Gram Positive Cocci Flashcards
Arrangement of Staphylococcus
clusters
best sample for Staphylococcus
aspirates
hemolytic pattern of Staphylococcus
beta-hemolytic
culture media for Staphylococcus
BAP
MSA
PEA
CNA
units of bacitracin
0.04u
resistant to bacitracin
Staphylococcus
units of furazolidone
100ug
resistant to furazolidone
Micrococcus
positive to modified oxidase test
Micrococcus
resistant to lysostaphin test
Micrococcus
positive for furoxone-tween 80 oil red o
Micrococcus
positive to glycerol with erythromycin
Staphylococcus
positive OF test (Fermentation)
Staphylococcus
yellow colonies in MSA
Staphylococcus aureus
components of MSA
1% mannitol
7.5% NaCl
phenol red
other name of scalded skin syndrome
Ritter’s syndrome
cause of toxic shock syndrome
enterotoxin F
best single criterion for pathogenicity in Staphylococcus
coagulase test
plasma used for coagulase test
rabbit plasma in EDTA
heat stable toxin
enterotoxin/neurotoxin
causes vomitting, heat stable, targets T cells
enterotoxin causing foodpoisoning
enterotoxin A,B,D
enterotoxin causing pseudomembranous enterocolitis
enterotoxin B
toxin causing Toxic shock syndrome
enterotoxin F
leukocidin
Panton-Valentine
action of leukocidin
targets WBCs
causes delta hemolysis
antiphagocytic component of cell wall of Staphylococcus
protein A
biochemical tests for Staphylococcus aureus
TELLURITE GLYCINE: black
VP: pos
DNASE: pos (methyl green dye)
PYR: neg
mode of attachment of S. epidermidis
poly-Y-DL-glutamic acid
novobiocin resistant Staphylococcus
Staphylococcus saprophyticus
Staphylococcus associated to endocarditis
Staphylococcus ludenensis
confers S. lugdinensis resistance to oxaillin
Mec A gene
tests that will differentiate coagulase positive staphylococci
PYR
VP
gold standard for MRSA detection
PCR
nucleic acid probes
Smith and Brown
hemolytic pattern
Lancefield
extraction of C carbohydrate
Academic/Bergey’s
temperature
flesh eating bacteria
Streptococcus pyogenes
antiphagocytic component of S. pyogenes
M protein
Epithelial cell attachment for S. pyogenes
F protein
respiratory attachment for S. pyogenes
lipotechoic acid
causes rash in scarlet fever
Exotoxin B
Streptolysin O Characteristics
labile
subsurface
WBC, platelet, tissue cells
stab in BAP
Streptolysin S characteristics
stable
surface
WBC
Bacitracin susceptible Streptococcus
Streptococcus pyogenes
common cause of neonatal meningitis
S. agalactiae
CAMP test reaction of Streptococcus agalactiae
arrowhead hemolysis
5 Groups of viridans streptococci
S. mitis S. mutans S. salivarius S. bovis S. anginosus
most commonly isolated viridans streptococci
S. mutans
D antigen positive streptococci
Enterococci
Enterococcus faecalis
most common isolate
requires cyanocobalamin for growth
Enterococcus faecalis
cause of lobar pneumonia
Streptococcus pneumoniae
most common cause of bacterial meningitis in adults
Streptococcus pneumoniae
colonial morphology of young Streptococcus pneumoniae
dome shape
colonial morphology of old Streptococcus pneumoniae
coin with raised rim
donut colonies
nutritionally variant streptococci
Abiotrophia
Granulicatella
*they need B6, cystine, pyridoxal
pyridoxal producing bacteria
E. coli
Klebsiella
Enterobacter
Yeasts
Streptococci-like microorganisms
Aerococcus- vancomycin S Gemella-easily decolorized Lactococcus Leuconostoc-cross reacts with Lancefield D Pediococcus- grow at 45 degrees