Monoclonal Antibodies

Question 1

N-linked glycans

Answer 1

Attached to nitrogen of asparagine, glutamine or arginine side-chains

Question 2

O-linked glycans

Answer 2

Attached to hydroxy oxygen of serine, threonine, tyrosine, hydroxylysine or hydroxyproline side chains

Question 3

How to quantitate presence of antigen

Answer 3

RIA (Radioimmuno assay), enzyme linked immuno-sorbent assay (ELISA)

Question 4

How to detect protein after fractionation by SDS Page

Answer 4

Western blotting

Question 5

Bispecific trifunctional mAb example

Answer 5

Catumaxomab

Question 6

Antibody fragments which lack the __ domain can be used for application where the antibody is only being used to block a signalling molecule/receptor

Answer 6

Fc domain

Question 7

Panning (also called biopanning)

Answer 7

Aka affinity enrichment. Technique for isolation of Ab fragments from a phage library based on their binding affinity to a given target

Question 8

The body of the phage particle is composed of major coat protein

Answer 8

gp8 (about 2800 copies)
Five copies of gp3 and gp6 at proximal end
Five copies of gp7 and gp9 (covers two ends of phage particle)