Muscle - Lectures 10-11-12 Flashcards
what are the 3 types of muscle
- 2 similarities
- Skeletal muscle
- cardiac muscle
- smooth muscle
SIMILARITIES:
- excitability! membrane potential can be changed
- all use actin and myosin –> 2 major proteins responsible for contractability
describe each type of skeletal muscles:
- size?
- pattern/striation?
- how many nucleus?
- intercalated disk?
- T-tubules?
SKELETAL:
- large fibers
- striped or striated
- multiple nuclei –> advantage = can produce many proteins
- no intercalated disks
- T-tubules
CARDIAC:
- smaller than skeletal, branched
- striations
- 1 nucleus per cell
- cells are joined in series by intercalated disks (to squeeze at the same time)
- T-tubules!
SMOOTH:
- small
- no striations
- 1 nucleus per cell
- no intercalated disks
- no T-tubules
how can there be multiple nuclei in one muscle cell?
stem cells during embryonic development form myoblasts (1 nucleus per cell) –> merge together to form myocytes/muscle fibers = many nuclei per cell
what are antagonistic muscles?
- what are the 2 movements?
- move bones in opposite directions
- flexion moves bones closer together (arm curl)
- extension moves bones away from each other (push-up)
- muscle cells are called muscle _______- –> shape (2)
- what kind of cells differentiate into muscle for growth and repair?
- amount of muscle cells already decided at birth? what changes?
- muscle ____A____ –> bundle into _______ which bundle into _______
- connective tissues hold muscle to bone with _______
- muscle fibers –> long and cylindrical
- satellite cells/stem cells
- yes! when you exercise, you change diameter and length BUT stem cells can repair and replace damaged muscles
- muscle fibers bundle into fascicles which bundle into entire muscle
- tendon
what are the 3 layers of connective tissue that surround muscle vs fascicles vs myocytes?
- what does the connective tissue provide? (3)
- myocytes surrounded by endomysium
- fascicles surrounded by perimysium
- entire muscle surrounded by epimysium
- fluid, blood, nerves
Z-line vs M-line vs A band vs I-band vs H-zone
- dark or light?
- Z line/Z-disk: separates each sarcomere (btw 2 actins of different sarcomeres)
- M-line: middle of myosin
- A-band: entire length of myosin, has some overlap with actin –> dArk
- I-band: only covers actin (from 2 sarcomeres ish) –> light!
- H-zone: only myosin! bit lighter than A band
what is a sarcomere?
functional unit of muscle
muscle fiber anatomy:
- sarcolemma?
- sarcoplasm?
- sarcoplasmic reticulum? describe structure + function?
- sarcolemma: cell membrane
- sarcoplasm: cytoplasm
- sarcoplasmic reticulum: endoplasmic reticulum: longitudinal tubes with enlarged ends called terminal cisternae
- concentrates and sequesters Ca2+
What are t-tubules?
- what forms a triad?
- function?
- continuous with the sarcolemma, invaginations of the sarcolemma that allows action potentials to get closer to SR
- t-tubule + 2 flanking terminal cisternae = triad
- allow AP to penetrate nearer to the internal structures of the fiber
muscle fibers also contain
1. all sarcomeres linked together
2. energy source
3. powerhouse
- myofibrils
- glycogen granules
- mitochondria
- thin filament = which protein?
- thick filament = which protein? heavy vs light chains
- regulatory proteins (2)
- accessory proteins (2)
- crossbridges
- thin = actin –> each can interact with 3 myosin
- thick = myosin –> each can interact with 6 actin: heavy chains = motor domain = myosin ATPase VS light chains = regulatory fcts
- reg proteins: tropomyosin and troponin
- acces: titin and nebulin
myosin heads contain 3 parts
- actin binding site
- ATP or ADP/P+ binding site
- ATPase enzyme –> break down ATP into ADP + P
what are the 3 subunits of troponin?
- when troponin binds with ____ –> 2 things happen
- repeated as long as what?
- troponin I –> binds with actin
- troponin C –> binds with Ca2+
- troponin T –> binds with tropomyosin
- when troponin C binds with Ca2+ released by SR (from terminal cisternae), troponin pulls tropomyosin from myosin-binding sites of actin –> myosin binds tightly to and moves actin
- repeated as long as binding sites are uncovered and ATP is available
what is the important component of actin?
- actins are all linked together by _______
- myosin binding site! binds with myosin head and forms crossbridge
- myosin binding site usually covered by tropomyosin when not a lot of calcium
- linked together by tropomyosin ish (which covers myosin binding sites)
when myosin binds with actin, myosin heads move towards _______ –> sarcomere becomes longer/shorter
- A-band, H-zone, I band stay the same lengths?
- M-line –> sarcomere becomes shorter
- A band stays same
- I band and H-zone become shorter
- titan spans distance from _______ to the neighbouring ________
- nebulin attaches to a _______ but does not extend to the ________
- role of titn?
- from Z-disk to M-line (ie half a sarcomere)
- Z-disk but doesn’t extend to M-line
- titin provides elasticity and stabilizes myosin
- muscle tension = what?
- load = what?
- contraction = what?
- relaxation = what?
- muscle tension = force created by muscle
- load = weight or force opposing contraction
- contraction = creation of tension in muscle
- relaxation = release of tension
5 major steps leading up to skeletal muscle contraction
- events at the neuromuscular junction
- excitation-contraction (E-C) coupling
- Ca2+ signal
- contraction-relaxation cycle
- muscle twitch + sliding filament theory
do length or thick and thin filament change during contraction? –> what is this theory called?
- no!
- sliding filament theory of contraction –> overlapping actin and myosin fibrils
- fibrils are fixed length
- slide past each other in energy-dependant process
- what is a powerstroke?
- what happens at the end of a powerstroke (2)
- myosin crossbrige swivels and pulls actin toward M-line
1. myosin releases actin and resets and binds another actin
2. heads are not released in unison - then powerstroke is repeated many times
rigor state vs rigor mortis
- rigor state = occurs when no ATP or ADP are bound to myosin –> very brief
- rigot mortis: muscle freezes if no ATP is available to release myosin
Start: rigor state where myosin is tightly bound to ______ after a ______ _______ (needs what to detach?)
1. ATP binds and myosin ________ –> ATP increases/decreases myosin affinity to actin
2. _______ of ATP provides energy for myosin head to do what?
- how is energy provided?
3. ______ _______
- begins in response to ____ binding ______
- release of ____ allows head to swivel, pulling ____ toward ______
4. myosin releases ____ –> makes room for next ____
*CHECK SCHÉMA!
start: myosin bound to actin after a power stroke (needs ATP to detach!)
1. ATP binds to ATP binding site on myosin head and myosin detaches –> ATP decreases myosin affinity to actin
2. hydrolysis of ATP provides E for myosin head to rotate and weakly bind to actin
- E is provided by myosin ATPase that breaks down ATP into ADP and Pi
3. Power stroke
- to Ca2+ binding to troponin
- release of Pi allows head to pull actin towards M-line
4. myosin releases ADP –> makes room for next ATP
explain steps from neuron to contraction of muscle to relaxation (10 steps)
- somatic motor neuron releases Ach at neuromuscular junction
- ACh binds to Nm receptor –> opens Na+ channels –> depolarization -> EPP –> Action potential into T-tubules!
- AP in T-tubules alters conformation of DHP (L-type Ca2+ channel) receptor (voltage gated channel)
- DHP receptor opens RYR (Ca2+ channel on SR) in SR and Ca2+ from SR is released and enters cytoplasm
- Ca2+ binds to troponin C + expose myosin binding site
- myosin heads execute power stroke
- actin filament slides toward center of sarcomere
- sarcoplasmic Ca2+ ATPase pumps Ca2+ back into SR
- decrease in [Ca2+] in cytosol causes Ca2+ to unbind from troponin
- tropomyosin recovers binding site. when myosin heads release, elastic elements pull filaments back to their relaxed position
Timing of Excitation-Contraction coupling –> 3 steps
- motor neuron AP –> releases ACh –> binds Nm –> induces 2
- muscle fiber action potential –> Ca2+ released –> induces 3
- development of tension during 1 muscle twitch (latent period + contraction phase + relaxation phase)
skeletal muscle contraction requires a steady supply of ____
- what breakdown produces short burst of energy? using what enzyme?
- ________ are the most rapid and efficient store of energy?
- ATP!
- phosphocreatine breakdown –> creatine kinase
- carbohydrates (glucose)