molecular biotech Flashcards
1
Q
what is agarose
A
polysaccharide derived from seaweed that forms a gel when dissolved in a heated aqueous solution
2
Q
what is gel electrophoresis
A
technique used to analyse and verify DNA fragments by separating mixtures according to their molecular size
3
Q
role of agarose
A
its meshwork of polymer fibres will impede the movement of DNA fragments, impeding longer fragments’ movement
4
Q
PCR components
A
oligonucleotide primers, template DNA, TAQ polymerase, buffer containing Mg2+, dNTPs
5
Q
purpose of buffer solution in gel electropherosis
A
contains ions which help conduct electricity in the electric field (?)
6
Q
A
