Molecular Biology

Question 1

Nucleic Acid Testing (NAT)

Answer 1

Transcription-Mediated Amplification and Nucleic Acid Sequence-Based Amplification

Question 2

NAT allows specific sequences to be

Answer 2

multiplied rapidly and precisely

Question 3

Used to detect infectious pathogens for donor screenings for infectious agents:

Answer 3

HIV RNA
HCV RNA
HBV DNA
West Nile Virus RNA
Babesia RNA/DNA
Human T-cell lymphotropic virus, Hepatitis A, Parvovirus B19 (not widely available)

Question 4

recombinant proteins

Answer 4

proteins encoded by recombinant DNA that has been cloned in an expression vector that supports expression of the gene and translation of messenger RNA.

Question 5

Polymorphic DNA Sequences

Answer 5

RFLP - Restriction Fragment Length Polymorphisms
* VNTR - Variable Number Tandem Repeats ( >8 to more than 50 base pairs)
* STR - Short Tandem Repeats
(1- 8 base pairs)
* SNP- Single Nucleotide Polymorphisms