BB

Question 1

Single blood donation to multiple patients:

Answer 1

• COMPONENT PREPARATION
• RBCs, platelets, fresh-frozen plasma, cryoprecipitate, plasma derivatives

Question 2

EQUIPMENT IN THE COMPONENT MANUFACTURING LABORATORY:
1. CENTRIFUGES
- spin a maximum of ___ at once
- ___; ___ can be changed based on ___

A. HARD SPIN
- depends on ___
B. SOFT SPIN
- not remove ___ out of ___

Answer 2

• 6-12 units of WB
• programmable speed (RPM), time settings, components
• length / duration & speed
• plasma, RBC

Question 3

AABB-RECOMMENDED CENTRIFUGE CONDITIONS
Components Prepared - Centrifuge Conditions

RBCs + Plasma:
RBCs + Platelet-Rich Plasma:
Harvesting Platelets / Cryoprecipitate from Plasma:

Answer 3

RBCs + Plasma: 5,000 x g, 5 minutes
RBCs + Platelet-Rich Plasma: 2,000 x g, 3 minutes
Harvesting Platelets / Cryoprecipitate from Plasma: 5,000 x g, 7 minutes

Question 4

EQUIPMENT IN COMPONENT MANUFACTURING LABORATORY:
2. PLASMA EXPRESSORS
- apply ___ to the ___
- blood components flow ___ by ___

Answer 4

• pressure, blood bag
• flow from one bag to another, integrated tubing system

Question 5

EQUIPMENT IN COMPONENT MANUFACTURING LABORATORY:
3. TUBING SEALERS
- tubing that connects blood bags:

Answer 5

PVC (POLYVINYL CHLORIDE) - resistant to damage

Question 6

EQUIPMENT IN COMPONENT MANUFACTURING LABORATORY:
4. STERILE CONNECTION DEVICES (SDCs)
- ___ connected via ___ w/o ___
- each piece of tubing is placed in a ___ and ___

Answer 6

• 2 separate blood bags, PVC tubing, breaching the integrity of either container
• channel, clamped

Question 7

EQUIPMENT IN THE COMPONENT MANUFACTURING LABORATORY:
5. STORAGE DEVICES
- carefully selected and validated; ___
- temp must be ___, ___ at least ___
- ___ if ___

Answer 7

• FDA-regulated storage temperatures
• continuously monitored, recorded, EVERY 4 HOURS
• alert, unacceptable temperature

Question 8

EQUIPMENT IN THE COMPONENT MANUFACTURING LABORATORY:
6. QUADRUPLE BAG
- ___ depending on the ___

MOTHER BAG - ___ (___)
SATELLITE BAGS (___) - ___ —> ___ will be transferred ___

Answer 8

• triple bags, component that will be produced
• primary component (whole blood)
• 2, expression, separate component, directly

Question 9

BLOOD COMPONENTS:

Answer 9

OXYGEN-CARRYING PRODUCTS

Question 10

THE WHOLE BLOOD AND ITS COMPONENTS:
WHOLE BLOOD
- ___
- ___ - ___

Answer 10

• mother / primary component
• unmodified - directly from the donor

Question 11

WHOLE BLOOD
Indications:

Answer 11

Indications:
Active bleeding
Hemorrhagic shock
Exchange Transfusion
Both oxygen-carrying capacity and volume expansion required

Question 12

WHOLE BLOOD
Shelf-life:
21 DAYS:
35 DAYS:
42 DAYS:

Answer 12

WHOLE BLOOD
Shelf-life:
21 DAYS: CPD, ACD, CP2D
35 DAYS: CPD-A1
42 DAYS: CPD-AS-1 (Adsol), AS/3 (Nutricel), AS-5 (Optisol)

Question 13

WHOLE BLOOD
Shelf-life:
___: CPD, ACD, CP2D
___: CPD-A1
___: CPD-AS-1 (Adsol), AS/3 (Nutricel), AS-5 (Optisol)

Answer 13

21 DAYS: CPD, ACD, CP2D
35 DAYS: CPD-A1
42 DAYS: CPD-AS-1 (Adsol), AS/3 (Nutricel), AS-5 (Optisol)

Question 14

WHOLE BLOOD
Characteristics:
* WBCs and Platelets - no longer viable after ____
* LABILE FACTORS (FACTORS ___ & ___) - significant ___ AFTER ___

Answer 14

• 24 HOURS
• V & VIII, decrease, 2 DAYS

Question 15

WHOLE BLOOD
Storage Temp:

Answer 15

1-6 degC (monitored temp)

Question 16

WHOLE BLOOD
Transport:

Answer 16

WET ICE w/ cloth & paper; do not put directly in ice

Question 17

WHOLE BLOOD
Immediate Effect of one unit:
Post-transfusion CBC:

Answer 17

ABOUT 30% INCREASE Hct PER 1 UNIT WB
AFTER 6 HOURS

Question 18

ASSOCIATED PROBLEMS AFTER WB TRANSFUSION

Answer 18

1. Circulatory overload in patients that require only oxygen-carrying capacities in the RBC
   PRBC - anemia or oxygen-carrying capacity
   WB - both oxygen-carrying capacity and volume expansion; only if there is a shortage of component
2. Not indicated to correct anemia for normal volemic patients - the additional volume of plasma is unnecessary and may cause volume-related complications

Question 19

CONTENTS:
PRBC -
LEUKODEPLETED RBCs -
WASHED RBCs -

Answer 19

CONTENTS:
PRBC - Hct should be 80% or less
LEUKODEPLETED RBCs - Residual WBCs
WASHED RBCs - Removal of plasma proteins, platelets, leukocytes and cellular debris

Question 20

STORAGE TEMP:
WHOLE BLOOD -
PRBC -
LEUKODEPLETED RBCs -
REJUVENATED RBCs -
WASHED RBCs -
FROZEN > THAWED > DEGLYCEROLIZED RBCs -

Answer 20

STORAGE TEMP:
WHOLE BLOOD - 1-6 degC
PRBC - 1-6 degC
LEUKODEPLETED RBCs - 1-6 degC
REJUVENATED RBCs - 1-6 degC
WASHED RBCs - 1-6 degC
FROZEN > THAWED > DEGLYCEROLIZED RBCs - 1-6 degC

Question 21

SHELF LIFE:
WHOLE BLOOD -
PRBC -
LEUKODEPLETED RBCs -
REJUVENATED RBCs -
WASHED RBCs -
FROZEN > THAWED > DEGLYCEROLIZED RBCs -

Answer 21

SHELF LIFE:
WHOLE BLOOD
21 days: CPD, ACD, CP2D
35 days: CPD-A1
42 days: CPD-AS-1 (Adsol), AS-3 (Nutricel), AS-5 (Optisol)

PRBC
Open System: Transfused within 24 hours; can be stored in refrigerator depending on the anticoagulant
Increased probability of product contamination; no integrally attached collection needles in the satellite bag
Closed System: Should NOT be exposed to air
If no PRBC → centrifuge WB to separate plasma and keep PRBC

LEUKODEPLETED RBCs
Closed System: depend on anticoagulant; same with other RBC components
Open System: 24 hours

REJUVENATED RBCs
Can be prepared 3 days after expiration of blood
Use of anticoagulant → New shelf life of rejuvenated RBC after addition of rejuvesol will be 24 HOURS

WASHED RBCs
Open System: valid for 24 hours

FROZEN > THAWED > DEGLYCEROLIZED RBCs
Frozen: 10 years
Deglycerolized: already gone thawing process; 24 hours