tube positive

Answer 8

candida albicans

Question 9

at what temperature should chlamydospore agar be incubated?

Answer 9

25 degrees

room temp.

Question 10

where should the technologist look for spores on a chlamydospore agar plate?

Answer 10

edge of the coverslip

Question 11

what color and shape are the spores on a chlamydospore plate?

Answer 11

blue and round

Question 12

what is the principle of the india ink preparation

Answer 12

drop of india ink and drop of organism
then look on high power will see halo around the budding yeast because particles of the india ink bounce off the unstained capsule

Question 13

what organism is india ink especially used for?

Answer 13

cryptococcus neoformans

Question 14

what type of specimen is most frequently used for india ink ?

Answer 14

CSF

Question 15

what reagent should be used for the examination of the reproductive structures of molds that are already growing in a culture?

Answer 15

lactophenol cotton blue (LPCB)

Question 16

what technique can be used to “save” wet preps for future use?

Answer 16

clear fingernail polish all around coverslip

Question 17

name several media used for the primary isolation of fungi

Answer 17

sabsC
SDA
sabourauds
BHI

Question 18

why would you incubate a culture for fungi at 25 degrees and 37 degrees

Answer 18

to demonstrate both phases (dimorphic/diphasic)

mold is at 25 and yeast is at 37

Question 19

what term is used for fungi that grow differently at these temperatures

Answer 19

dimorphic

Question 20

why do mycologists use KOH

Answer 20

to clear keratin so fugal elements will stand out

Question 21

what specific protein does KOH affect?

Answer 21

keratin

Question 22

name at least two fungal elements that could be seen in a KOH prep

Answer 22

hyphae
pseudohyphae
arthrospores
blastospores
budding yeast

Question 23

what is the difference between ectothrix and endothrix hair invasion

Answer 23

ectothrix is outside the hair shaft

endothrix is inside the hair shaft

Question 24

name one genus of fungus which causes ringworm

Answer 24

microsproum
epidermophyton
trichophyton

Question 25

name one genus of fungus which exhibits dimorphism

Answer 25

blastomyces
coccidiodes
paracoccidiodes
histoplasma sporothrix

Question 26

name of genus of fungus which is a frequent contaminant

Answer 26

aspergillus

penicillium

Question 27

what is a slide culture?

Answer 27

a fungus on slab of agar with cover slip allowing to grow on cover slip then consequently viewing perfect morphology
best way to observe fungi in natural state