more about group 2 NSL

Question 1

Describe how lysine interacts with ATP

Answer 1

• Lys interacts with ATP gamma phosphate, the orientations are diff with ADP
• interacts via salt bridge
• loss of salt bridge causes large rearrangement in 160s loop

Question 2

Lys 161 as an atp sensor

Answer 2

• lys can sense atp in nucleotide sensing loop because it can form salt bridges. if atp is there, it will sense the gamma phosphate

Question 3

What does the rearrangement do

Answer 3

• rearrangement at interface between beta-hairpin motif and nucleotide sensing loop helps open entrance to the nucleotide-binding site (pocket)
• if have adp , entrance will open
• if have atp, forms a saltbridge and will not allow to have an open entrance
• opening and closing = conformational changes that allow to stabilze your complex

Question 4

motif

Answer 4

piece of a tertiary structure and interact to form a domain

Question 5

AMP-PNP

Answer 5

ATP mimic that jams reaction so that there cannot be a hydrolysis