Modules 13 and 14

Question 1

Characteristic common to all non-fermentative Gram negative bacilli

Answer 1

Inability to ferment carbohydrates

Question 2

Why does the motility media method not work well for NFB?

Answer 2

Lack of oxygen prevents growth and spreading below the surface

Question 3

The most accurate method of determining flagellar arrangements on bacteria

Answer 3

Wet preparation

Question 4

Colour of positive OF tubes

Answer 4

Yellow

Question 5

Colour of negative OF tubes

Answer 5

Green

Question 6

3 most commonly isolated NFB organisms

Answer 6

Pseudomonas aeruginosa
Stenotrophomonas maltophilia
Acinetobacter baumannii

Question 7

Asaccharolytic

Answer 7

Does not utilize carbohydrates

Question 8

Three categories of bacteria based on their utilization of carbohydrates

Answer 8

Fermentative, oxidative, asaccharolytic

Question 9

Why would we use Mueller Hinton agar for NFB?

Answer 9

It is useful to demonstrate pigment production

Question 10

Typical flagellar arrangement for P. aeruginosa

Answer 10

Single flagellum at one end

Question 11

Pigment specific for P. aeruginosa

Answer 11

Pyocyanin

Question 12

P. aeruginosa reaction in glucose OF

Answer 12

Acid (yellow, positive) in open tube, no acid (green, negative) in closed tube

Question 13

Antimicrobials used to treat P. aeruginosa

Answer 13

Combination of a beta-lactam antibiotic and an aminoglycoside

Question 14

Typical flagellar arrangement of S. maltophilia

Answer 14

Three flagella at one end

Question 15

Species of Acinetobacter most often isolated from clinical material

Answer 15

baumannii

Question 16

Organism that smells like grape

Answer 16

Pseudomonas aeruginosa

Question 17

High cation concentration of divalent cations in susceptibility test media has what effect on results?

Answer 17

False resistant results