More reptile objectives Flashcards
T/F: Venipuncture is a blind technique in reptiles
TRUE
Is aseptic preparation required for venipuncture?
YES
How much blood can be sampled at one time?
0.5ml/100g body weight
(Size and condition dictate; decrease volume in debilitated patients)
What are the anticoagulants of choice?
Lithium and sodium heparin
**EDTA causes RBC lysis in chelonians**
What possible contaminant should you be aware of during venipuncture?
Lymph contamination
What are the venipuncture sites for turtles, tortoises, and terrapins?
- Jugular
- Coccygeal
- Brachial
- Subcarapacial
- Interdigital vein (leatherbacks)
- Dorsal cervical sinus
What are the snake venipuncture sites?
- Caudal (ventral tail) vein
- Caudal to cloaca, 25-50% down tail, 40-60o angle on ventral midline
- Heart
- Located 22-33% from snout to vent (sedated)
How do you sample blood from the heart in a snake?
- Sedation, restraint in dorsal recumbency
- Palpate and immobilize
- Advance needle at 45o in craniodorsal direction into apex of beating ventricle
- Maintain digital pressure 30-60sec after this
Where are the venipuncture sites in lizards?
- Caudal tail vein
- Ventral midline 20-80% down tail, 45-90o
- Ventral abdominal vein
- Jugular vein
- Gives large sample
- Located behind tympanic membrane near level of the point of shoulder
Where is the crocodilian venipuncture site?
- Caudal ventral tail vein
- Small to medium
- Supravertebral
- Medium to large
What are the routine views for chelonian radiographs?
- Dorsoventral
- Lateral
- Craniocaudal
What are the various types of fluids used in reptilians?
- “Reptile ringers solution”
- One part LRS + two parts 2.5% dextrose and 0.45% NaCl
- Normasol-R
- LRS
What other colloid fluids can be used?
- Hetastarch
- Diluted 1:2 or 1:3 with 0.9% saline
- Given at a rate of 0.1ml/kg every 10-15min
- May be used in chelonians with severe shock from massive blood loss
- Oxyglobin
- Limited clinical use in sea turtles and desert tortoises without adverse affects
What fluid therapy routes are used for severely compromised chelonians?
- IV or IO routes of fluid administration
- Allow for rapid rehydration and emergency therapy
- IV or IO routes necessary for whole and artificial blood, colloid fluids, and fluids containing greater than 5% dextrose
Can the jugular vein be used for fluid administration?
Yes
In what species is the cervical sinus used for fluid therapy?
What are the advantages?
- Sea turtles
- Used for bolus IV fluid therapy
- Advantages
- Easy vessel accessibility
- Minimal stress to patient
- Repeated vascular access
What species/situations is the intracoelomic fluid therapy route used in?
- Maintenance therapy
- Injected through
- Chelonians–inguinal fossa
- Lizards–right side of lower abdomen
- Snakes–lower 1/3 of body
What are the advantages of the IC route for fluid therapy?
Technically easy
Allows administration of crystalloid fluids with up to 5% dextrose
What are the disadvantages of the IC route for fluid therapy?
- Fluids might not be absorbed rapidly
- Potential of compromising lung space or perforating the lungs, urinary bladder, or an ovarian follicle in mature females
- Hypoproteinemic patients may have fluid in the coelomic cavity (ascites/anasarca), which will further complicate absorption
What are the advantages of subcutaneous fluid administration?
- Technically easy
- Any available skin fold
- Chelonians: inguinal fossa, medially in the front limb fossa and the ventral neck fold
- Squamates: usually lateral body wall
- Also between and over scapula in lizards
What are the disadvantages of subcutaneous fluid administration?
- Poor absorption in severely debilitated chelonians
- Only 2.5% dextrose or less
When can oral fluids be administered? Which ones are administered first?
- Mild to moderate dehydration, maintenance
- Oral fluids consisting of electrolytes and dextrose administered first
How are oral fluids administered?
- Stomach tube using appropriately sized, well-lubricated red rubber or metal feeding tube
- Stomach volume in most chelonian patients is about 2% BW or 20ml/kg
- Anatomically, stomach is located in anterior 1/3 to mid-carapace in chelonians
When is soaking used as fluid administration? For how long?
- Rehydration
- Reduction of epibiota
- 24hrs
Can fluids be administered intra-cloacally?
Yes
What are the limits for volumes of fluid administration?
- Do NOT exceed 2-3% BW per day
- Maintenance = 15ml/kg/day
- Can go up to 30ml/kg/day
What are the indications for a blood transfusion?
- Life-threatening anemia, PCV <5%
- Acute hemorrhage