Molecular techniques Flashcards
1
Q
What is PCR and its function.
A
polymerase chain reaction
- amplify specific region of DNA from a trace sample and is done in vitro
for the amplification to occur, at least part of the sequence of the DNA sample must be known.
2
Q
what are the components in the PCR?
5
A
- DNA template
- Forward and reverse primers
- Thermostable Taq polymerase
- Excess free deoxyribonucleotides
- PCR reaction buffer
3
Q
describe the role of primers in PCR.
A
3
Q
Suggest why PCR may be needed before the ….
A
