Molecular Bio Flashcards
which G-protein is responsible for activating AC and cAMP production?
Gs-alpha activates adenyl cyclase giving cAMP
which G-protein activates PLC instead of AC?
Gq-alpha proteins, they go on to PIP2 and IP3
RTK contains a SH2 in Grb2, what’s next?
SH3 from Grb2 binds to prolines in SOS, which binds and activates Ras. Activates the MAP kinase pathway, activates Erk
what molecules are associated with actin and function like gamma-turk allowing the nucleation of actin? For cell migration
Arp2 and Arp3 proteins
they require an activating factor
what is tau associated with?
microtubules, which are made of tubulin.
tau - binds microtubules, close packing of microtubules.
intermediate filaments
keratin
what’s the difference between cadherin and integrins?
cadherin is cell-cell contact
integrins is cell to basal lamina
basal lamina is at the base of the epithelium
what are connexins and where are they found?
connexins are found in gap junctions and connect cells electrically and metabolically
hydroxylases are required for the proper formation of what?
collagen - without it’s loose connective tissue. collagen is a triple helix made by hydroxylation events.
in what tissues are mitochondria not dynamic?
muscle tissues, specifically cardiac and around the flagellum of sperm
Helix-turn-helix
binds DNA as a dimer, the recognition motif is on the long helix, two alpha helices connect by a short chain of AA making a fixed angle.
zinc finger binding domains
contains Zn atom, binds major groove in DNA
leucine zipper motif
two alpha helices , dimers, has leucines every 7 AA
helix-loop-helix domain
two alpha chains connected by a loop of animo acids, either forms a homodimer or a heterodimer.
