chaperones intro

Question 1

chaperones are

Answer 1

several unrelated classes of proteins with different functions

Question 1

functions of chaperone proteins

Answer 1

• reverse denaturation
• prevent aggregation
• properly fold new proteins
• unfold proteins to transport through membranes then refold

Question 2

HSP 70 and HSP 40

Answer 2

• heat shock proteins
• released in response to higher temperatures
• HSP70 work with co-chaperone HSP40
• 70 and 40 kD

Question 3

chaperonins

Answer 3

• large multisubunit cages
• bind improperly folded globular proteins via exposed hydrophobic groups/surfaces

Question 4

2 chaperonin families

Answer 4

• Hsp60 (E. coli = GroEL) with 14 ~ 60 kD subunits in 2 rings of 7 subunits
• Hsp10 (in E. Coli = GroES) with single heptameric ring of 10 kD subunits