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Histopath Lec > Mounting and labeling > Flashcards

Mounting and labeling Flashcards

1
Q

last step in tissue processing that results in a
permanent histological preparation suitable for
microscopy, after adhesion of the sections on to
the slide and appropriate staining of the tissue.

A

Mounting

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2
Q

usually a syrupy fluid applied between the section and the
coverslip after staining, setting the section firmly,
preventing the movement of the coverslip

A

Mounting medium

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3
Q

important because it governs the contrast between the
cellular detail and the background, and also the
transparency of the observed sample against the bright
field of the microscope

A

Refractive index

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4
Q

Mounting media may be divided
into two main groups:

A
  • Aqueous Media
  • Resinous Media
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5
Q

used for mounting
sections from distilled water when the stains would be
decolorized or removed by alcohol and xylene

A

Aqueous mounting medium

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6
Q

Aqueous mounting medium is made up of

A

made up of gelatin, glycerin jelly or gum arabic, glycerol, sugar, and a preservative solution.

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7
Q

Purpose of glycerin jelly or gum arabic

A

to
solidify the medium

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8
Q

Purpose of glycerol

A

to prevent cracking and
drying of the preparation

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9
Q

Purpose of sugar

A

to increase the
refractive-index

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10
Q

has a low refractive index, is moderately transparent and
evaporates easily, hence is good only for temporary
mounting.

A

Water

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11
Q

improves the image
quality and also supports the specimen.

A

Refractive index of water

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12
Q

Why wet mounts cannot be stored over extended time period

A

water will evaporate

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13
Q

has a high index of refraction and provides greater
visibility if slightly diluted with water

A

Glycerin

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14
Q

Glycerin refractive index

A

1.46

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15
Q

miscible with water, is inexpensive, and is non-poisonous.

A

Glycerin

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16
Q

Disadvantage of glycerin

A
  • difficult to prepare slides that are truly permanent in
    nature.
  • slowly run off a slide that is tilted.
  • Do not stack slides for long as the pressure will squeeze
    glycerin from the mounts.
  • Glycerin will eventually evaporate and air will penetrate
    under the coverslip.
  • Glycerin can be attacked by microorganisms,
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17
Q

very suitable semi-permanent mounting medium

A

Glycerin

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18
Q

Commonly used to mount sections for immunofluorescence and glycerol may be added to other
agents to retard drying and cracking.

A

Phosphate buffered glycerol

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19
Q

Refractive index of Phosphate buffered glycerol

A

1.47

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20
Q

Formula of GLYCERIN JELLY (KAISER’S
1880)

A
  • Gelatin 10 gm.
  • Glycerol 70 ml.
  • Distilled water 60 ml.
  • Phenol crystals (preservative) 0.25 gm.
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21
Q

standard mounting medium used
when dehydration and clearing with xylene cannot be
made (as in fat stains)

A

Glycerin jelly

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22
Q

Glycerin jelly refractive index

A

1.47

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23
Q

FARRANT’S MEDIUM formula

A
  • Gum arabic 50 gm.
  • Distilled water 50 ml.
  • Glycerol 50 ml.
  • Sodium merthiolate 0.025 gm
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24
Q

FARRANT’S MEDIUM. Refractive index

A

1.43

25
Q

Advantage of farrant’s medium

A

does not solidify upon storage and therefore does not
need to be heated before use.

26
Q

Disadvantage of farrant’s medium

A

takes a longer time to harden and may therefore require
ringing

27
Q

Arsenic trioxide may be used as a substitute of sodium
merthiolate for preservation of the medium.

A

Farrant’s medium

28
Q

APATHY’S MEDIUM formula

A
  • Pure gum arabic (crystals not powder) 50 gm.
  • Pure cane sugar or sucrose 50 gm.
  • Distilled water 50 ml.
  • Thymol crystals 0.05 gm.
29
Q

Apathy’s medium refractive index

A

1.52

30
Q

used for methylene blue-stained nerve preparations and
as a general purpose aqueous mountant.

A

Apathy’s medium

31
Q

Advantage of apathy’s medium

A

one of the most useful aqueous mountants for fluorescent
microscopy, being virtually non- fluorescent.

32
Q

Disadvantage of apathy’s medium

A

sets quite hard, has a higher refractive index, and does not
require ringing.

33
Q

recommended for mounting frozen
sections from water.

A

BRUN’S FLUID

34
Q

BRUN’S FLUID formula

A
  • Glucose 24 gm.
  • Glycerine 6 ml.
  • Spirits of camphor 6 ml.
  • Distilled water 84 ml.
35
Q

used for preparations that have been
dehydrated and cleared in xylene or toluene, and are
recommended for majority of staining methods.

A

RESINOUS MOUNTING MEDIA

36
Q

RESINOUS MOUNTING MEDIA is divided into 2

A

natural and synthetic resins.

37
Q

most important synthetic resins are used for

A

embedding undecalcified bones,
and for electron
microscopy

38
Q

It is a transparent, almost colorless oleoresin that adheres
firmly to glass and sets to a hard consistency without
granulation.

A

Canada Balsam

39
Q

Canada Balsam refractive index

A

1.524

40
Q

recommended for whole mounts and
for thick sections because it does not shrink much.

A

Canada balsam

41
Q

may be added to Canada balsam to maintain its
neutral reaction

A

Calcium carbonate chips

42
Q

dissolved in xylene in an incubator at 37°C or paraffin
oven at 58 °C

A

Canada balsam

43
Q

Canadian tree:

A

Abus Balsamea

44
Q

recommended for small tissue sections but not for whole
mounts because of shrinkage produced on drying

A

DPX - (Dibutyl Phthalate and
Xylene)

45
Q

Disadvantage of DPX - (Dibutyl Phthalate and
Xylene)

A

It tends to set quickly and often retract from
the edge of the coverslip.

46
Q

colorless, neutral medium in which most standard stains
are well preserved.

A

DPX - (Dibutyl Phthalate and
Xylene)

47
Q

DPX - (Dibutyl Phthalate and
Xylene) refractive index

A

1.532

48
Q

synthetic resin mixture in xylene, available in a
pale yellow or colorless solution

A

XAM

49
Q

Xam refractive index

A

1.52

50
Q

Dries quickly without retraction, and preserves stains
well.

A

Xam

51
Q

CLARITE refractive index

A

1.544

52
Q

synthetic resin which is soluble in xylene

generally preferred over D.P.X

A

CLARITE

53
Q

Other recommended
synthetic mounting
media

A
  • Permount (made by Fisher Scientific)
  • H.S.R. (Harleco Synthetic Resin)
  • Clearmount (Gurr)
54
Q

process of sealing the margins of the cover-slip to prevent
the escape of fluid or semi-fluid mounts and evaporation
of mountant, to fix the coverslip in place, and to prevent
sticking of the slides upon storage.

A

Ringing

55
Q

The term “ringing” originated because

A

round coverslips
were initially used and the coating applied in the form of
a circle or “ring.”

56
Q

made up of two parts paraffin wax
mixed with 4-9 parts powdered colophonium resin,
heated and filtered.

A

Kronig cement

57
Q

cellulose adhesives

A

Durofix

58
Q

often used as a mountant in
immunofluorescence microscopy, has been
recommended as an alternative for glycerin jelly

A

Polyvinyl alcohol

Histopath Lec (7 decks)

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