Molecular Biology (DNA And Protein Synthesis) Flashcards
Purine
Double ringed
Guanine,adenine
Puga2
T-A
G-C
Pyrimidine
Single ringed
Thymine cytosine uracil
T-A
G-C
DNA replication 1
Helicase
Two parent strands of double helix are separated by enzyme called DNA HELICASE. Are where separated is called replication fork.
DNA replication 2
Binding proteins attach to unwound strands, preventing them from joining back together.
DNA replication 3
RNA (single stranded) are attached to the template strands and act as a starting point for replication.
DNA replication 4
DNA polymerase III synthesizes the new strands by adding free floating complementary nucleotides.
Central dogma
- dna replicates
- dna codes are transcribed in the production of mRNA through transcription. mRNA migrates from the nucleus to cytoplasm.
- mRNA carries coded information to ribosomes for use in protein synthesis through a process called translation.
DNA
Molecule type: double strand helix Sugar: deoxyribose Site: mostly in nucleus Metabolic rate: stable Base:thymine (g-c a-t)
RNA
Molecular type: single strand Sugar: ribose Site: throughout the shell Metabolic rate: unstable (active) Base:uracil (g-c a-u)
RNA: nuclear (nRNA)
Exact complementary strand of DNA including all the non coding pieces of information.
RNA: messenger (mRNA)
Functions in transferring important information coded in the DNA in the nucleus to the site of protein synthesis (cytoplasm)
Transcription steps
1:
2:
3:
Initiation: RNA polymerase binds to promoter( makes sure it binds at beginning of gene)
Elongation:an nRNA molecule forms in 5’ to 3’ direction nucleotides match the exposed DNA template
Termination:synthesis continues until it reaches the end of a gene.