Modules 10, 11, 12 Flashcards
NLFs that produce H2S
Salmonella and Citrobacter
Which tests would differentiate a Salmonella from Citrobacter?
ONPG and Lys Salm = ONPG - , Lys + Citrobacter = ONPG + , Lys -
NLF genus that causes Bacillary Dysentery
Shigella
Two types of infections causes by Salmonella organisms
Enteric fever and Gastroenteritis
Why would Citrobacter be worked up as a possible Salmonella?
NLF at 24 hours, H2S positive
A lactose negative organism (genus) that produces H2S, is deaminase negative and lysine positive
Salmonella
Serotype of Salmonella that is ornithine negative and citrate negative
Salmonella typhi
Shigella dysenteriae group:
A
Shigella flexneri group:
B
Shigella boydii group:
C
Shigella sonnei group:
D
Group A Shigella
Shigella dysenteriae
Group B Shigella
Shigella flexneri
Group C Shigella
Shigella boydii
Group D Shigella
Shigella sonnei
Species of Shigella that is ONPG positive and Ornithine positive
Shigella sonnei
Common characteristics of the family Enterobacteriaceae
Gram negative rods, non-spore forming Facultative anaerobes with best growth in oxygen Oxidase negative Glucose fermeters Reduces nitrate to nitrite Catalase positive (most) Growth on MacConkey agar
The reaction in the butt of a TSI tube for Enterobacteriaceae
Acid (yellow); all Enterobacteriaceae ferment glucose
Top 3 Enterobacteriaceae commonly isolated from clinical material
Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis
An organism that forms dark pink dry colonies on MAC and is spot indole positive from colonies on BAP is most likely:
E. coli
Species of Enterobacter most often isolated from clinical material
Enterobacter cloacae
Two species of Klebsiella commonly isolated from clinical material
Klebsiella pneumoniae, Klebsiella oxytoca
Test to differentiate K. pneumoniae from K. oxytoca
Indole: K. pneumoniae indole neg, K. oxytoca indole pos
Genus that is almost always resistant to Ampicillin, Carbenicillin, and Ticarcillin
Klebsiella
Antimicrobial treatment for Klebsiella
Aminoglycosides (Gentamicin, Kanamycin, Tobramycin, Amikacin) and some Cephalosporins
Two species of Shigella most often isolated from clinical material
Shigella flexneri and Shigella sonnei
Most common species of Citrobacter
Citrobacter freundii
