My research Flashcards
What are characteristics of epithelial cells
E-cadherin and cytokeratins
Characteristics of mesenchymal cells
fibronectin, N-cadherin, vimentin
EMT markers - increased proteins
Fibronectin Vimentin N-cadherin Snail Slug Twist Fox C2 Sox10 MMP2, MMP3, MMP9
EMT markers - decreased proteins
E-cadherin
cytokeratin
desmoplakin
occludin
EMT - functional markers
increased migration increased invasion increased scattering elongation of cell shape resistance to anoikis
define anoikis
programmed cell death
epigenetic regulation is?
constrain expression by adapting regions of the genome to maintain either gene silencing or gene activity. This is achieved through direct chemical modification of the DNA region itself and by modification of proteins that are closely associated with the locus.
posttranslational regulation
controlling the active protein levels - may be done by phosphorylation
post-transcriptional regulation
done by microRNAs after transcription to regulate gene expression
plasticity
the ability of a cell to take on characteristics of other cells in a tissue (EMT)
levels of zeb and emt
low = epithelila
med - EM
high = mesenchymal
levels of u200 and emt
low = mesenchymal
med - EMT
high = epithelial
snail level and emt
below 180K - epithelial
above 220K - mesenchymal
else any possible state
hif1 levels and metabolism since hif1 corresponds with hypoxia
high hif1 = warburg (anaerobic)
low hif1 = oxphos
med = W/O state
ampk levels and metabolism
low ampk = oxphos
high ampk = warburg
med = W/O
