Molecular Biology Principles and Applications Flashcards
1978, proved that molecular scissors in bacteria, so-called restriction enzymes, recognize foreign DNA molecules and cut them at certain points.
Werner Arber
are the monomeric substrates for the polymerization reaction.
dNTPs (deoxynucleoside triphosphates)
separation of dsDNA to ssDNA, H bonds
a. denaturation
b. degradation
a. denaturation
b. degradation
between individual nucleotides, or phosphate backbone, breaks in the backbone of DNA molecule either DsDNA or ssDNA
a. denaturation
b. degradation
Causes of DNA degradation except
a. using very old DNA samples
b. leaving samples at room temp
c. protecting dna samples from heat
a. using very old DNA samples
b. leaving samples at room temp
c. protecting dna samples from heat
Short-term storage (weeks)
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
Long-term storage (years) under ethanol
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
Long-terms storage (decades)
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
a. 4 C in Tris-EDTA
b. -80 C in Tris-EDTA
c. -80 C as a precipitate
d. –164°C or dried
- characters that are relatively unchanged among taxa or certain group of organisms
Conserved
primary gene target for molecular detection of bacterial species as gene contains a mixture of conserved, variable and hypervariable regions.
16s rRNA gene
targets the highly conserved regions of the 16S and 23S rRNA genes.
Primers
general term for further differentiation below the species (type) or
subspecies level (subtype) based on a particular characteristic that is widely accepted per species:
Subtyping
“is the patient infected?”
test: qualitative virus infection testing
“what is the viral load?”
quantitative viral load testing
