MTAP BB Flashcards

1
Q

parental genes must separate randomly and equally into gametes during meiosis. no allele has an advantage over the other.

a. law of independent segregation
b. law of independent assortment
c. law of dominance

A

a. law of independent segregation

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2
Q

genes for different traits are inherited separately from each other. this allows for all possible combinations of genes to occur.

a. law of independent segregation
b. law of independent assortment
c. law of dominance

A

b. law of independent assortment

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3
Q

when two alleles of an inherited pair is heterozygous, the allele that is expressed is dominant, whereas the allele that is not expressed is recessive.

a. law of independent segregation
b. law of independent assortment
c. law of dominance

A

c. law of dominance

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4
Q

developed a mathematical formula that allowed the study of Mendelian inheritance in great detail.

a. G. Kohler & C. Milstein
b. G.H. Hardy & W. Weinberg
c. Rous & Turner

A

b. G.H. Hardy & W. Weinberg

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5
Q

one of the best tools for studying gene frequencies in human populations.

A

Hardy-Weinberg

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6
Q

genes are expressed equally.

A

codominant

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7
Q

50% of transfused RBCs should remain viable in the patient’s bloodstream for 24 hours.

true or false

A

false (75%)

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8
Q

requirement temperature for platelet concentrate and granulocyte concentrate.

A

20 - 24C

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9
Q

requirement temperature for FFP and cryoprecipitate.

A

-18C

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10
Q

requirement temperature if has red blood cell units

A

1 - 6C and 1 - 10C

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11
Q

decreased ATP level can cause damage to the cell membrane.

T or F

A

true

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12
Q

preservative of primary bags that has 35 days shelf life.

A

citrate-phosphate-adenine (CPDA1)

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13
Q

preservatives of primary bags that have/have 21 days shelf life. (3)

A
  1. acid-citrate-dextrose ACD
  2. citrate-phosphate-dextrose CPD
  3. citrate-phosphate-double dextrose CP2D
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14
Q

additive solutions prolong red cell survival up to ____ days.

A

42

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15
Q

additive solution:

isotonic.

A

saline

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16
Q

additive solution:

maintains ATP levels.

A

adenine

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17
Q

additive solution:

source of energy.

A

glucose

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18
Q

additive solution:

prevents storage-related hemolysis.

A

mannitol

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19
Q

can be added to expired RBC units and can extend up to 24 hours. (up to __ days after expiration)

A

rejuvenation solutions; 3 days

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20
Q

rejuvenation solution:

serves as buffer.

A

phosphate

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21
Q

rejuvenation solution:

Maintains 2,3-DPG levels.

A

Inosine and pyruvate

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22
Q

rejuvenation solution:

Maintains ATP levels.

A

adenine

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23
Q

it offers the longest shelf life (10 yrs.)

A

red cell freezing

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24
Q

red cell freezing requires what temperature.

A

-65C

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25
cryoprotective agent.
glycerol
26
primarily used for autologous units and storage of rare blood types.
red cell freezing
27
blood group antigens: inheritance is controlled by a gene or two closely linked genes. a. system b. collection c. high incidence series d. low incidence series
a. system
28
blood group antigens: antigens demonstrate serologic relationship but no independent inheritance. a. system b. collection c. high incidence series d. low incidence series
b. collection
29
blood group antigens: observed in > 90% of random population. a. system b. collection c. high incidence series d. low incidence series
c. high incidence series
30
blood group antigens: observed in < 1% of random population. a. system b. collection c. high incidence series d. low incidence series
d. low incidence series
31
an antibody is clinically significant when it reacts to what temperature.
37C
32
antibody occurs even without exposure to antigens. a. naturally occurring b. immune antibodies
a. naturally occurring
33
antibody occurs after exposure to RBC antigens. a. naturally occurring b. immune antibodies
b. immune antibodies
34
antibody type is IgM. a. naturally occurring b. immune antibodies
a. naturally occurring
35
antibody type is IgG. a. naturally occurring b. immune antibodies
b. immune antibodies
36
temperature reactivity is 4-22C. a. naturally occurring b. immune antibodies
a. naturally occurring
37
temperature reactivity is 37C. a. naturally occurring b. immune antibodies
b. immune antibodies
38
IgM is ___. a. warm reactive antibody b. cold reactive antibody
b. cold reactive antibody
39
IgG is ____. a. warm reactive antibody b. cold reactive antibody
a. warm reactive antibody
40
positive sign of antigen-antibody rxn: due to complement activation.
hemolysis
41
positive sign of antigen-antibody rxn: most common.
hemagglutination
42
grading of agglutination reaction: tube method one solid agglutinate; clear supernatant.
4+
43
grading of agglutination reaction: tube method presence of minor population of agglutinated cells superimposed on a negative background.
mixed field
44
grading of agglutination reaction: tube method several large agglutinates; clear supernatant.
3+
45
grading of agglutination reaction: tube method no agglutination.
0
46
grading of agglutination reaction: tube method medium-sized agglutinates; clear background.
2+
47
grading of agglutination reaction: tube method small agglutinates; turbid background.
1+
48
grading of agglutination reaction: gel method RBC agglutinates at the top of gel column.
4+
49
grading of agglutination reaction: gel method RBC agglutinates at the upper half of gel column.
3+
50
grading of agglutination reaction: gel method RBC agglutinates throughout the gel column.
2+
51
grading of agglutination reaction: gel method RBC agglutinates at the lower half of gel column.
1+
52
grading of agglutination reaction: gel method unagglutinated RBCs (pellet) at the bottom of gel.
0
53
grading of agglutination reaction: gel method presence of layer of agglutinated RBCs on top of the gel accompanied by a pellet of unagglutinated cells at the bottom of the tube.
mixed field
54
what is the optimal pH for agglutination reaction?
neutral pH
55
immunoglobulin that is most efficient in agglutination.
IgM
56
immunoglobulin that is poor in agglutination.
IgG
57
blood groups that show dosage.
MNS, Duffy, Lutheran, Kidd, Rh (except D)
58
media that helps detects IgG antibodies.
enhancement media/potentiators
59
potentiators: its advantage is shorter incubation time. a. LISS b. enzymes c. AHG
a. LISS
60
potentiators: Rh, Kidd, Lewis, I, P1 a. Enhances reaction b. Destroys reaction
a. Enhances reaction
61
potentiators: MNS, Duffy, Chido, Rodgers, Xg^a a. Enhances reaction b. Destroys reaction
b. Destroys reaction
62
immediate spin detects what immunoglobulin?
IgM
63
37C incubation detects what immunoglobulin?
IgG
64
Antiglobulin test detects what immunoglobulin?
IgG
65
Test mixture is centrifuged at room temperature. a. immediate spin b. 37C incubation c. Antiglobulin test
a. immediate spin
66
Sensitization of red cells occurs. a. immediate spin b. 37C incubation c. Antiglobulin test
b. 37C incubation
67
Contains antibodies that bind Fc portion of IgG, cross linking red cells a. immediate spin b. 37C incubation c. Antiglobulin test
c. Antiglobulin test
68
visible result of immediate spin.
agglutination
69
visible result of 37C incubation.
hemolysis
70
visible result of Antiglobulin test.
agglutination
71
"coombs test" a. immediate spin b. 37C incubation c. Antiglobulin test
c. Antiglobulin test
72
how to validate negative results in DAT and IAT?
check cells/ coombs' control
73
detects bound antibodies to RBC (in vivo sensitization of RBC). a. DAT b. IAT
a. DAT
74
detects serum antibodies. (in vitro sensitization of RBC ) a. DAT b. IAT
b. IAT
75
Specimen: Patient’s washed RBC a. DAT b. IAT
a. DAT
76
Specimen: Px’s serum + reagent red cells a. DAT b. IAT
b. IAT
77
does not require 37C incubation. a. DAT b. IAT
a. DAT
78
requires 37C incubation. a. DAT b. IAT
b. IAT
79
Ratio of serum to cells for antiglobulin test.
40:1
80
Inadequate washing of red cells causes ______ result.
false negative
81
Centrifugation required for antiglobulin test.
1000 RCF for 20 seconds
82
false results in AHG: clotted specimen. a. false positive b. false negative
a. false positive
83
false results in AHG: inadequate washing of cells. a. false positive b. false negative
b. false negative
84
false results in AHG: bacterial contamination of cells. a. false positive b. false negative
a. false positive
85
false results in AHG: inadequate incubation conditions. a. false positive b. false negative
b. false negative
86
false results in AHG: metal contamination of saline. a. false positive b. false negative
a. false positive
87
false results in AHG: old serum samples. a. false positive b. false negative
b. false negative
88
false results in AHG: dirty glasswares. a. false positive b. false negative
a. false positive
89
false results in AHG: serum/AHG is not added. a. false positive b. false negative
b. false negative
90
false results in AHG: over centrifugation. a. false positive b. false negative
a. false positive
91
false results in AHG: undercentrifugation. a. false positive b. false negative
b. false negative
92
false results in AHG: overreading. a. false positive b. false negative
a. false positive
93
false results in AHG: cell suspension too weak or too heavy a. false positive b. false negative
b. false negative
94
false results in AHG: contaminated AHG. a. false positive b. false negative
a. false positive
95
false results in AHG: non-reactive AHG. a. false positive b. false negative
b. false negative
96
ABO gene in chromosome ___.
9
97
H gene in chromosome __.
19
98
Secretor gene in chromosome __.
19
99
SeSe/Sese. a. Secretor b. Non secretor
a. Secretor
100
sese. a. Secretor b. Non secretor
b. Non secretor
101
N acetyl galactosamine. a. A antigen b. B antigen c. H antigen
a. A antigen
102
D galactose. a. A antigen b. B antigen c. H antigen
b. B antigen
103
L fucose. a. A antigen b. B antigen c. H antigen
c. H antigen
104
Formation: 37th day of fetal life. a. antigen b. antibody
a. antigen
105
Formation: start: 3-6 months. Peak: 5-10 yrs old. a. antigen b. antibody
b. antibody
106
Amorph or silent gene.
O gene
107
identify the blood type: Anti A = 4+ Anti B = 0 A1 cells = 0 B cells = 4+
type A
108
identify the blood type: Anti A = 0 Anti B = 4+ A1 cells = 4+ B cells = 0
type B
109
identify the blood type: Anti A = 4+ Anti B = 4+ A1 cells = 0 B cells = 0
type AB
110
identify the blood type: Anti A = 0 Anti B = 0 A1 cells = 4+ B cells = 4+
type O
111
identify the blood type: AA, AO
type a
112
identify the blood type: BB, BO
type B
113
identify the blood type: OO
type O
114
identify the blood type: AB
type AB
115
Determines the possible genotypes of an offspring after mating.
Abo matings:
116
determine the % of genotypes: ``` mother = AA father = BO ```
50% chance type AB | 50% chance type A
117
Is there a possibility of 4 different ABO blood types among the offspring?
yes
118
determine the % of genotypes: ``` Mother = AO Father = BO ```
25% chance for Type AB 25% chance for Type B 25% chance for Type A 25% chance for Type O
119
A subgroup: Antigens present: A and A1 a. A1 b. A
A1
120
A subgroup: Antigens present: A a. A1 b. A
b. A
121
common antibody of A1 and A.
anti-B
122
Reaction with Anti A1 lectin of A1.
4+
123
Reaction with Anti A1 lectin of A.
0
124
Source of Anti A1 lectin. a. Ulex europaeus b. Dolichos Biflorus
Dolichos Biflorus
125
Seed extracts that can agglutinate human RBCs
Lectin
126
rarest blood type.
Bombay phenotype
127
possible donor of Bombay phenotype.
only Bombay phenotype
128
hh sese a. Classical Bombay b. Para-Bombay
a. Classical Bombay
129
hh Se a. Classical Bombay b. Para-Bombay
b. Para-Bombay
130
Source of Anti H lectin. a. Ulex europaeus b. Dolichos Biflorus
a. Ulex europaeus
131
ABO typing discrepancies: missing/weakly reacting Antibody a. group 1 b. group 2 c. group 3 d. group 4
a. group 1
132
ABO typing discrepancies: missing/weakly reacting Antigen a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
133
ABO typing discrepancies: plasma protein abnormality a. group 1 b. group 2 c. group 3 d. group 4
c. group 3
134
ABO typing discrepancies: miscellaneous a. group 1 b. group 2 c. group 3 d. group 4
d. group 4
135
ABO typing discrepancies: Newborns, elderly a. group 1 b. group 2 c. group 3 d. group 4
a. group 1
136
ABO typing discrepancies: Cold reactive ab a. group 1 b. group 2 c. group 3 d. group 4
d. group 4
137
ABO typing discrepancies: Leukemia, Hodgkin's disease a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
138
ABO typing discrepancies: Multiple myeloma a. group 1 b. group 2 c. group 3 d. group 4
c. group 3
139
ABO typing discrepancies: Bruton’s agammaglobulinemia a. group 1 b. group 2 c. group 3 d. group 4
a. group 1
140
ABO typing discrepancies: A/B subgroups a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
141
ABO typing discrepancies: waldenstrom’s macroglobulinemia a. group 1 b. group 2 c. group 3 d. group 4
c. group 3
142
ABO typing discrepancies: Unexpected Ab a. group 1 b. group 2 c. group 3 d. group 4
d. group 4
143
ABO typing discrepancies: cis-Ab a. group 1 b. group 2 c. group 3 d. group 4
d. group 4
144
ABO typing discrepancies: Inc. fibrinogen levels a. group 1 b. group 2 c. group 3 d. group 4
c. group 3
145
ABO typing discrepancies: Acquired B phenomenon a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
146
ABO typing discrepancies: Hypogammaglobulinemia a. group 1 b. group 2 c. group 3 d. group 4
a. group 1
147
ABO typing discrepancies: Wharton’s jelly a. group 1 b. group 2 c. group 3 d. group 4
c. group 3
148
ABO typing discrepancies: Chimerism a. group 1 b. group 2 c. group 3 d. group 4
a. group 1
149
ABO typing discrepancies: Excessive amount of BgSS a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
150
ABO typing discrepancies: Excessive amount of BGSS a. group 1 b. group 2 c. group 3 d. group 4
b. group 2
151
occur when unexpected reactions are obtained in the forward and/or reverse grouping.
ABO discrepancies
152
``` Anti a = 4+ Anti b = 2+ A1 cells = 0 B cells = 4+ O cells = 0 AC = 0 ``` a. acquired B phenomenon b. Missing or weakly reacting antigens c. Missing or weakly reacting antibodies d. Cold reactive antibodies e. rouleaux formation
a. acquired B phenomenon
153
``` Anti a = 0 Anti b = w A1 cells = 4+ B cells = 0 O cells = 0 AC = 0 ``` a. acquired B phenomenon b. Missing or weakly reacting antigens c. Missing or weakly reacting antibodies d. Cold reactive antibodies e. rouleaux formation
b. Missing or weakly reacting antigens
154
``` Anti a = 4+ Anti b = 0 A1 cells = 0 B cells = 0 O cells = 0 AC = 0 ``` a. acquired B phenomenon b. Missing or weakly reacting antigens c. Missing or weakly reacting antibodies d. Cold reactive antibodies e. rouleaux formation
c. Missing or weakly reacting antibodies
155
``` Anti a = 2+ Anti b = 4+ A1 cells = 4+ B cells = 2+ O cells = 2+ AC = 2+ ``` a. acquired B phenomenon b. Missing or weakly reacting antigens c. Missing or weakly reacting antibodies d. Cold reactive antibodies e. rouleaux formation
d. Cold reactive antibodies
156
``` Anti a = R Anti b = R A1 cells = 4+ B cells = 4+ O cells = R AC = R ``` a. acquired B phenomenon b. Missing or weakly reacting antigens c. Missing or weakly reacting antibodies d. Cold reactive antibodies e. rouleaux formation
e. rouleaux formation
157
stack of coin appearance.
rouleaux formation
158
blood group: Also known as Rhesus factor since first experiment involved red cells of rhesus monkey.
Rh blood group
159
RHD gene a. Chromosome 1 b. Chromosome 6
a. Chromosome 1
160
RHCE gene a. Chromosome 1 b. Chromosome 6
a. Chromosome 1
161
RHAG gene a. Chromosome 1 b. Chromosome 6
b. Chromosome 6
162
5 Fisher race antigens.
D, C, c, E, e
163
Codes for production of D antigen. a. D gene b. d gene
a. D gene
164
Does not code for the production of D antigen. a. D gene b. d gene
b. d gene
165
convert wiener to fisher-race: Ro
Dce
166
convert wiener to fisher-race: R1
DCe
167
convert wiener to fisher-race: R2
DcE
168
convert wiener to fisher-race: Rz
DCE
169
convert wiener to fisher-race: r
dce
170
convert wiener to fisher-race: r’
dCe
171
convert wiener to fisher-race: r’’
dcE
172
convert wiener to fisher-race: r^y
dCE
173
convert fisher-race to blood factors: Dce
Rho hr’ hr’’
174
convert fisher-race to blood factors: DCe
Rho rh’ hr’’
175
convert fisher-race to blood factors: dce
hr’ hr’’
176
convert fisher-race to blood factors: dCe
h’ hr’’
177
convert fisher-race to blood factors: dcE
hr’ rh’’
178
convert fisher-race to blood factors: dCE
rh’ rh’’
179
Rosenfield: | c
Rh4
180
Rosenfield: | D
Rh1
181
Rosenfield: | e
Rh5
182
Rosenfield: | C
Rh2
183
Rosenfield: | E
Rh3
184
Convert fisher race to rosenfield: | Dce = Rh: 1, -2, -3, 4, 5
Rh: 1, -2, -3, 4, 5
185
Convert fisher race to Rosenfield: | Dce
Rh: 1, -2, -3, 4, 5
186
convert to ISBT: D
004 001
187
convert to ISBT: C
004 002
188
convert to ISBT: E
004 003
189
convert to ISBT: c
004 004
190
convert to ISBT: e
004 005
191
Adopted a 6-digit number for each authenticated blood group specificity. a. rosenfield b. fisher race c. ISBT
c. ISBT
192
symbol of weak D.
Du
193
Principle of weak D testing.
IAT
194
D gene is in opposite direction to C gene a. Genetic weak D b. C trans c. Partial D/D mosaic
b. C trans
195
Missing portion of D antigen molecule a. Genetic weak D b. C trans c. Partial D/D mosaic
c. Partial D/D mosaic
196
Only few antigens present on RBC a. Genetic weak D b. C trans c. Partial D/D mosaic
a. Genetic weak D
197
Rh antibodies may cause ___ and ___.
HTR and HDN
198
major Rh antigen and the most immunogenic.
D
199
Rh antigen: Most immunogenic to least immunogenic.
D>c>E>C>e
200
unusual phenotypes of Rh: Expressed on D+C+ red cells a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
f. G
201
unusual phenotypes of Rh: Absence of C/c and E/e a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
c. Exalted D (D--/D--)
202
unusual phenotypes of Rh: Partial suppression of Rh antigens a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
b. Rh mod
203
unusual phenotypes of Rh: C+e a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
e. rh i
204
unusual phenotypes of Rh: c+e a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
d. F
205
unusual phenotypes of Rh: Absence of all Rh antigens a. Rh null syndrome (---/---) b. Rh mod c. Exalted D (D--/D--) d. F e. rh i f. G
a. Rh null syndrome (---/---)
206
Le (a-b-) a. Non secretor b. Secretor c. Secretor or nonsecretor
c. Secretor or nonsecretor
207
Le (a+b-) a. Non secretor b. Secretor c. Secretor or nonsecretor
a. Non secretor
208
Le (a-b+) a. Non secretor b. Secretor c. Secretor or nonsecretor
b. Secretor
209
Le gene in chromosome ___.
chromosome 19
210
MNS blood group system: Mn antigens found on a. Glycophorin A b. glycophorin B
a. Glycophorin A
211
MNS blood group system: U antigen found on a. Glycophorin A b. glycophorin B
b. glycophorin B
212
MNS blood group system: Ss antigens found on a. Glycophorin A b. glycophorin B
a. Glycophorin A
213
MNS blood group system: Cold reactive/IgM (2 answers) a. Anti-M b. Anti-N c. Anti-S/ Anti-s d. Anti-U
a and b
214
MNS blood group system: Warm reactive/IgG (2 answers) a. Anti-M b. Anti-N c. Anti-S/ Anti-s d. Anti-U
c and d
215
MNS blood group system: which antibody is ph-dependent (prefers slightly acidic pH)? a. Anti-M b. Anti-N c. Anti-S/ Anti-s d. Anti-U
a. Anti-M
216
MNS blood group system: Common in S-s-U- individuals a. Anti-M b. Anti-N c. Anti-S/ Anti-s d. Anti-U
d. Anti-U
217
MNS blood group system: Common in S-s-U- individuals. a. Anti-M b. Anti-N c. Anti-S/ Anti-s d. Anti-U
d. Anti-U
218
P blood group system: what antigen deteriorates rapidly on storage of blood.
P1 antigen
219
Other sources of P1 antigen:
Hyatid cyst fluid | Pigeon eggs
220
Other sources of P1 antigen: | what is the source of one of the sources of P1 antigen?
Hyatid cyst fluid - E. granulosus Pigeon eggs common earth worm
221
P blood group system: Antibodies Most are cold reactive a. Anti-P1 b. Anti-P P1 Pk/Anti-Tj^a c. Auto Anti-P
a. Anti-P1
222
P blood group system: Antibodies Produced by “p” individuals a. Anti-P1 b. Anti-P P1 Pk/Anti-Tj^a c. Auto Anti-P
b. Anti-P P1 Pk/Anti-Tj^a
223
P blood group system: Antibodies Biphasic IgG (reacts at 30C and 37C) a. Anti-P1 b. Anti-P P1 Pk/Anti-Tj^a c. Auto Anti-P
c. Auto Anti-P
224
P blood group system: Antibodies Wide thermal range (IgM/IgG) a. Anti-P1 b. Anti-P P1 Pk/Anti-Tj^a c. Auto Anti-P
b. Anti-P P1 Pk/Anti-Tj^a
225
P blood group system: Antibodies Found in cases of Paroxysmal Cold Hemoglobinuria a. Anti-P1 b. Anti-P P1 Pk/Anti-Tj^a c. Auto Anti-P
c. Auto Anti-P
226
I blood group system: Rich in infant RBCs or cord cells a. I antigen b. i antigen
b. i antigen
227
I blood group system: Rich in adult RBCs a. I antigen b. i antigen
a. I antigen
228
I blood group system: Enhanced production in cases of M. pneumoniae infection. a. Auto anti-I b. anti-i
a. Auto anti-I
229
I blood group system: Associated with IM, myeloid leukemia, cirrhosis a. Auto anti-I b. anti-i
b. anti-i
230
I blood group system: Auto anti-I a. IgG/warm reactive b. IgM/cold reactive
b. IgM/cold reactive
231
I blood group system: anti-i a. IgG/warm reactive b. IgM/cold reactive
b. IgM/cold reactive
232
Top 3 immunogenic antigens:
1. A, B 2. Rh D 3. Kell antigen
233
Kell blood group system: Rarely encountered a. Anti-K b. Anti-k
b. Anti-k
234
Kell blood group system: ost common antibody after ABO and Rh a. Anti-K b. Anti-k
a. Anti-K
235
Duffy blood group system: Resistant to malarial invasion. a. Fy (a-b-) b. Fy (a+b-)
a. Fy (a-b-)
236
Duffy blood group system: Common in chinese. a. Fy (a-b-) b. Fy (a+b-)
b. Fy (a+b-)
237
Duffy blood group system: Common in African-American black population. a. Fy (a-b-) b. Fy (a+b-)
a. Fy (a-b-)