Molecular Pathology

Question 1

Which of the following techniques is commonty used to detect specific DNA sequences in molecular
A. Western blotting
B. Polymerase chain roaction
C. Immunohistochemistry
D. Flow cytometry

Answer 1

B. Polymerase chain roaction

Question 2

Mutations in which of the following genes are commonly associated with hereditary breant and ovarian cancer
A. BRCA1 and BRCA2
В. ТР53
C. EGFR
D. KRAS

Answer 2

A. BRCA1 and BRCA2

Question 3

A translocation involving BCR and ABL genes is commonly associated with which type of leukeria?
A. Acute lymphoblastic leukemia (ALL)
B. Chronic lymphoblastic leukemia (CLL)
C. Acute myeloid leukemia (AML.)
D. Chronic myeloid leukemia (CML)

Answer 3

D. Chronic myeloid leukemia (CML)

Question 4

Which of the following is an example of a proto-oncogene that when mutated can contribute to the
development of cancer?
A. p53
B. RB
C. EGFR
D. BRCA1

Answer 4

C. EGFR

Question 5

The Philadelphia chromosome, resulting fror a translocation between chromosome 9 and 22 is characteristic
of which type of leukemia?
A. Acute lymphoblastic leukemia (ALL)
B. Chronic lymphocytic leukemia (CLL)
C. Acute myeloid leukemia (AML)
D. Chronic myeloid leukemia CML)

Answer 5

D. Chronic myeloid leukemia CML)

Question 6

Which of the following techniques is commonly used to assess DNA methylation patterns in genomic DNA?
A. Southern blotting
B. Methylation-specific PCR (MSP)
C. Fluorescent in situ hybridization (FISH)
D. Reverse transcription PCR (RT-PCR)

Answer 6

B. Methylation-specific PCR (MSP)

Question 7

Mutations in the BRAF gene are commonly found in which of the following types of cancer?
A. Breast cancer
B. Colorectal cancer
C. Lung cancer
D. Pancreatic cancer

Answer 7

B. Colorectal cancer

Question 8

In which of the following diseases is BRAF V600E mutation commonly found?
A. Colorectal cancer
B. Chronic myeloid leukemia
C. Lung cancer
D. Melanoma

Answer 8

D. Melanoma

Question 9

Which of the following is an example of a liquid biopsy used for molecular testing?
A. Bone marrow biopsy
B. Fine needle aspiration biopsy
C. Circulating tumor DNA (ctDNA) analysis
D. Histological section

Answer 9

C. Circulating tumor DNA (ctDNA) analysis

Question 10

10. Which of the following techniques can be used to analyze gene expression at the mRNA level?
    A. Southern blot
    B. Fluorescence in situ hybridization (FISH)
    C. Microarray analysis
    D. Flow cytometry

Answer 10

C. Microarray analysis