Molecular And Genomic

Question 1

Recognition of the hereditary transmission of genetic information

Answer 1

Gregor mendel

Question 2

Application of genomics

Answer 2

Diagnosing and predicting
Detect errant genes
Exploration into fxn
Development of new gen therapeutics
Enhance immunity

Question 3

Preparation for DNA synthesis

Answer 3

G1

Question 4

Apoptosis can be activated by

Answer 4

Death receptor signals
Growth factor deprivation
DNA damage
Stress signals

Question 5

Monoclonal antibody that neutralizes the mitogenic activity of cell surface growth factor receptor HER-2

Answer 5

Trastuzumab

Herceptin

Question 6

Overexpressing tumors tend to grow faster and generally are more likely to recur

Answer 6

HER2

Question 7

Given for metastatic melanoma or kidney cancer

Mediate durable regression of metastatic cancer

Answer 7

Interleukin 2

Question 8

Primary function is to block different steps involved in cell growth and replication

Answer 8

STI57
Gleevec

Chemotherapy

Question 9

What are the limits of gene therapy

Answer 9

Improving selectivity of tumor targeting , delivery to the tumor and enhance of transduction rate of the cells

Question 10

Choice of appropriate genes for manipulation

Answer 10

Gene therapy

Question 11

Refers to the technique of transferring DNA fragments from an electrophoresis gel

Answer 11

Southern blot

Question 12

RNA and transferring in a solid support membrane

Answer 12

Northern drop

Question 13

Process of identifying a protein from a mixture of proteins

Answer 13

Western blot

Immunoblotting and precipitation

Question 14

Used to concentrate from a larger pool

Enrich protein of interest

Answer 14

Immunoprecipitation

Question 15

Gel used for blotting technique

Answer 15

S - agarose gel
N - formaldehyde agarose gel
W - PEG

Question 16

Target amplification of a specific dna sequence

Answer 16

PCR

Question 17

Remarkable ability of nuclei acids go form a duplex between two strands

Answer 17

DNA microarray

Question 18

Major applications of DNA microarray

Answer 18

Identification of sequence

Determination of expression level

Question 19

Based on shotgun sequencing

Illumina is an examle

Answer 19

Next generation sequencing

Question 20

One of the most powerful tools in biomedical lab

Answer 20

Cell culture

Question 21

Studying the regulation and function of gene

Presence or absence of serum

Answer 21

Cell transfection

Question 22

Studying gene function by creating mutant mice

Answer 22

Gene knockout

Question 23

What are the essential features of plasmid vectors

Answer 23

Origin of replication
Selectable markers
Unique restriction enzyme

Question 24

Type of host cell

Answer 24

E coli
Yeast
Other types of bacterium

Question 25

Vector plasmid taken up by treated bacterium

Answer 25

Transformation

Question 26

Brca 1

Ret proto oncogene

Chd 1 mutation

Answer 26

Mastectomy

Thyroidectomy

Gastrectomy