Molecular biology techniques

Question 1

SNOW DROP

Answer 1

SNOW (South - NOrth - West)
DROP (DNA - RNA - Protein)

Question 2

Southern blotting detects

Answer 2

Detects DNA

Question 3

Northern blotting

Answer 3

Detects RNA

Question 4

Western blotting

Answer 4

Detects proteins
Uses gel electrophoresis to separate native proteins by 3-D structure
Examples include the confirmatory HIV test

Question 5

direct visualisation of chromosomal anomalies what test is used ?

Answer 5

fluorescence in situ hybridisation (FISH)

• uses fluorescent DNA or RNA probe to bind to specific gene site of interest for direct visualisation of chromosomal anomalies