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Microbiology:Bacteriology > Mycobacteria > Flashcards

Mycobacteria Flashcards

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Biology 101 flashcards
1
Q

cell wall content of Mycobacteria

A

N-glycol muramic acid

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2
Q

appearance of Mycobacteria when incomplete staining is done

A

Beaded appearance

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3
Q

pH of Mycobacteria

A

6.5-6.8

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4
Q

Mycobacterium tuberculosis complex

A 
Mycobacterium tuberculosis
Mycobacterium bovis
Mycobacterium africanum
Mycobacterium canetti
Mycobacterium microti
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5
Q

colonial morphology of M. tuberculosis

A

cauliflower colonies

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6
Q

used for BCG vaccine

A

M. bovis

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7
Q

smooth strain of M. tuberculosis

A

M. carnetti

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8
Q

chronic granulomatous infection

A

tuberculosis

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9
Q

airborne nuclei in tuberculosis

A

1-5 um

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10
Q

characteristic of tubercle

A

cheesy like consistency

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11
Q

calcified tubercle that can be identified in chest x-rays

A

Ghon complexes

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12
Q

tuberculosis spondylitis

A

Pott’s disease

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13
Q

extrapulmonary tuberculosis

A

miliary tuberculosis

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14
Q

classification of Mycobacteria

A

Runyon’s classification

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15
Q

photochromagens

A 
"simi is a marine that lived in asia then kansas"
M. kansasii
M. asiaticum
M. marinum
M. simiae
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16
Q

scotochromagens

A 
"gordon tasted a flavored scroful by zul in a xenopi"
M. szulgai
M. gordonae
M. scrofulaceum
M. flavescens
M. xenopi
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17
Q

non-photochromagens

A 
"bloody birds in my terrace cause ulcers in my GIT"
M. avium complex
M. ulcerans
M. terrae complex
M. gastri
M. haemophilum
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18
Q

rapid growers

A 
M. fortuitum
M. chelonae
M. smegmatis
M. phlei
M. abcessus
M. mucogenicum
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19
Q

characteristics of photochromagens

A

cream/buff yellow orange under the light

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20
Q

characteristics of scotochromagens

A

yellow orange under the light and dark

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21
Q

most common non-M. tuberculosis in AIDS patients

A

M. avium complex

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22
Q

causative agent of Johne’s/Crohn’s disease

A

M. avium subspp. paratuberculosis

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23
Q

colonial morphology of M. kansasii

A

rough colonies with dark centers and waxy edges

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24
Q

causative agent of swimming pool granuloma with red and blue nodule

A

M. marinum

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25
Q

causative agent of Buruli ulcer

A

M. ulcerans

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26
Q

colonial morphology of M. xenopi

A

bird’s nest appearance

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27
Q

Mycobacterium terrae complex

A

M. terrae
M. triviale
M. nonchromogenicum

28
Q

most common rapidly growing mycobacteria

A

M. fortuitum

29
Q

associated with outbreaks from DPT polio vaccines, histamine and lidocaine injection

A

M. chelonae

30
Q

M. smegmatis group

A

M. smegmatis sensu strict
M. goodie
M. wolinskyi

31
Q

best specimen for Mycobacterium tuberculosis

A

sputum

32
Q

criteria for sputum

A

<10 epithelial cells

>25 pus cells

33
Q

best specimen for non-Mycobacterium tuberculosis

A

bronchoscopy

34
Q

specimen for M. avium in patient with AIDS

A

feces

35
Q

number of organisms to obtain a positive result

A

5,000 to 10,000 organisms/ml

36
Q

sensitive, reliable and specific stain for M. tuberculosis

A

Auramine-rhodamine

37
Q

positive stain for auramine-rhodamine

A

bright yellow, orange against a dark background

38
Q

number of field counted before reporting as negative

A

300 OIO fields

39
Q

egg-based medium

A

Lowenstein-Jensen
American Thoracic Society
Petragnani
Wallenstein

40
Q

serum-based TRANSPARENT media

A

Middlebrook 7H10
Middlebrook 7H11
Mitchison’s medium

41
Q

Liquid broth

A

Middlebrook 7H12

Middlebrook 7H13

42
Q

most common medium for M. tuberculosis

A

LJ medium

43
Q

medium for CSF and bone marrow samples

A

American Thoracic Society

44
Q

medium for heavy contamination

A

Petragnani

45
Q

medium for the growth of M. avium

A

Wallenstein

46
Q

most common decontaminant and digestion agent

A

2-4% sodium hydroxide

47
Q

decontamination agent with 5% concentration mostly used for sputum

A

Oxalic acid

48
Q

decontaminant and digestive agent for sputum with large numbers of bacteria

A

Zephiran trisodium phosphate

49
Q

decontaminating agent in zephiran trisodium phosphate

A

zephiran

50
Q

digestive agent in zephiran trisodium phosphate

A

trisodum

51
Q

buffer agent in zephiran trisodium phosphate

A

phosphate

52
Q

decontaminant and digestive agent for sputum for prolonged shelf life (8 days)

A

1% pyridium chloride

53
Q

most common biochemical test for Mycobacterium tuberculosis (positive result)

A

Niacin (nicotinic acid) test

54
Q

indicator system in nitrate reduction test for Mycobacterium tuberculosis

A

zinc

55
Q

test to differentiate M. tuberculosis and M. bovis

A

thiophene-2-carboxylic acid hydrazide (T2H)

56
Q

principle of thiophene-2-carboxylic acid hydrazide (T2H)

A

inhibition

57
Q

positive microorganism in thiophene-2-carboxylic acid hydrazide test

A

M. bovis

58
Q

Mycobacteria that invades the peripheral nerve

A

Mycobacterium leprae

59
Q

morphology of M. leprae

A

cigar pocket/pocket fence appearance

60
Q

culture media for M. leprae

A

Armadillo

61
Q

definitive test for M. leprae

A

foot pads of mice

62
Q

disease caused by M. leprae

A

Hansen’s disease/ leprosy

63
Q

characteristics of tuberculoid leprosy

A 
localized
benign
non-progressive
loss of sensation
neural
64
Q

characteristics of lepromatous leprosy

A

malignant
progressive
loss of facial features

65
Q

test for to differentiate the two types of leprosy

A

Lepromin

66
Q

positive for lepromin

A

tuberculoid/neural leprosy

Microbiology:Bacteriology (10 decks)

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