MYCO Flashcards

1
Q

FUNGI
- Eukaryotes: ___, ___, ___
Prokaryotes: ___, ___, ___

A

FUNGI
- Eukaryotes: multicellular, nucleated, membrane-bound organelles
Prokaryotes: unicellular, anucleated, non-membrane-bound organelles

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2
Q

FUNGI
* “___” = ___ - macroscopic fungi
* ___ - ___ nuclei, ___

A

FUNGI
* “spongos” = MUSHROOM - macroscopic fungi
* THALLOPHYTES - true nuclei, heterotrophic

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3
Q

Cell Structure - Fungi
Cell Type:
Cell Wall:
Cell Membrane:
Spores:

A

Cell Structure - Fungi
Cell Type: Eukaryotic
Cell Wall: Glucan, chitin
Cell Membrane: Ergosterol
Spores: Reproductive

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4
Q

Two Phases of Fungi:
1. YEAST
* ___
* grows at:
* colony:

  1. MOLD
    * ___
    * grows at:
    * colony:
A

Two Phases of Fungi:
1. YEAST
* unicellular
* grows at 37degC body temp
* creamy, resembles bacterial colony

  1. MOLD
    * multicellular
    * grows at 20-25 degC room temp
    * cottony, mycelial mass
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5
Q

MOLDS
MYCELIUM
* ___
* ___

HYPHAE
* ___
* ___

A

MOLDS
MYCELIUM
* collection of hyphae
* intertwining structured

HYPHAE
* fundamental unit of fungi
* web, tubelike / filaments

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6
Q

Parts of Hyphae:
AERIAL (REPRODUCTIVE)
* ___
* produces ___ - ___

VEGETATIVE (THALLUS)
* ___
* ___

A

Parts of Hyphae:
AERIAL (REPRODUCTIVE)
* above the surface
* produces conidia / SPORES - reproduction of fungi

VEGETATIVE (THALLUS)
* extends downwards
* absorbs water & nutrients

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7
Q

Types of Hyphae:
SEPTATE HYPHAE
* ___ cross walls
* example/s:

SPARSELY SEPTATE HYPHAE / ___ HYPHAE
* ___
* ___ cross walls
* example/s:

A

Types of Hyphae:
SEPTATE HYPHAE
* frequent cross walls
* ALL except Zygomycetes

SPARSELY SEPTATE HYPHAE / COENOCYTIC HYPHAE
* aseptate
* few cross walls
* Zygomycetes

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8
Q

Spores Involved in Asexual Reproduction:
CONIDIA - ___
A. MACROCONIDIA
B. MICROCONIDIA

A

Spores Involved in Asexual Reproduction:
CONIDIA - singly or multiply in long chains or clusters
A. MACROCONIDIA - large multicellular
B. MICROCONIDIA - small unicellular

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9
Q

Spores Involved in Asexual Reproduction:
BLASTOCONIDIA
* daughter cells ___
* ___
* example

A

Spores Involved in Asexual Reproduction:
BLASTOCONIDIA
* daughter cells bud off the mother cell and is pinched off
* budding off yeast
* Candida

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10
Q

Spores Involved in Asexual Reproduction:
CHLAMYDOCONIDIA
* ___ formed from ___
* end/tip:
* side:
* within:
* Examples:

A

Spores Involved in Asexual Reproduction:
CHLAMYDOCONIDIA
* thick-walled resistant spores formed from rounding up and enlargement of terminal hyphal cells
* end/tip: TERMINAL
* side: SESSILE
* within: INTERCALARY
* P. brasiliensis, C. albicans, Microsporum

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11
Q

Spores Involved in Asexual Reproduction:
ARTHROCONIDIA
* ___
* __ spores
* Examples:

A

Spores Involved in Asexual Reproduction:
ARTHROCONIDIA
* simple fragmentation of the mycelium
* jointed, barrel-shaped spores
* Coccidioides, Geotrichum, Trichosporon

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12
Q

Spores Involved in Asexual Reproduction:
SPORANGIOSPORES
* ___ that are produced ___
* Example:

A

Spores Involved in Asexual Reproduction:
SPORANGIOSPORES
* sporangia or sacs that are produced terminally on sporangiophore or aseptate hyphae
* Zygomycetes

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13
Q

Spores Involved in Sexual Reproduction:
ASCOSPORES
* ___
* ___

A

Spores Involved in Sexual Reproduction:
ASCOSPORES
* Wine skin / bladder
* saclike ascus

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14
Q

Spores Involved in Sexual Reproduction:
BASIDIOSPORES
* ___
* ___
* ___

A

Spores Involved in Sexual Reproduction:
BASIDIOSPORES
* Base / pedestal
* club-shaped basidium
* clamp connections

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15
Q

Spores Involved in Sexual Reproduction:
ZYGIOSPORES
* ___
* fusion of:

A

Spores Involved in Sexual Reproduction:
ZYGIOSPORES
* Yoke / join
* fusion of two identical cells from the same hyphae

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16
Q

Spores Involved in Sexual Reproduction:
OOSPORES
* ___
* fusion of:

A

Spores Involved in Sexual Reproduction:
OOSPORES
* Egg
* fusion of cells from two separate, non-identical hyphae

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17
Q

MYCOSES
SUPERFICIAL MYCOSES
* ___ part ___ = ___
* Common Example:

Skin (2):
Hair (2):

A

MYCOSES
SUPERFICIAL MYCOSES
* outermost part of the skin = no immune response
* Common Example: DANDRUFF

Skin:
Malessezia furfur
Hortaea werneckii

Hair:
Piedraia hortae
Trichosporon beigielli

18
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
* Pale patches, dry scaly skin

A
  • Malessezia furfur
  • TINEA VERSICOLOR / PITYRIASIS VERSICOLOR
19
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
* Brown to black nonscaly

A
  • Hortaea werneckii
  • TINEA NIGRA
20
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
* Brown to black crusts / nodules on hair

A
  • Piedraia hortae
  • BLACK PIEDRA
21
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
* Light brown soft nodules

A
  • Trichosporon beigielli
  • Light brown soft nodules
22
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Spaghetti and meatballs

A
  • Malessezia furfur
  • TINEA VERSICOLOR / PITYRIASIS VERSICOLOR
23
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Wood’s lamp fluorescence (+)

A
  • Malessezia furfur
  • TINEA VERSICOLOR / PITYRIASIS VERSICOLOR
24
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Lipophilic

A
  • Malessezia furfur
  • TINEA VERSICOLOR / PITYRIASIS VERSICOLOR
25
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Greenish black colonies

A
  • Hortaea werneckii
  • TINEA NIGRA
26
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Asci containing ___

A
  • Piedraia hortae
  • BLACK PIEDRA
  • 8 ascopores
27
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Brown restricted colonies

A
  • Piedraia hortae
  • BLACK PIEDRA
28
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Arthroconidia

A
  • Trichosporon beigielli
  • WHITE PIEDRA
29
Q

MYCOSES
SUPERFICIAL MYCOSES

Identify organism and infection
Lab Diagnosis: Yeast-like colonies

A
  • Trichosporon beigielli
  • WHITE PIEDRA
30
Q

MYCOSES
CUTANEOUS MYCOSES - ___

A
  • elicit an immune response
31
Q

DERMATOPHYTES - cause ___

___ - red pigment on Cornmeal agar

A
  • Cutaneous mycoses
  • T. rubrum
32
Q

Summary of Body Parts Affected

Organism: Epidermophyton
Hair:
Skin:
Nails:

A

Summary of Body Parts Affected

Organism: Epidermophyton
Hair: ❌
Skin: ☑️
Nails: ☑️

33
Q

Summary of Body Parts Affected

Organism: Microsporum
Hair:
Skin:
Nails:

A

Summary of Body Parts Affected

Organism: Microsporum
Hair: ☑️
Skin: ☑️
Nails: ❌

34
Q

Summary of Body Parts Affected

Organism: Tricophyton
Hair:
Skin:
Nails:

A

Summary of Body Parts Affected

Organism: Tricophyton
Hair: ☑️
Skin: ☑️
Nails: ☑️

35
Q

Differential Tests on Trichophyton spp.

Organism: T. mentagrophytes
Urease:
Thiamine:
Hair baiting:

A

Differential Tests on Trichophyton spp.

Organism: T. mentagrophytes
Urease: (+) (2d)
Thiamine: (-)
Hair baiting: (+) (ectothrix)

36
Q

Differential Tests on Trichophyton spp.

Organism: T. rubrum
Urease:
Thiamine:
Hair baiting:

A

Differential Tests on Trichophyton spp.

Organism: T. rubrum
Urease: (-) (7d)
Thiamine: (-)
Hair baiting: (-)

37
Q

Differential Tests on Trichophyton spp.

Organism: T. tonsurans
Urease:
Thiamine:
Hair baiting:

A

Differential Tests on Trichophyton spp.

Organism: T. tonsurans
Urease: (+) (2d)
Thiamine: (+)
Hair baiting: (-) (endothrix)

38
Q

Differentiate ECTOTHRIX and ENDOTHRIX

A

ECTOTHRIX - outer part of the hair

ENDOTHRIX - inner part of the hair

39
Q

Differential Tests on Microsporum spp.

GROWTH IN RICE GRAINS
Poor Growth:
Good Growth:

A

Differential Tests on Microsporum spp.

GROWTH IN RICE GRAINS
Poor Growth: M. audouinii
Good Growth: M. canis, M. gypseum

40
Q

Differential Tests on Microsporum spp.

GROWTH IN RICE GRAINS Procedure:

A

Differential Tests on Microsporum spp.

GROWTH IN RICE GRAINS
1. Place colony of culture of fungi in rice
2. Incubate at room temp
3. Check for growth (good or poor)
Good growth = cotton candy

41
Q

Differential Tests on Microsporum spp.

WOOD’S LAMP FLUORESCENCE (364 nm)
(+) =
(-) =

A

Differential Tests on Microsporum spp.

WOOD’S LAMP FLUORESCENCE (364 nm)
(+) = M. audouinii, M. canis
(-) = N. gypseum

42
Q

A

A