Muscles and Neurons Flashcards
1
Q
Explain the structure of a nerve cell
A
a soma (cell body) and two types of processes: dendrites and usually a single axon.
2
Q
Inputs from other neurons (synaptic inputs) are received on the
A
dendritic tree and the soma.
3
Q
What is the difference between an action potential and a synaptic potential?
A
Synaptic potential goes towards the soma, while action potentials go away from the soma
4
Q
In communication, what are the two types of signals?
A
Electrical signals Chemical signals
5
Q
Where does electrical signalling happen?
A
dendrites, cell body, axon
6
Q
Where does chemical signalling happen?
A
synapses
7
Q
What is the membrane potential?
A
voltage across the cell membrane
8
Q
What is the membrane potential range in mV for a cell?
A
between –100 and +50 mV
9
Q
What is the resting membrane potential value?
A
Its value is usually between –50 and –70 mV (typically -65 mV).
10
Q
True or false: Almost all cells in the body have a negative resting membrane potential.
A
True
11
Q
Only x, y and some z can suddenly respond with a transient change of this potential (ie. with an action potential) in response to a stimulus – so they are excitable !
A
Only neurons, muscle fibres and some endocrine cells can suddenly respond with a transient change of this potential (ie. with an action potential) in response to a stimulus – so they are excitable !
12
Q
How are the intracellular potentials measured today ?
A
The microelectrode recording technique and The patch-clamp technique (additional feature of being able to measure current)
13
Q
What is the RMP definition?
A
Electrical potential difference (50 to 70 mV) across the cell membrane which results from separation of charge. There is more negative charges inside the cell in comparison to the extracellular fluid.
14
Q
The RMP is due to what three factors?
A
a) Unequal concentrations of Na+ and K+ inside and outside the cell b) Unequal permeability of the cell membrane to these ions [c) Electrogenic action of the Na-K pump – only a small contribution !]
15
Q
What is the Approximate concentrations of K+ and Na+ ions inside and outside neurons?
A
OUTSIDE [Na+] 150 mM [K+] 5 mM INSIDE [K+] 100 mM [Na+] 15 mM 
16
Q
Explain Ca2+ ion’s affect on the RMP:
A
Ca2+ ions do not affect the RMP as the membrane is not permeable to these ions at rest
17
Q
Explain Cl2- ion’s affect on the RMP:
A
Cl- ions also do not contribute to the RMP as their distribution is usually ’passive’ (i.e. in most neurones there are no active Cl- pumps).
18
Q
How do large ions affect RMP?
A
There are many negatively charged proteins inside the cell. However, since the cell membrane is not permeable to these large ions, they do not affect the RMP !
19
Q
State the Na/K pump ratio:
A
3 Na+ out 2 K+ in
20
Q
How is unequal permeability of the cell membrane to different ions (including Na+ and K+) explained ?
A
Two main types of ion channels (ie. channels which have selective permeability to ions) in neurons: a) Non-gated (‘leak’) channels - open at rest b) Gated channels (voltage-gated, ligand-gated*, or mechanically-gated) -usuallyclosed at rest
21
Q
In cell membrane of neurons, there are many leak __ channels, but very few leak___ channels.
A
In cell membrane of neurons, there are many leak K+ channels, but very few leak Na+ channels.
22
Q
At rest: PK+ / PNa+ ≈ where p means membrane permeability
A
40 / 1
23
Q
Explain The concept of the ‘Equilibrium potential’
A
An intracellular potential at which the net flow of ions is zero, in spite of a concentration gradient and permeability!
24
Q
The equilibrium potential can be calculated for each ion by the ‘Nernst equation’ What is this equation?
A
Eion = 61.5 mV x log ([ion]o / [ion]i)
25
The Nernst equation applies only to a situation when...
...a cell membrane is permeable only to one ion ! (ie. has leak channels only for one specific ion).
26
Glia cells have leak channels only for
K+ ions
27
The higher the permeability of the cell membrane to a particular ion,
the greater the ability of this ion to shift the RMP towards its equilibrium potential.
28
What is an example of the rule that 'The higher the permeability of the cell membrane to a particular ion, the greater the ability of this ion to shift the RMP towards its equilibrium potential'
At rest, in neurons the membrane permeability is much higher to K+ than to Na+; therefore the RMP is closer to the equilibrium potential for K+
29
Define the action potential:
The action potential is a very brief (lasting a few milliseconds in axons, longer in cell bodies) fluctuation in membrane potential caused by a transient opening of voltage-gated ion channels (mainly Na+ and K+) which spreads, like a wave, along parts of the neuron.
30
Explain hyperpolarization
If it becomes more negative (eg. changes from -70 to -75 mV) (the potential inside the cell moves closer to EK+ , and away from ENa+)
31
Explain depolarization
If it becomes less negative (eg. changes from -70 to -60 mV) (the potential inside the cell moves away from EK+ and closer to ENa+)
32
What are the three key stages of the action potential?
1. Fast depolarisation to about +30mV (‘reversal of polarisation’, or ‘overshoot’) after the membrane potential reaches threshold; 2. Repolarisation; 3. After -hyperpolarisation (AHP).
33
Information is coded in the _____ of action potentials.
Information is coded in the frequency of action potentials.
34
Briefly explain Depolarisation to threshold
Occurs by a stimulus (physical or chemical) Voltage-gated Na+ channels start to open near threshold (PK\>PNa)
35
Briefly explain Stage 1: Fast depolarisation
Voltage-gated Na+ channels open very fast PNa\>\>\>PK fast depolarisation to ≈ +30mV
36
Briefly explain Stage 2: Repolarisation
Na+ channels inactivate and voltage-gated K+ channels open PK\>\>\>PNa
37
Briefly explain Stage 3: After- hyperpolarisation
Voltage gated K+ channels remain open for a while and then close PK\>\>\>PNa then PK\>\>PNa
38
What is the absolute refractory period?
Fast depolarisation and Repolarisation
39
What is the relative refractory period?
after-hyperpolarisation
40
What counts as a stimulus?
physical (eg. electric current, light) or chemical (a drug or synaptic excitation)
41
Explain the ionic processes occurring during depolarisation
When MP reaches the threshold, there is a sudden activation (opening) of voltage-gated Na+ channels ( PNa+↑ ) - At this moment PK+ / PNa+ → 1 : 20 (before was 40 : 1); therefore MP shifts towards the ENa+ (ie. towards +60 mV) - Opening of voltage-gated Na+ channels is only short lasting, as these channels quickly inactivate !
42
Explain the ionic processes occurring during repolarisation
- depolarisation is followed by a transient opening of voltage-gated K+ channels, leading to repolarisation and AfterHyperPolarization (MembranePotential shifts towards EK+) Inactivation of voltage-gated Na+ channels and activation of voltage-gated K+ channels (since PK+/PNa+ becomes ≈ 100 : 1)
43
Explain Unmyelinated axons:
small diameter; transmission of APs slow, continuous
44
Explain Myelinated axons:
larger diameter, transmission of APs fast, saltatory
45
Explain the passive spread of current: (3 steps)
1. (Subthreshold) depolarisation at one region of the membrane (local) 2. Passive current flow (inside and outside the axon) (in a circular motion going from inside to outside, from outside to inside) 3. Depolarization of adjacent parts of membrane
46
How far does the passive spread of current spread from the initial point?
Approximately 1mm
47
Speed of AP transmission in unmyelinated axons:
1m/s
48
Why is speed of AP transmission in unmyelinated axons relatively slow?
‘Passive’ current flow between two adjacent points is fast, but AP must be regenerated at every point on the membrane. This takes time and therefore conduction velocity is slow.
49
Speed of AP transmission in myelinated axons:
20 to 100 m /sec
50
Myelin sheath formed in CNS:
by oligodendrocytes
51
Myelin sheath formed in PNS:
by Schwann cells
52
Myelination is discontinuous; interupted at
nodes of Ranvier
53
Myelination increases passive spread of current, how?
Due to the insulating properties of myelin, there is less current dissipation as it flows along the axon !
54
Passive conduction occurs in both directions -- True or false
True
55
How does Myelination increases action potential conduction velocity? (i.e. explain ‘saltatory conduction’)
Myelination increases speed of AP conduction by increasing efficiency of passive spread. Thus, AP need not be regenerated at every part of cell membrane. APs are generated only at nodes of Ranvier (current flows passively between nodes).
56
Can AP's travelling in opposite directions pass each other?
No they cannot as once an AP has passed a membrane it will be in the refractory period preventing further excitation. They will cancel each other – the process known as ‘collision’
57
AP transmitted from the cell body is in the what direction?
‘orthodromic’ direction
58
AP evoked by electrical stimulation of the axon, is transmitted in the what direction?
‘antidromic’ direction
59
How are APs generated in sensory neurons ?
First it evokes a graded depolarisation, known as ‘the receptor potential’. ● The receptor potential spreads passively to more distally located ‘trigger zone’ where APs are generated. ● APs then spread along the axon (myelinated or unmyelinated) towards the CNS.
60
How is Information about the strength of the stimulus coded in sensory neutrons?
the amplitude of the receptor potential and the frequency of APs.
61
How is a message transmitted from one excitable cell to another excitable cell?
a) Through chemical synapses - most often b) Through electrical synapses (eg. in the retina). Much less common !
62
Explain how synaptic transmission occurs from presynaptic membrane to post synaptic membrane:
presynaptic action potential reaches presynaptic knob there is an increased presynaptic Ca2+ permeability and thus a Ca2+ influx Then a release of a neurotransmitter by exocytosis occurs across the synaptic cleft to the post synaptic membrane then the neurotransmitters react with the post synaptic receptors which activates synaptic channels and causes a post synaptic action potential
63
What is an example of a neurotransmitter in a neuromuscular junction?
acetylcholine (ACh)
64
Endplate potential always triggers an action potential - t or f
true
65
What are the 2 main types of chemical synapses in the CNS?
Excitatory synapses and Inhibitory synapses
66
Explain Excitatory synapses
depolarisation of the postsynaptic membrane, called the Excitatory Postsynaptic Potential ( EPSP )
67
Explain Inhibitory synapses
hyperpolarisation of the postsynaptic membrane, called the Inhibitory Postsynaptic Potential (IPSP )
68
What are the neurotransmitters of excitatory synapses?
mainly glutamic acid (glutamate) or ACh
69
What are the Ionic mechanism of EPSPs?
transient opening of channels selective for Na+, K+ and sometimes Ca2+
70
What are the neurotransmitters of inhibitory synapses?
mainly GABA (gamma-aminobutyric acid) or glycine
71
What are the Ionic mechanism of IPSPs?
transient opening of K+ or Cl- channels
72
What are the two groups of Classification of neurotransmitters?
Small molecule neurotransmitters (‘Classical’ neurotransmitters) Neuropeptides (‘Neuromodulators’)
73
Give examples of Small molecule neurotransmitters
- Amino acids: glutamate, GABA, glycine - Acetylcholine (ACh) - Amines: serotonin (5-HT), noradrenaline, dopamine - ATP (Adenosine triphosphate)
74
Are small or large molecule neurotransmitters faster?
small, as it acts directly on postsynaptic receptors, while large neurotransmitters have an indirect ( ‘metabotropic’) action on postsynaptic receptors, or modulatory action on the effects of other neurotransmitters
75
What are examples of neuropeptides?
Neuropeptide Y (NPY), Substance P, Kisspeptin, Endorphins
76
What are the Factors determining synaptic action?
A) Type of neurotransmitter / neuromodulator B) Type of neurotransmitter receptor expressed in the postsynaptic membrane C) The amount of neurotransmitter receptor expressed in the postsynaptic membrane – ’Synaptic plasticity: LTP or LTD’
77
What are the 4 main subtypes of glutamate receptors?
AMPA, NMDA, Kainate and metabotropic glutamate receptor
78
What happens if too much glutamate is released?
Too much glutamate release leads to excessive depolarisation and overactivation of neurons. Long-term opening of NMDA receptors causes excessive Ca2+ entry that damages neurons- the process known as excitotoxicity.
79
What are three ways neurotransmitters are inactivated?
Re-uptake (for most of the aminoacids and amines) and re-cycling ! Diffusion away from the synapse Enzymatic degradation in the synaptic cleft (eg. Acetylcholine esterase degrades ACh ) Involvement of specific neurotransmitter transporters in the presynaptic membrane or adjacent glia cells; eg. ‘glutamate transporter’
80
The membranes of neurons at rest are very permeable to _____ but only slightly permeable to \_\_\_\_\_.
K+; Na+
81
The concentrations of which two ions are highest outside the cell.
Na+ and Cl–
82
What are the three main types of muscle?
1. Skeletal: attached to bones and is responsible for movement; 2. Cardiac: forms the bulk of the heart mass and its contraction ejects blood from the organ; 3. Smooth: mainly lines hollow organs and blood vessels, and regulates their dimensions.
83
Explain the characteristics of Skeletal Muscle
• Under voluntary control • Striated • Single long cylindrical cells • Multiple peripheral nuclei
84
Explain the characteristics of Cardiac Muscle
• Located only in the heart • Striated • Branched cells with 1-3 central nuclei • Connected via intercalated discs • Involuntary control
85
Explain the characteristics of Smooth Muscle
• Involuntary • Found in the wall of internal organs (gut, blood vessels etc) • Spindle shaped, uninucleated cells • Not striated
86
voluntary muscle being under control of the ______ and involuntary muscle under control of the \_\_\_\_\_\_\_\_.
voluntary muscle being under control of the somatic nervous system and involuntary muscle under control of the autonomic nervous system.
87
Skeletal muscle is Attached to bones via
tendons
88
How long can a muscle cell be?
35cm
89
How wide can a muscle cell be?
0.1mm
90
Draw the structure of a muscle fibre
91
Thick filaments:
run the entire length of an A band
92
Thin filaments:
run the length of the I band and partway into the A band
93
Zdisc
coin-shaped sheet of proteins that anchors the thin filaments and connects myofibrils to one another
94
H zone
lighter mid region
where filaments do not
overlap
95
M line
line of protein myomesin that holds adjacent thick filaments together
96
T tubules
deep invaginations continuous with the sarcolemma (cell membrane) and circle each sarcomere at each of the junctions of the A and I bands. Allows action potentials to be carried deep within the muscle cell
97
Sarcoplasmic reticulum
The calcium storage site. The terminal cisternae of the SR lie close to the T-tubules.
98
Explain the protein structure of myosin
Each myosin has 2 subunits each with a globular head and a tail, the two tails intertwine to form a helix
* The heads have a binding site for actin
* The head is an enzyme that hydrolyses ATP (an ATPase)
99
Explain the protein structure of thin filaments
Composed primarily of globular actin proteins
The filaments are composed of a double stranded helical actin chain (polymers).
Troponin and tropomyosin are regulatory proteins associated with actin in skeletal and cardiac muscle
100
Explain what happens when muscle contracts
The sarcomere shortens as the thin filaments are pulled over the thick filaments: -The Z-line is pulled toward the M-line
-The I band and H zone become narrower
101
Explain the 4 steps of the The cross bridge cycle
Cross-bridge formation
Powerstroke
Detachment
Energization of myosin head
102
Explain 1. Cross-bridge formation
-Myosin binds to the actin binding site to form a crossbridge
as ATP has been hydrolyzed to ADP and Pi
- Note: crossbridges can only occur in the presence of calcium when the myosin binding site on actin is exposed.
103
Myosic heads are what type of enzyme specifically?
The myosin heads are an ATPase, or an enzyme that hydrolyses ATP to ADP and Pi.
104
Explain the process of the 2) Power STroke
ADP is released
- The myosin head rotates to its low energy state (about 45° to the actin) pulling with it the thin filament
- The result is the shortening of the sarcomere
105
Explain 3) Detacthment
- A new ATP molecule binds to the myosin
- The actin-myosin bind is weakened and the myosin
detaches
106
Explain the 4. Energization of the myosin head
- Myosin head hydrolyzes the ATP to ADP + Pi
- The myosin head moves back to its “high energy” confirmation (about 90° to the actin)
107
How is calcium in skeletal muscle regulated?
In skeletal muscle opening of calcium channels in the SR allows the movement of calcium ions into the cytosol.
Active transport pumps (Ca2+ ATPase) are constantly moving Ca2+ from the cytoplasm back into the sarcoplasma reticulum
108
Describe the difference between Isometric vs isotonic
Isotonic (e.g. bicep curl)
Shortening
Tension constant
Velocity variable
Isometric (holding textbook)
No shortening
Length constant
Tension variable
109
When does the ball part of the channel block the channel?
Between depolarization and repolarization
110
WHat is the subthreshold voltage?
Before depolarization occurs, the voltage of the inside of the membrane must get more positive and if it reaches a certain threshold point an action potential will start, but if not (a subthreshold -55mV) , the action potential wont occur.
111
Where does electrical synapses occur? Give an example
Through gap junctions
112
What is the difference between spatial summation and temporal summation?
Spatial summation is when two neurons join at a point and their APs add together
While temporal summation is when one neuron's multiple APs send it in high frequency and they add together
113
What is the time it takes for calcium 2+ to diffuse across synaptic cleft?
0.5ms
114
WHat is an example of enzymes that degrade neurotransmitters?
Acetylcholerase
Mono amine oxidase
115
How do u remeber excitatory neurotrasmitters?
'Get excited about it ay mate'
ACh and glutamate
116
How do u remember inhibitory neurotransmitters?
'Dont get down about the glycine GABA"
GABA and Glycine
117
What is the triad?
The T tubule and two Sarcoplasmic reticulum around it
118
Ca2+ binds to ___ which holds ______ in place
Ca2+ binds to troponin which holds tropomyosin in place
119
What is the sarcolemma?
The layer around all the bundles of myofibrils
120
121
Explain the length tension relationship
During an isometric contraction –
At the level of the sarcomere the maximum force (tension developed) is dependent on the degree of actin and myosin overlap
122
Draw the lenght tension relationship graph
123
At lengths \<\_\_\_\_ filaments collide and interfere with each other reducing force developed
At lengths \<2.0 μm filaments collide and interfere with each other reducing force developed
124
At lengths\>\_\_\_\_\_active forces decline as the extent of overlap between filaments reduces, reducing the number of cross bridges
At lengths\>2.2μm active forces decline as the extent of overlap between filaments reduces, reducing the number of cross bridges
125
Maximal force between
Maximal force between 2.0 – 2.2 μm
126
WHat is passive force?
As muscle is stretched the connective tissue around the muscle cells resists the stretch = passive force.
127
How do you find out total tnsion?
Total tension is the sum of the active tension dependent on the sarcomere length and the passive tension
128
Under normal conditions, this is maximal at a sarcomere length of
Under normal conditions, this is maximal at a sarcomere length of 2.0 – 2.2 μm (2.0 – 2.2 x 10-6 m).
129
it is important to appreciate that during injuries, the possible range of sarcomere lengths may
increase.
130
The active force _____ as the extent of overlap between the filaments reduces, which ____ the number of possible cross bridge interactions along the sarcomere;
The active force declines as the extent of overlap between the filaments reduces, which reduces the number of possible cross bridge interactions along the sarcomere;
131
Definea motor unit
A motor unit consists of a motor neuron and all the muscle fibers it innervates.
132
Explain this step '1. ACh released into neuromuscular junction'
* An action potential travels down the motor neuron
* At the axon terminal Ca2+ channels open, and Ca2+ enters the axon terminal
* This triggers the vescicles containing ACh to fuse with the terminal membrane, releasing ACh into the neuromuscular junction (synaptic cleft)
133
Explainthis step: 2. Activation of ACh receptors
* The binding of ACh to the receptors on the muscle end plate causes opening of the ligand (ACh) gated ion channels.
* Opening of these channels allows movement of predominantly Na+ into the muscle cell making it less negative (end plate potential)
134
Why are the effects of Ach are short lasting?
the enzyme acetylcholinestarase rapidly breaks down Ach
135
Explain this step: 3. A Muscle Action Potential is triggered
* If sufficient ligand gated channels are opened the end plate potential reaches threshold
* Voltage gated Na+ channels open and an action potential is triggered
* The action potential is then propagated along the sarcolemma into the T tubule system
136
WHat is the difference between a skeletal muscle AP and a nerve cell AP?
Skeletal muscle has a resting membrane potential of -90mV (which is more negative than the RMP of nerve cells)
137
Explain this step: 4. Calcium is released from the SR
* The action potential is conducted down the t‐ tubules coming in close contact with the sarcoplasmic reticulum.
* This results in voltage gated Ca2+ channels in the sarcoplasmic reticulum opening.
* Ca2+ is then released into the cytosol
138
Explain this step: Ca2+ binds with troponin
• When Ca2+ concentrations reach a critical threshold the myosin binding sites on the actin filament are exposed allowing the cross‐bridge cycle to occur
139
Explain this step: Contraction ends when Ca2+ levels fall
* Calcium is actively pumped back into the sarcoplasmic reticulum via Ca2+‐ATPase pumps
* Troponin moves back covering the myosin binding site
140
What is the role of creatine phosphate?
For brief periods (\<15s) creatine phosphate can act as an ATP “store”
Creatine phosphate + ADP = creatine + ATP
Anaerobic
141
Explain the difference in type 1 and type 2 muscle fibres and their uses:
Type 1 is used for more aerobic activity, it has a small diameter and have high mitochondria and have good blood supply therefore look red. use by marathon runners.
Type 2 are used in powerlifters. They are larger in diameter and have less mitochondria as they mostly use anaerobic glycolysis
142
WHat is the regulation of force Dependent on?
* Rate of stimulation of individual motor units
* The number or motor units recruited
143
How long is the AP in a muscle fibre?
2ms
144
Explain the action of slow twitch vs fast twicth fibres:
Type 1 (“slow twitch”):
• Units with neurons innervating the slow efficient aerobic cells (maintaining posture, walking)
Type 2 (“fast twitch”):
• Units with the neurons innervating the large fibres that fatigue rapidly but develop large forces (jumping, weight lifting)
145
The efflux of calcium from the SR causes the myoplasmic calcium levels to ____ from about \_\_\_\_\_\_\_\_\_\_\_\_.
Calcium is then pumped back into the SR by a ___ \_\_\_\_\_, which uses ATP as an energy source (\_\_\_\_\_).
The efflux of calcium from the SR causes the myoplasmic calcium levels to increase from about 10–7M to 10–5M.
Calcium is then pumped back into the SR by a calcium pump, which uses ATP as an energy source (Ca-ATPase).
146
What is the eznyme called that transfers Pi from Creatine-Pi to ADP to give ATP?
creatine phosphokinase
147
Explain the role of adenylate kinase
ATP may also be formed from two molecules of ADP by adenylate kinase to give one molecule of ATP and one of AMP. (anaerobically)
148
Explain temporal sumation in terms of calcium levels in muscles
Since the duration of the action potential is much shorter than that of the calcium transient, additional action potentials can be initiated before the calcium levels return to resting levels (and the muscle has not relaxed).
This leads to the calcium levels remaining elevated and continuing force development. This effect is called temporal summation of the calcium transients and the force continues to rise until a higher steady level is achieved, called a tetanus.
Hence, the amount of force developed by a muscle depends both on the rate of stimulation of individual fibres as well as the number of fibres activated.
149
Draw a table comparing Type 1, Type 2A and type 2B muscle fibres on:
## Footnote
Max. ATPase Rate
SR Pumping Capacity
Diameter
Mitochondria/ Myoglobin/ Blood Supply
Glycolytic capacity
150
Thus, physical training can alter the composition of a muscle to match the demands placed upon it, by both changing
innervation patterns as well as the production of new muscle fibres (by division of existing fibres).
151
Sustained use leads to muscle ____ while a reduction in activity (due to a loss of innervation or sloth) leads to muscle \_\_\_\_\_.
Sustained use leads to muscle hypertrophy while a reduction in activity (due to a loss of innervation or sloth) leads to muscle atrophy.
152
153
Atrial cells are about \_\_\_\_μm long and \_\_\_μm in diameter with a central nucleus.
Atrial cells are about 100 μm long and 10 μm in diameter with a central nucleus.
154
Do ventircular msucles have t-tubules?
Yes
155
Do atrial cells have t tubules?
no
156
Ventricular cells are larger (typically __ μm long and __ μm in diameter)
Ventricular cells are larger (typically 100 μm long and 30 μm in diameter)
157
\_\_\_\_\_\_ prevent ventircular cells from separating during contraction
Desmosomes prevent cells from separating during contraction
158
Contain \_\_\_\_\_\_\_\_\_that allow the action potentials to be carried from one cell to the next
Contain gap junctions that allow the action potentials to be carried from one cell to the next
159
How long is the Ventricular myocyte action potential?
• Long lasting! \>100ms
160
Draw the ventricular myocyte action potential graph
161
Explain this graph
0 – rapid depolarisation due to fast voltage‐gated Na+ channel
2 – plateau phase due to slow voltage gated Ca2+ channel (L‐type Ca2+ channel)
3 – repolaristation due to closing of Ca2+ channels and opening of K+ (outward) channels
162
The heart has numerous ______ and large amounts of \_\_\_\_\_\_\_\_
The heart has numerous mitochondria and large amounts of myoglobin
163
Each cell has ______ nuclei and growth occurs mainly through _______ with relatively little cell division (after birth).
Each cell has one to three nuclei and growth occurs mainly through hypertrophy with relatively little cell division (after birth).
164
Explain cardiac muscle contraction
* Depolarization opens voltage‐gated fast Na+ channels in the sarcolemma
* Reversal of membrane potential from –90 mV to +30 mV
* Depolarization wave opens slow (L‐ type) Ca2+ channels in the sarcolemma (DHPR)
* Ca2+ surge prolongs the depolarization phase (plateau)
* Ca2+ influx balanced by a Na+/Ca2+ exchanger
* Ca2+ influx triggers opening of Ca2+‐ sensitive channels in the SR (RyRa), which liberates bursts of Ca2+ (i.e. calcium induced calcium release)
* E‐C coupling occurs as Ca2+ binds to troponin and sliding of the filaments begins (same as in skeletal muscle)
* Duration of the AP and the contractile phase is much greater in cardiac muscle than in skeletal muscle
* Calcium pumped out via SR Ca‐ATPase (SERCA) and extruded via Na+/Ca2+ exchanger
165
What does myogenic mean?
Cardiac muscle is myogenic – it initiates contractions without nervous input.
166
Action potentials are initiated in a group of specialised cells in the _____ \_\_\_\_\_\_\_ called the \_\_\_\_\_\_\_\_\_\_.
The action potential spreads throughout the atria and then via specialised conducting cells called _________ to the ventricles.
Action potentials are initiated in a group of specialised cells in the right atria called the sino-atrial node.
The action potential spreads throughout the atria and then via specialised conducting cells called Purkinje fibres to the ventricles.
167
Regation of Cardiac Output (CO) formula?
CO = stroke volume x Heart rate
168
Stroke volume (SV) reflects the tension developed by the cardiac muscle fibres in one contraction. Can be increased by:
* ‐ increased stretch of ventricles (length)
* ‐ increased rate of firing (heart rate/HR)
* ‐ certain hormones (e.g. Noradrenaline)
169
Explain this graph
1 Pacemaker potential: This slow depolarization is Mostly due to opening of Na+ channels.
2 Depolarization: At threshold, Ca2+ channels open. Explosive Ca2+ influx produces the rising phase of the action potential .
3 Repolarization is due to Ca2+ channels inactivating and K+ channels opening.
170
Explain Autonomic innervation of the heart.
171
How does epinephrin work?
Increases rate of spontaneous depolarization = increase heart rate
172
How does ACh work on the heart?
Decrease rate of spontaneous depolarization and decreases MDP = decrease heart rate
173
174
Why does Increasing heart rate increases contractile force (stroke volume)?
due to less time available for Ca2+ to be pumped out of cell
175
How does norandrenaline affect stroke volume?
Acts via β receptors on L‐ Type channels resulting in more calcium entering the cell.
‐Also acts on Ca2+ pump in SR so SR increases its Ca2+ stores
Net result = bigger/shorter contraction
176
Noradrenaline released by
sympathetic nerves
177
Smooth muscle cells are very _______ (typically ____ μm long and ______ in diameter) with a ___ central nucleus and tapered at the ends.
Smooth muscle cells are very long and thin (typically 100–400 μm long and 5–10 μm in diameter) with a single central nucleus and tapered at the ends.
178
They may be connected to each other by ‘\_\_\_\_\_\_\_\_’ or by ____ junctions and groups of cells may be ____ connected.
They may be connected to each other by ‘dense bodies’ or by gap junctions and groups of cells may be electrically connected.
179
What are the 2 Arrangements of smooth muscle in the walls of hollow organs
Longitudinal layer of smooth muscle
Circular layer of smooth muscle
180
Are there t tubules in smooth muscle?
No T‐tubules – caveolae instead (act to increase surface area)
181
What are the roles of dense bodies in smooth muscle?
Dense bodies act like z‐lines to “anchor” actin to sarcolemma
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No Troponin – the regulatory protein is instead
calmodulin
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How can smooth muscle be contracted?
neural, hormonal or spontaneous (myogenic)
1. Myogenic with slow waves of depolarisation (e.g. gut)
2. Neurally induced contraction (e.g ciliary and iris muscles)
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1. Calcium ions (Ca2+) enter the cytosol from the ECF via voltage‐ dependent or voltage‐ independent Ca2+ channels, or from the scant SR.
2. Ca2+ binds to and activates calmodulin.
3. Activated calmodulin activates the myosin light chain kinase enzymes.
4. MLCK activates the myosin ATPases.
5. Activated myosin forms cross bridges with actin of the thin filaments and shortening begins in the usual fashion.
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Explain Activation of Myosin by mysosin light chain kinase (MLCK)
Calmodulin binds with the myosin light chain kinase phosphoralating the light chains (e.g. LC20) located on the neck of the myosin, in turn activating the ATPase on the myosin
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How to turn on and off MLCK (Myosin light chain kinase) ?
– activated by calmodulin‐Ca2+
Phosphatase required to de‐phosphoralate the myosin light chain
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In smooth muscle Contraction ends when
a myosin light chain phosphatase dephosphoraltes the myosin light chain.
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Does Ca@= affect length of contraction?
Ca++ concentration does not determine the length of the contraction.
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Smooth muscle contraction is ____ regulated
Smooth muscle contraction is enzyme regulated
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When you stretch smooth muscle it will generally
1. Initially contract, effectively resisting the stretch
‐stretch activated calcium channels
2. Slowly relax, adapting to the change in length
‐ via calcium dependent K+ channels
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